Carbon nanofiber-based composites for the construction of mediator-free biosensors

Myoglobin/gold nanoparticles/carbon spheres 3-D architecture for the fabrication of a novel biosensor

“…Extensive work has been carried out with redox protein films over the past few decades in order to find methods to increase the electron transfer rate between enzyme and electrode [1 8]. For example, a variety of carbonbased materials such as carbon nanotubes (CNTs) [9 11] and carbon nanofi bers (CNFs) [12,13] have been successfully employed for the direct electron transfer of proteins in fi lms. Their properties of hydrophilicity, nontoxicity, excellent film-forming ability, and remarkable biocompatibility offer excellent prospects for biocompatible immobilization matrices and the design of novel biosensors.…”

Improved Direct Electrochemistry for Proteins Adsorbed on a UV/Ozone-Treated Carbon Nanofiber Electrode

“…The CNF was modified by the reaction of liquid-phase molecules containing an alkene group induced by irradiating ultraviolet light for around 16 h. 25 Li's group also obtained the DET of hemoglobin (Hb) by using a hydrophilic organic-inorganic composite consisting of CNFs and Nafion, and then developed a Hb-based biosensor. 24 These previous reports suggest that hydrophilic CNFs with a sufficient number of functionalized surface groups are very significant for achieving DET. It has been reported that the surface oxygen functional groups of carbon nanostructures play an important role in both improving wettability and facilitating DET with enzymes, such as bilirubin oxidase (BOD).…”

“…This is because we first need to create open ends (or defect sites) at the closed sidewalls of the CNTs to which we can attach functional groups for further functionalization. 22,23 In contrast, CNFs are lessordered materials and have more edge planes on their sidewalls, 23,24 which may result in more facile electron transfer than CNTs. This also indicates that the CNF sidewalls are more reactive and the surface structure is easier to functionalize by using various chemical modifications.…”

Non-covalent functionalization of CVD-grown graphene with Au nanoparticles for electrochemical sensing application

“…In literature, highly sensitive and selective enzymes have been recommended as potential candidates to detect H 2 O 2 [28][29][30][31]. In 2010, Zhou et al [32] demonstrated a novel H 2 O 2 biosensor, co-immobilizing horseradish peroxidase (HRP) with graphene into biocompatible polymer chitosan, with a detection limit of 1.7 lM within a board linear range from 0.005 to 5.13 mM.…”