Carbaryl biodegradation by Xylaria sp. BNL1 and its metabolic pathway

Li, Fēi; Lin, Dong; Liu, Yuxin; Xiao, Qiuyun; Zhang, Xiaoyu; Ma, Fuying; Yu, Hongbo

doi:10.1016/j.ecoenv.2018.10.051

Cited by 22 publications

(10 citation statements)

References 41 publications

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“…Residue of carbaryl has been detected in fresh vegetables grown in Riyadh and Asir provinces of Saudi Arabia, as well as in agri‐foods grown in China (Abdullah AlFaris et al, 2020; Fan et al, 2015; Ramadan et al, 2020). Consequently, carbaryl tends to enter the human body via food chain to induce toxicity, as also confirmed by its presence in the blood of farmers (Li et al, 2019; Soomro & Seehar, 2008). Concerning the toxicity in non‐target species, carbaryl causes histopathological, cytopathological, and ultrastructural anomalies in Oreochromis niloticus (Uğurlu et al, 2019).…”

Section: Introductionmentioning

confidence: 92%

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Saquib

Siddiqui

Ansari

et al. 2021

J of Applied Toxicology

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Pesticides have adverse effects on the cellular functionality, which may trigger myriad of health consequences. However, pesticides‐mediated toxicity in the endothelial cells (ECs) is still elusive. Hence, in this study, we have used human umbilical vein endothelial cells (HUVECs) as a model to quantify the cytotoxicity and genotoxicity of four pesticides (methomyl, carbaryl, metalaxyl, and pendimethalin). In the MTT assay, HUVECs exposed to methomyl, carbaryl, metalaxyl, and pendimethalin demonstrated significant proliferation inhibition only at higher concentrations (500 and 1000 μM). Likewise, neutral red uptake (NRU) assay also showed proliferation inhibition of HUVECs at 500 and 1000 μM by the four pesticides, confirming lysosomal fragility. HUVECs exposed to the four pesticides significantly increased the level of intracellular reactive oxygen species (ROS). Comet assay and flow cytometric data exhibited DNA damage and apoptotic cell death in HUVECs after 24 h of exposure with methomyl, metalaxyl, carbaryl, and pendimethalin. This is a first study on HUVECs signifying the cytotoxic–genotoxic and apoptotic potential of carbamate insecticides (methomyl and carbaryl), fungicide (metalaxyl), and herbicide (pendimethalin). Overall, these pesticides may affect ECs functions and angiogenesis; nonetheless, mechanistic studies are warranted from the perspective of vascular biology using in vivo test models.

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Section: Introductionmentioning

confidence: 92%

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Saquib

Siddiqui

Ansari

et al. 2021

J of Applied Toxicology

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“…It is critical to reveal key enzyme networks by designing an experiment to compare the fungal secreted proteomics between conditions with the same biomass substrate and same fungal growth stages and with only fungal extracellular enzyme profile changes, which could result in changes in biomass degradation profiles. It was found that the addition of manganese ions (Mn 2+ ) can improve the efficiency of white‐rot fungal biomass degradation by altering the extracellular enzyme expression profiles (Salvachúa et al ., 2013; Song et al ., 2013), especially auxiliary active family proteins, including AA1, AA2 and possibly LPMO (Jung et al ., 2015; Li et al ., 2019a). Manganese ions could affect the expression or activity of these enzymes by regulating the upstream expression element of the gene or changing the active sites as metal auxiliary elements (Stajic et al ., 2006; Zhuo et al ., 2017).…”

Section: Resultsmentioning

confidence: 99%

Lytic polysaccharide monooxygenases promote oxidative cleavage of lignin and lignin–carbohydrate complexes during fungal degradation of lignocellulose

Zhang

et al. 2021

Environmental Microbiology

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Summary Overcoming lignocellulosic biomass recalcitrance, especially the cleavage of cross‐linkages in lignin–carbohydrate complexes (LCCs) and lignin, is essential for both the carbon cycle and industrial biorefinery. Lytic polysaccharide monooxygenases (LPMOs) are copper‐containing enzymes that play a key role in fungal polysaccharide oxidative degradation. Nevertheless, comprehensive analysis showed that LPMOs from a white‐rot fungus, Pleurotus ostreatus, correlated well with the Fenton reaction and were involved in the degradation of recalcitrant nonpolysaccharide fractions in this research. Thus, LPMOs participated in the extracellular Fenton reaction by enhancing iron reduction in quinone redox cycling. A Fenton reaction system consisting of LPMOs, hydroquinone, and ferric iron can efficiently produce hydroxy radicals and then cleave LCCs or lignin linkages. This finding indicates that LPMOs are underestimated auxiliary enzymes in eliminating biomass recalcitrance.

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“…The quinones formed may be used as substrates for laccase and other peroxidases. Laccase and CYP450 break the ring of 1,4 naphthoquinone, forming benzoic acid, which may be further metabolized into CO 2 and water ( [224] (Figure 5).…”

Section: Mechanism Of Heavy Metal and Pesticide Remediation By Microo...mentioning

confidence: 99%

Microbial Remediation: A Promising Tool for Reclamation of Contaminated Sites with Special Emphasis on Heavy Metal and Pesticide Pollution: A Review

et al. 2022

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Heavy metal and pesticide pollution have become an inevitable part of the modern industrialized environment that find their way into all ecosystems. Because of their persistent nature, recalcitrance, high toxicity and biological enrichment, metal and pesticide pollution has threatened the stability of the environment as well as the health of living beings. Due to the environmental persistence of heavy metals and pesticides, they get accumulated in the environs and consequently lead to food chain contamination. Therefore, remediation of heavy metals and pesticide contaminations needs to be addressed as a high priority. Various physico-chemical approaches have been employed for this purpose, but they have significant drawbacks such as high expenses, high labor, alteration in soil properties, disruption of native soil microflora and generation of toxic by-products. Researchers worldwide are focusing on bioremediation strategies to overcome this multifaceted problem, i.e., the removal, immobilization and detoxification of pesticides and heavy metals, in the most efficient and cost-effective ways. For a period of millions of evolutionary years, microorganisms have become resistant to intoxicants and have developed the capability to remediate heavy metal ions and pesticides, and as a result, they have helped in the restoration of the natural state of degraded environs with long term environmental benefits. Keeping in view the environmental and health concerns imposed by heavy metals and pesticides in our society, we aimed to present a generalized picture of the bioremediation capacity of microorganisms. We explore the use of bacteria, fungi, algae and genetically engineered microbes for the remediation of both metals and pesticides. This review summarizes the major detoxification pathways and bioremediation technologies; in addition to that, a brief account is given of molecular approaches such as systemic biology, gene editing and omics that have enhanced the bioremediation process and widened its microbiological techniques toward the remediation of heavy metals and pesticides.

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Carbaryl biodegradation by Xylaria sp. BNL1 and its metabolic pathway

Cited by 22 publications

References 41 publications

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Cytotoxicity and genotoxicity of methomyl, carbaryl, metalaxyl, and pendimethalin in human umbilical vein endothelial cells

Lytic polysaccharide monooxygenases promote oxidative cleavage of lignin and lignin–carbohydrate complexes during fungal degradation of lignocellulose

Microbial Remediation: A Promising Tool for Reclamation of Contaminated Sites with Special Emphasis on Heavy Metal and Pesticide Pollution: A Review

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