Carbohydrate Sulfotransferases

Rosen, Steven D.; Bistrup, Annette; Hemmerich, Stefan

doi:10.1002/9783527618255.ch53

Cited by 4 publications

(7 citation statements)

References 89 publications

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“…The predicted protein sequence of GST-5 contains the same three regions of high sequence conservation (50 -95%) with other members of the GST family that have been identified previously (4,16,18,26). Two of these regions likely constitute the binding site for the universal sulfate donor PAPS (31), while the functional relevance of the most C-terminal consensus region remains to be determined.…”

Section: Fig 3 Northern Blotting Northern Blots Containing Poly(a)mentioning

confidence: 99%

“…After concentration and dilution in CH 2 Cl 2 , the or-ganic layer was washed with saturated NaHCO 3 , water, and brine. The organic layer was dried over Mg 2 SO 4 …”

Section: Octyl 346-tri-o-acetyl-2-acetamido-2-deoxy-␤-d-glucopyranomentioning

confidence: 99%

“…We have previously used this IgG chimera of GlyCAM-1 to characterize the sulfotransferase activities of GST-1, -2, -3, and -4 (16,18). 4 The transfected COS-7 cells were cultured in the presence of [ 35 S]sulfate. GlyCAM-1/IgG was purified from the conditioned medium on protein A-agarose and analyzed by SDS-PAGE and autoradiography.…”

Section: Identification Of An Open Reading Frame Encoding a Novel Galmentioning

confidence: 99%

“…As sulfate modifications can be extensive and tend to occur in clusters in glycoproteins, they can have a profound effect on the physicochemical character of the glycoconjugate, at least in part by conferring a negative charge at physiological pH (3). In addition, regioselective sulfation of carbohydrates frequently generates specific epitopes that can be recognized by hormones, extracellular matrix proteins, cell surface receptors, and viruses (2)(3)(4).…”

mentioning

confidence: 99%

“…6-O-Sulfation on galactose and N-acetylglucosamine (i.e. GlcNAc-6-SO 4 and Gal-6-SO 4 ) which is found in the context of sialyl Lewis X, is necessary for high affinity binding between Lselectin and these HEV-associated ligands (9).…”

mentioning

confidence: 99%

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Sulfation of N-Acetylglucosamine by Chondroitin 6-Sulfotransferase 2 (GST-5)

Bhakta¹,

Bartes²,

Bowman³

et al. 2000

Journal of Biological Chemistry

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Section: Fig 3 Northern Blotting Northern Blots Containing Poly(a)mentioning

confidence: 99%

“…After concentration and dilution in CH 2 Cl 2 , the or-ganic layer was washed with saturated NaHCO 3 , water, and brine. The organic layer was dried over Mg 2 SO 4 …”

Section: Octyl 346-tri-o-acetyl-2-acetamido-2-deoxy-␤-d-glucopyranomentioning

confidence: 99%

Section: Identification Of An Open Reading Frame Encoding a Novel Galmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Sulfation of N-Acetylglucosamine by Chondroitin 6-Sulfotransferase 2 (GST-5)

Bhakta¹,

Bartes²,

Bowman³

et al. 2000

Journal of Biological Chemistry

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High Endothelial Cell N-Acetylglucosamine 6-O-Sulfotransferase

Bistrup

Rosen

2002

Handbook of Glycosyltransferases and Related Genes

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Core structures of polysialylated glycans present in neural cell adhesion molecule from newborn mouse brain

Geyer

Bahr

Liedtke

et al. 2001

European Journal of Biochemistry

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Polysialylation of the neural cell adhesion molecule (N-CAM) is known to destabilize cell-cell adhesion and to promote plasticity in cell -cell interactions. To gain more insights into the molecular mechanisms regulating the selective expression of polysialic acid on distinct glycan chains, the underlying core structures of polysialylated N-CAM glycans from newborn mouse brain were examined. Starting from low picomolar amounts of oligosaccharides, a multistep approach was used that was based on various mass spectrometric techniques with minimized sample consumption. Evidence could be provided that polysialylated murine N-CAM glycans comprise diantennary, triantennary and tetraantennary core structures carrying, in part, type-1 N-acetyllactosamine antennae, sulfate groups linked to terminal galactose or subterminal N-acetylglucosamine residues and, as a characteristic feature, a sulfated glucuronic acid unit which was bound exclusively to C3 of terminal galactose in Mana3-linked type-2 antennae. Hence, our results reveal that part of the murine N-CAM carbohydrates are modified within a single oligosaccharide by polysialic acid plus a HSO 3 -GlcA-moiety, which is likely to represent a HNK1-epitope. As HNK1-carbohydrates are also known to modulate cell -cell interactions, the simultaneous presence of both carbohydrate epitopes may reflect a new mechanism involved in the fine-tuning of N-CAM functions.

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Carbohydrate Sulfotransferases

Cited by 4 publications

References 89 publications

Sulfation of N-Acetylglucosamine by Chondroitin 6-Sulfotransferase 2 (GST-5)

Sulfation of N-Acetylglucosamine by Chondroitin 6-Sulfotransferase 2 (GST-5)

High Endothelial Cell N-Acetylglucosamine 6-O-Sulfotransferase

Core structures of polysialylated glycans present in neural cell adhesion molecule from newborn mouse brain

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