Carbapenem Susceptibility and Multidrug-Resistance in Pseudomonas aeruginosa Isolates in Egypt

Abstract

BackgroundResistant Pseudomonas aeruginosa is a serious concern for antimicrobial therapy, as the common isolates exhibit variable grades of resistance, involving beta-lactamase enzymes, beside native defense mechanisms.ObjectivesThe present study was designed to determine the occurrence of Metallo-β- Lactamases (MBL) and Amp C harboring P. aeruginosa isolates from Suez Canal university hospital in Ismailia, Egypt.MethodsA total of 147 P. aeruginosa isolates, recovered from 311 patients during a 10-month period, were collected between May 2013 and February 2014; the isolates were collected from urine, wound and sputum. Minimum inhibitory concentration (MIC) determined by agar dilution methods was ≥2 μg/mL for meropenem and imipenem. Identification of P. aeruginosa was confirmed using API 20NE. Metallo-β- Lactamases and Amp C were detected based on different phenotypic methods.ResultsOverall, 26.5% of P. aeruginosa isolates (39/147) were carbapenem resistant isolates. Furthermore, 64.1% (25/39) were MBL producers, these isolates were screened by the combined disc and disc diffusion methods to determine the ability of MBL production. Both MBL and Amp C harbored P. aeruginosa isolates were 28% (7/25). Sixty-four percent of P. aeruginosa isolates were multidrug resistant (MDR) (16/25). The sensitivity toward polymyxin, imipenem, norfloxacin, piperacillin-tazobactam and gentamicin was 99%, 91%, 88%, 82% and 78%, respectively. The resistance rate towards cefotaxime, ceftazidime, cefepime, aztreonam and meropenem was 98.6%, 86%, 71.4%, 34% and 30%, respectively.ConclusionsMultidrug resistance was significantly associated with MBL production in P. aeruginosa. Early detection of MBL-producing P. aeruginosa and hospital antibiotic policy prescription helps proper antimicrobial therapy and avoidance of dissemination of these multidrug resistance isolates.

Serotypes, Antibiogram and Genetic Relatedness of <i>Pseudomonas aeruginosa</i> Isolates from Urinary Tract Infections at Urology and Nephrology Center, Mansoura, Egypt

“…This was demonstrated in the present study as more than 50% of isolates were MDR. A nearby percent of MDR P. aeruginosa (43.8%) was reported by El-Domany et al [35] and a higher percent (64%) by Hashem et al [36]. Our isolates were associated with high resistance rate to piperacillin, cefoperazone/sulbactam, gentamicin and quinolones (48.7% -61.5%).…”

Phenotypic and genomic characterization of Pseudomonas aeruginosa isolates recovered from catheter-associated urinary tract infections in an Egyptian hospital

“…( b ) Previous reports for AMR found in Pseudomonas isolated from different sources in Egyptian clinical settings. Data are taken from a range of the literature [84, 86, 110–128, 129]. ESBL, extended-spectrum β-lactamase; MBL, metallo-β-lactamase.…”

Phenotypic Detection and Molecular Characterization of Metalo-Beta-lactamases in Carbapenem Resistant Pseudomonas Aeruginosa in Zagazig University Hospitals

“…The MBL positive isolates were distributed by gender, with 56.8% of the isolates being male and 43.2% female. Also, Hashem et al (13) found that the ratio of men to females was 1.8:1, with males making up 64.7% and females 35.3%. The study carried out by Sheikh et al (14) revealed very identical results, indicating that out of the 369 confirmed P. aeruginosa isolates, 219 (59.3%) isolates were acquired from male participants and 150 (40.7%) isolates from female cases.…”