1996
DOI: 10.1093/jxb/47.2.161
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Carbohydrate metabolism in the developing and maturing wheat embryo in relation to its desiccation tolerance

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Cited by 106 publications
(91 citation statements)
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“…According to Koster and Leopold 1988;Leprince et al 1993;Black et al 1996;Brenac et al 1997, RFO are being accumulated in maturing seeds of many species, and there is a positive correlation between the level of these compounds and seed longevity (Bernal-Lugo and Leopold 1992;Bernal-Lugo and Leopold 1995). The ratio of seed sucrose/raffinose family oligosaccharides (S/RFO) seems particularly relevant.…”
Section: Discussionmentioning
confidence: 99%
“…According to Koster and Leopold 1988;Leprince et al 1993;Black et al 1996;Brenac et al 1997, RFO are being accumulated in maturing seeds of many species, and there is a positive correlation between the level of these compounds and seed longevity (Bernal-Lugo and Leopold 1992;Bernal-Lugo and Leopold 1995). The ratio of seed sucrose/raffinose family oligosaccharides (S/RFO) seems particularly relevant.…”
Section: Discussionmentioning
confidence: 99%
“…Root tissue carbohydrates were extracted using a methodology adapted from Black et al [52]; 200 mg samples were homogenized in 1 mL of 80% (v/v) ethyl alcohol and incubated at 75 • C for 30 min, then centrifuged at 10,000 rpm for five minutes at 10 • C. This procedure was repeated two more times. After extraction, supernatants and precipitate were oven dried at 45 • C for 48 h. Residual supernatant was resuspended in 1 mL distilled water for TSS analysis.…”
Section: Field Sampling and Laboratory Methodsmentioning
confidence: 99%
“…α-amylase activity was measured according to Black et al (1996). Seeds were homogenized in an Eppendorf tube with 4 ml ice-cold 20 mM TRISmaleate buffer (pH 6.2) containing 1 mM CaCl 2 .…”
Section: Germination Testsmentioning
confidence: 99%