Capsanthin supplementation modulates the immune response in broiler chickens under Escherichia coli lipopolysaccharide challenge

Abstract

Abstract. Due to the legislation of antibiotic usage, natural substances are required for application in the poultry industry. Because of their potential anti-inflammatory and immunomodulatory effects, carotenoids are great sources. Capsanthin, a major carotenoid giving the red color of pepper, is a promising feed additive, as it can reduce chronic inflammation. This study was conducted to determine the effects of capsanthin supplementation at 80 mg kg−1 in feed on the immune response of broiler chickens under Escherichia coli O55:B5 lipopolysaccharide (LPS) challenge. Ross 308 male broilers were divided into treatments: control (basal diet) and feed-supplemented groups. At 42 d of age, chickens were weighed and then challenged with 1 mg LPS per kilogram of body weight intraperitoneally. Four hours after injection, birds were euthanized, and then spleen and blood samples were collected. Capsanthin supplement at 80 mg kg−1 did not change the growth parameters and the relative spleen weight. LPS immunization resulted in higher splenic interleukin-1β (IL-1β), interleukin-6 (IL-6), and interferon-γ (IFN-γ) mRNA expressions. Capsanthin addition reached lower gene expression levels of IL-6 and IFN-γ compared to the LPS-injected birds. At plasma level, dietary capsanthin resulted in lower IL-1β and IL-6 levels. These results may indicate the potential anti-inflammatory effect of capsanthin supplementation in broiler chickens.

Embryonic methionine triggers post-natal developmental programming in Japanese quail

“…At this time, plasma IGF-1 levels and RPS6K1 gene expression increased in L-methionine-treated individuals compared to controls, while mTOR and IGF1 gene expression were no longer different between the treatment groups. These unmatched protein and gene expression suggest that proteins may be post-transcriptionally regulated, such as stability of mRNA or elevated half-life of the protein resulting from the post-translational modifications often altering the protein levels (Csernus et al 2023 ; Ideker et al 2001 ). After hatching, an increased hepatic IGF1 mRNA expression may lead to increased production of IGF-1 protein.…”

Growth performance, carcass composition, physico-chemical traits and amino acid profile of meat depending on wormwood (Artemisia absinthium L.) dietary supplementation in broilers

“…The ban on antibiotic growth-promoting compounds in poultry diets in Europe since 2006 has led to the search for natural alternatives, improving performance, protecting animal health and providing profitable production. Regarding the safety of the origin of meat, the supplementation of animal feed with natural phytogenic additives appears to be very promising (Puvača et al, 2014;Csernus et al, 2023). Probiotics, prebiotics, organic acids and herbs, as well as herbal essential oils or aromatic plants and their extracts, have been added in animal feed as alternatives to synthetic growth promoters (Kostadinović and Lević, 2012;Amin et al, 2022;Hassan et al, 2022;Gumus and Gelen, 2023).…”