Carbohydrate epitopes as a cause of cross-reactivity in patients allergic to Hymenoptera venom

Eržen, Renato; Korošec, Peter; Šilar, Mira; Mušič, Ema; Košnik, Mitja

doi:10.1007/s00508-009-1171-1

Cited by 17 publications

(20 citation statements)

References 13 publications

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“…This correlates with earlier studies which showed a prevalence of 56–80.5% [5,8,13]. Interestingly, Eržen et al [14] found a lower prevalence of about 40% for CCD-sIgE.…”

Section: Discussionsupporting

confidence: 79%

“…Several studies have shown that the major cause for this phenomenon is most likely the presence of sIgE directed against carbohydrate epitopes (CCDs) [5,8,14]. Therefore, determination of CCD-sIgE levels in patients with venom double-positive sIgE should be performed.…”

Section: Discussionmentioning

confidence: 99%

“…To date, for detection of CCD-sIgE different glycoprotein-containing extracts and single glycoproteins are used. The most commonly used ones are oilseed rape and natural rubber latex extracts, ascorbic acid oxidase (AAO), bromelain, and horseradish peroxidase (HRP) [5,8,13,14,16,18,19]. Recently, isolated N -glycan chains from bromelain (structure MUXF 3 ; fig.…”

Section: Introductionmentioning

confidence: 99%

“…One major problem in diagnosing hymenoptera venom allergy is the fact that up to 60% of patients’ sera show in vitro reactivity to both yellow jacket venom (YJV) and honeybee venom (HBV) [5,7,8,9,10]. Causes for this phenomenon may be true, independent co-sensitizations or cross-reactions between YJV and HBV allergens, either on the basis of sequence homology, for example between HB- and YJ-hyaluronidases [11,12], or, predominantly, due to the presence of sIgE directed against cross-reactive carbohydrate determinants (CCDs) [5,8,13,14]. Whereas true double sensitization would be treated by immunotherapy with both venoms, in the case of double positivity due to cross-reactions, SIT with the primarily responsible venom alone would be sufficient [3].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, isolated N -glycan chains from bromelain (structure MUXF 3 ; fig. 1) were also introduced for detection of CCD-sIgE [5,14,18]. Information about carbohydrate structures and number of carbohydrate chains of the glycoproteins analyzed is given in figure 1.…”

Section: Introductionmentioning

confidence: 99%

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Suitability of Different Glycoproteins and Test Systems for Detecting Cross-Reactive Carbohydrate Determinant-Specific IgE in Hymenoptera Venom-Allergic Patients

Mertens

2011

Int Arch Allergy Immunol

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Background: In hymenoptera venom allergy, about 75% of detected in vitro double positivity to yellow jacket and honeybee venom is ascribed to specific IgE (sIgE) directed against cross-reactive carbohydrate determinants (CCDs). To date, for the detection of CCD-sIgE, different carbohydrate antigens and methods are used. The most suitable one still has to be identified. Methods: Eighty-seven patients with confirmed hymenoptera venom allergy and venom sIgE values of ≧0.7 kU/l were investigated. Sixty-five patients showed sIgE reactivity to both yellow jacket and honeybee venom, 22 were venom mono positive and served as controls. Occurrence of CCD-sIgE was determined using bromelain, horseradish peroxidase (HRP) and MUXF³ on system A, and ascorbic acid oxidase (AAO), bromelain and HRP on system B. Further, a reference standard for CCD-sIgE evaluation was created: CCD positivity was assumed when at least 4 of the 6 test results were positive. Results: According to the defined reference standard, 45/65 venom double positive patients exhibited CCD-sIgE. Using system A, comparison with the reference standard revealed sensitivity and specificity values of 96 and 97%, respectively, for MUXF³, 100 and 100%, respectively, for bromelain, and 96 and 97%, respectively, for HRP. Using system B, sensitivity and specificity was 98 and 97%, respectively, for AAO, 62 and 95%, respectively, for bromelain, and 96 and 69%, respectively, for HRP. Results of the 3 test allergens obtained with system A showed strong correlations (r = 0.932–0.976), whereas results with system B showed lower correlations (r = 0.714–0.898). Conclusions: All 3 test allergens used with system A are suitable for CCD-sIgE detection in hymenoptera venom allergy. With system B, only AAO seems to be a reliable tool.

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“…This correlates with earlier studies which showed a prevalence of 56–80.5% [5,8,13]. Interestingly, Eržen et al [14] found a lower prevalence of about 40% for CCD-sIgE.…”

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Suitability of Different Glycoproteins and Test Systems for Detecting Cross-Reactive Carbohydrate Determinant-Specific IgE in Hymenoptera Venom-Allergic Patients

Mertens

2011

Int Arch Allergy Immunol

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Hymenoptera-Induced Hypersensitivity Reactions and Anaphylaxis

Košnik

Korošec²

2010

Anaphylaxis and Hypersensitivity Reactions

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COPD is not COPD is not ALLERGY

Šuškovič

2009

Wien Klin Wochenschr

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Chronic obstructive pulmonary disease (COPD) is still a poorly understood disease. Its pathogenesis is excitingly complex and has systemic consequences caused not only by increased production of certain cytokines but also by neurohumoral activation, chronic bacterial infection, muscle wasting and cachexia. Asthma and COPD have many overlapping clinical features so it should not be surprising that in the pathogenesis of COPD mediators such as leukotrienes, complement activation, atopic or even autoimmune processes are possibly involved. The pathogenesis of cardiovascular system involvement in COPD is also multifaceted and includes chronic heart hypoxia, damage by smoking and pulmonary hypertension; it must also be viewed as a consequence of systemic inflammation and neurohormonal activation. COPD is among the leading causes of morbidity and mortality worldwide and therefore it should be studied intensively beyond the lung itself. Treatments directed at neurohumoral activation in COPD have not been fully addressed; this aspect of COPD should be better understood, as it may direct novel therapeutic approaches.

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Carbohydrate epitopes as a cause of cross-reactivity in patients allergic to Hymenoptera venom

Cited by 17 publications

References 13 publications

Suitability of Different Glycoproteins and Test Systems for Detecting Cross-Reactive Carbohydrate Determinant-Specific IgE in Hymenoptera Venom-Allergic Patients

Suitability of Different Glycoproteins and Test Systems for Detecting Cross-Reactive Carbohydrate Determinant-Specific IgE in Hymenoptera Venom-Allergic Patients

Hymenoptera-Induced Hypersensitivity Reactions and Anaphylaxis

COPD is not COPD is not ALLERGY

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