Capturing rare cells from blood using a packed bed of custom-synthesized chitosan microparticles

Arya, Chandamany; Kralj, Jason G.; Jiang, Kunqiang; Munson, Matthew; Forbes, Thomas P.; DeVoe, Don L.; Raghavan, Srinivasa R.; Forry, Samuel P.

doi:10.1039/c3tb20818d

Cited by 15 publications

(21 citation statements)

References 27 publications

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“…5a where the inner layer is nonionic while the outer layer is anionic. To make this capsule, we first created a pH-insensitive core of chitosan (an amine-rich polysaccharide) cross-linked with glutaraldehyde (GA) 36 , 49 . The core was made as before by adding the chitosan solution drop-wise into a solution of the GA; note that GA forms covalent bonds between the amines on chitosan 32 .…”

Section: Resultsmentioning

confidence: 99%

Onion-like multilayered polymer capsules synthesized by a bioinspired inside-out technique

Zarket

Raghavan

2017

Nat Commun

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Diverse structures in nature, such as the spinal disc and the onion have many concentric layers, and are created starting from the core and proceeding outwards. Here, we demonstrate an inside-out technique for creating multilayered polymer capsules. First, an initiator-loaded gel core is placed in a solution of monomer 1. The initiator diffuses outward and induces polymerization, leading to a shell of polymer 1. Thereafter, the core-shell structure is loaded with fresh initiator and placed in monomer 2, which causes a concentric shell of polymer 2 to form around the first shell. This process can be repeated to form multiple layers, each of a distinct polymer, and of controlled layer thickness. We show that these multilayered capsules can exhibit remarkable mechanical resilience as well as stimuli-responsive properties. The release of solutes from these capsules can be tailored to follow specific profiles depending on the chemistry and order of adjacent layers.

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Section: Resultsmentioning

confidence: 99%

Onion-like multilayered polymer capsules synthesized by a bioinspired inside-out technique

Zarket

Raghavan

2017

Nat Commun

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“…Besides, the performances of bead-packed based filtration for CTCs isolation process too are discussed in the literature. Such a method is typically incorporated with the capillary-driven analysis systems, whereby a batch of uniform (diameter of 45 μ m) [ 30 ] and nonuniform beads (diameter ranged from 100 μ m to 15 μ m) [ 16 ] are packed into a chamber and act as the filter element ( Figure 2(g) ). Subsequently, when the blood is channeled into the filtration chamber inlet, blood cells such as RBC and WBC are allowed to flow through the packed-bead whereas CTCs will be immobilized within the packed bed.…”

Section: Benchtop Technologies For Ctcs Isolationmentioning

confidence: 99%

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Low

Abas

2015

BioMed Research International

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Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate. Despite its clinical relevance, the isolation and characterization of CTCs can be quite challenging due to their large morphological variability and the rare presence of CTCs within the blood. Numerous methods have been employed and discussed in the literature for CTCs separation. In this paper, we will focus on label free CTCs isolation methods, in which the biophysical and biomechanical properties of cells (e.g., size, deformability, and electricity) are exploited for CTCs detection. To assess the present state of various isolation methods, key performance metrics such as capture efficiency, cell viability, and throughput will be reported. Finally, we discuss the challenges and future perspectives of CTC isolation technologies.

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“…These specimens further represent a good model to mimic circulating tumor cells in the blood stream of breast cancer patients. 27 These specific cell line cells have been selected mainly due to their wide availability and not because of any obvious differences in the morphology.…”

Section: Sample Preparationmentioning

confidence: 99%

Cancer cell classification with coherent diffraction imaging using an extreme ultraviolet radiation source

et al. 2014

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Abstract. In cancer treatment, it is highly desirable to classify single cancer cells in real time. The standard method is polymerase chain reaction requiring a substantial amount of resources and time. Here, we present an innovative approach for rapidly classifying different cell types: we measure the diffraction pattern of a single cell illuminated with coherent extreme ultraviolet (XUV) laser-generated radiation. These patterns allow distinguishing different breast cancer cell types in a subsequent step. Moreover, the morphology of the object can be retrieved from the diffraction pattern with submicron resolution. In a proof-of-principle experiment, we prepared single MCF7 and SKBR3 breast cancer cells on gold-coated silica slides. The output of a laser-driven XUV light source is focused onto a single unstained and unlabeled cancer cell. With the resulting diffraction pattern, we could clearly identify the different cell types. With an improved setup, it will not only be feasible to classify circulating tumor cells with a high throughput, but also to identify smaller objects such as bacteria or even viruses.

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Capturing rare cells from blood using a packed bed of custom-synthesized chitosan microparticles

Cited by 15 publications

References 27 publications

Onion-like multilayered polymer capsules synthesized by a bioinspired inside-out technique

Onion-like multilayered polymer capsules synthesized by a bioinspired inside-out technique

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Cancer cell classification with coherent diffraction imaging using an extreme ultraviolet radiation source

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