Carbohydrate Source, Biomass Productivity and Natural Product Yield in Cell Suspension Cultures

Fowler, Michael; Stepan-Sarkissian, G.

doi:10.1007/978-3-642-70717-9_6

Cited by 19 publications

(4 citation statements)

References 15 publications

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“…after three weeks of culture. In all situations the uptake of glucose dominates fructose uptake (Table 2) which is in agreement with results reported for other culture systems (Fowler and Sarkissian, 1985). However, as the data in Table 3 will show the distribution of ~4C coming from either hexose follows at least qualitatively the same pattern.…”

Section: Resultssupporting

confidence: 90%

“…Some reports also point to influences of light and possibly photosynthesis, as well as the kind of carbohydrate in the nutrient medium, on the production of secondary products in cultured cells (for review see Fowler and Sarkissian, 1985;Luckner and Dietterich, 1985; Barz and Hiisemann, 1982). In most cell culture systems sucrose is the preferred carbohydrate to supply carbon and energy.…”

Section: Introductionmentioning

confidence: 99%

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On carbohydrate metabolism of cultured carrot root explants

Bender

Pauler

Neumann

1987

Plant Cell Tiss Organ Cult

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

On carbohydrate metabolism of cultured carrot root explants

Bender

Pauler

Neumann

1987

Plant Cell Tiss Organ Cult

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“…For many types of cell suspensions such an extracellular hydrolysis of sucrose already has been described (Fowler & Stepan-Sarkissan 1985, Callebaut et al 1987, Dijkema et al 1988). Such a lack of substrates might manifest itself in changes in the sugar pools and in the sugar metabolism.…”

Section: Discussionmentioning

confidence: 96%

Accumulation of anthraquinones in Morinda citrifolia cell suspensions

Hagendoorn¹,

Plas²,

Segers³

1994

Plant Cell Tiss Organ Cult

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Cell suspensions of Morinda citrifolia were cultivated in a B5-medium containing 4% sucrose as the sole carbon source and 1 mg 1-1 naphthyl acetic acid (NAA) or 1 mg 1-1 2,4-dichloro-phenoxyacetic acid (2,4-D). Both auxins were able to support growth but only in the presence of NAA anthraquinone production was observed. 2,4-D inhibited the production in NAA cultures. Anthraquinone synthesis took place in the growth and the stationary phase and amounts of 0.2--43.4 mmol (about 100-200 mg) g-1 dry weight could be reached.Under both growth conditions sucrose was hydrolyzed extracellularly by invertase. From the resulting monosaccharides, glucose was taken up preferentially and an appreciable uptake of fructose only took place when medium glucose was exhausted. Sugar uptake rates were similar when cells were grown in NAA and in 2,4-D medium but the intracellular sugar contents (expressed on a dry weight basis) differed considerably. The presence of sucrose, glucose and fructose was demonstrated under both growth conditions. The amounts of sucrose and glucose were much lower in the 2,4-D cells than in the NAA-cells especially during the growth phase. Fructose contents were low and comparable, while in NAA cells an unknown sugar (possibly the sugar moiety of the glycosylated anthraquinones) was observed especially at the end of the growth phase and in the stationary phase. The differences in sugar concentrations were even larger due to the lower water contents of the NAA cells.Respiration of 2,4-D cells was much higher than that of NAA cells during the growth phase. A sharp increase in sugar contents (mainly sucrose) occurred in the 2,4-D cells at the end of the growth phase and corresponded with the fall in respiratory activity.A possible correlation between the lack of production of anthraquinones in 2,4-D cells and a less efficient growth metabolism in these cells is discussed.Abbreviations: AQ -anthraquinones, 2,4-D -2,4-dichloro-phenoxy-acetic acid, DW -dry weight, FW -fresh weight, NAA -, naphthyl acetic acid, pCPO -p-chloro-phenoxy-acetic acid

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“…In all cases the glucose produced is clearly used preferentially to the fructose and depleted before the fructose. Some aspects of the utilization of sucrose have been discussed by Fowler & Stephens-Sarkisian [12] because in a number of cultures, sucrose gives higher yield of phytochemicals than does glucose, and by Sung et al [25] because of new information on sucrose metabolism in plants.…”

Section: Discussionmentioning

confidence: 99%

Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells

Dougall

Frazier

1989

Plant Cell Tiss Organ Cult

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The medium used for the growth of anthocyanin-accumulating wild carrot (D. carota) suspension cultures contained ammonia as a sole nitrogen source and was buffered with succinate. Ammonia was the first nutrient to be completely utilized.The uptake of carbohydrate, phosphate and succinate continued after ammonia depletion. Biomass accumulation was faster and greater when sucrose was initially present in the medium than when glucose was present. When sucrose was provided in the medium it was rapidly hydrolysed to glucose and fructose and the fructose was used preferentially to glucose. Anthocyanin accumulation was rapid after ammonia fell below 3 mM and until the pH of the medium rose from 4.5 to 5.1 or 5.2.

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Carbohydrate Source, Biomass Productivity and Natural Product Yield in Cell Suspension Cultures

Cited by 19 publications

References 15 publications

On carbohydrate metabolism of cultured carrot root explants

On carbohydrate metabolism of cultured carrot root explants

Accumulation of anthraquinones in Morinda citrifolia cell suspensions

Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells

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