Carbapenem-Resistant<i>Enterobacter</i>spp. in Retail Seafood Imported from Southeast Asia to Canada

Janecko, Nicol; Martz, Sarah-Lynn; Avery, Brent P.; Daignault, Danielle; Desruisseau, Andrea; Boyd, David A.; Irwin, Rebecca; Mulvey, Michael R.; Reid‐Smith, Richard J.

doi:10.3201/eid2209.160305

Cited by 56 publications

(44 citation statements)

References 8 publications

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“…Consequently, E. cloacae has recently been used as a hygiene indicator in foodstuff processing, and is also one of the most challenging bacterial contaminants of raw and processed meat products, and vegetables [3]. Alarmingly, carbapenem-resistant E. cloacae (CREC) that can produce resistance to the last-resort carbapenem antibiotics and compromise these drugs for the treatment of life-threatening infections [4], has been recently detected in seafood originating from Southeast Asia, such as shrimp and clams [5]. This finding indicates that the risk for exposure to CREC extends beyond persons with particular travel histories, previous antimicrobial drug use, or hospitalization, and into the general public.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

Qian

Liu

et al. 2019

Antibiotics

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Vanillic acid (VA) is a flavoring agent found in edible plants and fruits. Few recent studies exhibited robust antibacterial activity of VA against several pathogen microorganisms. However, little was reported about the effect of VA on carbapenem-resistant Enterobacter cloacae (CREC). The purpose of the current study was to assess in vitro antimicrobial and antibiofilm activities of VA against CREC. Here, minimum inhibitory concentrations (MIC) of VA against CREC was determined via gradient diffusion method. Furthermore, the antibacterial mode of VA against CREC was elucidated by measuring changes in intracellular adenosine triphosphate (ATP) concentration, intracellular pH (pHin), cell membrane potential and membrane integrity. In addition, antibiofilm formation of VA was measured by crystal violet assay and visualized with field emission scanning electron microscopy (FESEM) and confocal laser scanning microscopy (CLSM). The results showed that MIC of VA against E. cloacae was 600 μg/mL. VA was capable of inhibiting the growth of CREC and destroying the cell membrane integrity of CREC, as confirmed by the decrease of intracellular ATP concentration, pHin and membrane potential as well as distinctive variation in cellular morphology. Moreover, crystal violet staining, FESEM and CLSM results indicated that VA displayed robust inhibitory effects on biofilm formation of CREC and inactivated biofilm-related CREC cells. These findings revealed that VA exhibits potent antibacterial activity against CREC, and thus has potential to be exploited as a natural preservative to control the CREC associated infections.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

Qian

Liu

et al. 2019

Antibiotics

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“…The following carbapenemase genes were [19,35,36,48e50], and imipenem-carbapenemase (IMP-1, IMP-2, IMP-27) ( (Table 1). Overall, carbapenemase-producing CRE were isolated from poultry (meat) [25,36], chicken, poultry, or fowl (meat) [19,22,24,26e28,34,40,50], pig (meat) [29,39e42,46,48,60], cows or raw milk [18,30,31,44,49], cattle [35], and various types of seafood [62,64,65].…”

Section: Occurrence Of Cre In Livestock and (Sea)foodmentioning

confidence: 99%

“…If no selective screening approach to detect CR isolates is used as an initial diagnostic step, this limits interpretability of the data with respect to the prevalence of CRE in the tested samples because of a lack of sensitivity. Thus, for drawing conclusions about the prevalence of CRE, we extracted data from selective screening studies (Table S5), including 16 that screened samples from livestock [18,19,23,25,28,30,37,46,49e51,55,56,58e60], two from seafood [62,63], five from companion animals [25,67,68,70,73], and six from wildlife [25,77e79,81,82]. Although most studies included only few samples and cannot be considered as representative, the prevalence of CRE among livestock seemed to be low (<1%) in European countries (The Netherlands, Switzerland, UK), while some recent studies indicated a higher prevalence in Asia (China: 15% in milk samples, India: 1e3% in piglets, Lebanon: 2.5% in fowl) and Algeria (6% in milk samples, 26% in chickens).…”

Section: Prevalence Of Crementioning

confidence: 99%

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Köck

Daniels-Haardt²,

Becker

et al. 2018

Clinical Microbiology and Infection

261

167

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“…found in environmental samples such as sewage, tap water, and foodstuffs, posing a severe threat to public health (2)(3)(4).…”

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confidence: 99%

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Sugawara

Akeda

Hagiya

et al. 2019

Antimicrob Agents Chemother

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The spread of carbapenemase-producing Enterobacteriaceae (CPE), contributing to widespread carbapenem resistance, has become a global concern. However, the specific dissemination patterns of carbapenemase genes have not been intensively investigated in developing countries, including Myanmar, where NDM-type carbapenemases are spreading in clinical settings. In the present study, we phenotypically and genetically characterized 91 CPE isolates obtained from clinical (n = 77) and environmental (n = 14) samples in Yangon, Myanmar. We determined the dissemination of plasmids harboring genes encoding NDM-1 and its variants using whole-genome sequencing and plasmid analysis. IncFII plasmids harboring blaNDM-5 and IncX3 plasmids harboring blaNDM-4 or blaNDM-7 were the most prevalent plasmid types identified among the isolates. The IncFII plasmids were predominantly carried by clinical isolates of Escherichia coli, and their clonal expansion was observed within the same ward of a hospital. In contrast, the IncX3 plasmids were found in phylogenetically divergent isolates from clinical and environmental samples classified into nine species, suggesting widespread dissemination of plasmids via horizontal transfer. Half of the environmental isolates were found to possess IncX3 plasmids, and this type of plasmid was confirmed to transfer more effectively to recipient organisms at a relatively low temperature (25°C) compared to the IncFII plasmid. Moreover, various other plasmid types were identified harboring blaNDM-1, including IncFIB, IncFII, IncL/M, and IncA/C2, among clinical isolates of Klebsiella pneumoniae or Enterobacter cloacae complex. Overall, our results highlight three distinct patterns of the dissemination of blaNDM-harboring plasmids among CPE isolates in Myanmar, contributing to a better understanding of their molecular epidemiology and dissemination in a setting of endemicity.

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Carbapenem-ResistantEnterobacterspp. in Retail Seafood Imported from Southeast Asia to Canada

Cited by 56 publications

References 8 publications

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

RETRACTED: In Vitro Antibacterial Activity and Mechanism of Vanillic Acid against Carbapenem-Resistant Enterobacter cloacae

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

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