Carbon Nanotubes in Biomedical Applications: Factors, Mechanisms, and Remedies of Toxicity

Alshehri, Reem A; Ilyas, Asad Muhammad; Hasan, Anwarul; Arnaout, Adnan; Ahmed, Farid; Memić, Adnan

doi:10.1021/acs.jmedchem.5b01770

Cited by 337 publications

(216 citation statements)

References 211 publications

(348 reference statements)

Supporting

Mentioning

214

Contrasting

Order By: Relevance

“…Recently, multiwalled carbon nanotubes were used as carriers for the cellular delivery of conjugated pHK (61). Although useful as drug delivery platforms, carbon nanotubes currently suffer from several limitations, including significant intrinsic cytotoxicity and variability in size, morphology, and purity, as well as poor drug loading, retention, or release (62, 63).…”

Section: Discussionmentioning

confidence: 99%

“…Our results are in line with a recent study that showed that delivery of the N‐terminal 15 aa of HKII by multiwalled carbon nanotubes resulted in substantially greater toxicity in MCF‐7 human breast cancer cells and HCT116 human colon carcinoma cells compared with noncancerous IMR‐90 human fetal lung fibroblasts (61). It should be noted that significant toxicity was also reported in IMR‐90 cells, which is likely the result of the inherent cytotoxicity of the carbon nanotube delivery platform (62, 63). Here, the small apparent decrease in MTS response of HEK293 cells that were treated with higher peptide concentrations of pHK‐PAS (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hexokinase II–derived cell‐penetrating peptide targets mitochondria and triggers apoptosis in cancer cells

et al. 2017

View full text Add to dashboard Cite

Overexpression of mitochondria-bound hexokinase II (HKII) in cancer cells plays an important role in their metabolic reprogramming and protects them against apoptosis, thereby facilitating their growth and proliferation. Here, we show that covalently coupling a peptide corresponding to the mitochondrial membrane–binding N-terminal 15 aa of HKII (pHK) to a short, penetration-accelerating sequence (PAS) enhances the cellular uptake, mitochondrial localization, and cytotoxicity of the peptide in HeLa cells. Further analysis revealed that pHK-PAS depolarized mitochondrial membrane potential, inhibited mitochondrial respiration and glycolysis, and depleted intracellular ATP levels. The effects of pHK-PAS were correlated with dissociation of endogenous full-length HKII from mitochondria and release of cytochrome c. Of significance, pHK-PAS treatment of noncancerous HEK293 cells resulted in substantially lower cytotoxicity. Thus, pHK-PAS effectively disrupted the mitochondria-HKII association in cancer cells, which led to mitochondrial dysfunction and, finally, apoptosis. Our results demonstrate the potential of the pHK-PAS cell-penetrating peptide as a novel therapeutic strategy in cancer.—Woldetsadik, A. D., Vogel, M. C., Rabeh, W. M., Magzoub, M. Hexokinase II–derived cell-penetrating peptide targets mitochondria and triggers apoptosis in cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hexokinase II–derived cell‐penetrating peptide targets mitochondria and triggers apoptosis in cancer cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…97,98 Consequently, suitably altered functionalised CNTs are attractive candidates for biomedical applications as well as material development approaches. [99][100][101][102] Inherent hydrogen bond-mediated assembly of nucleobases provided the impetus to explore self-assembly of guanine-single walled CNT hybrids on HOPG surface. Covalently functionalised guanine-SWCNT (SWNT: single walled carbon nanotubes) 1 appeared as small bundles on TEM analysis, which in the presence of K + ions (4 : 1), indirectly revealed a G-quartet type of self-assembled pattern on HOPG/mica surface (Fig.…”

Section: Surface Modification By Nucleobase-cnt Hybridsmentioning

confidence: 99%

Surface modification and pattern formation by nucleobases and their coordination complexes

et al. 2018

View full text Add to dashboard Cite

This review presents recent progress concerning the organization of nucleobases on highly ordered pyrolytic graphite (HOPG), mica, Cu(110) and Au(111) surfaces, followed by their studies using microscopy methods such as atomic force microscopy (AFM), scanning tunneling microscopy (STM) and transmission electron microscopy (TEM). Interesting research prospects related to surface patterning by nucleobases, nucleobase-functionalized carbon nanotubes (CNTs) and metal-nucleobase coordination polymers are also discussed, which offer a wide array of functional molecules for advanced applications. Nucleobases and their analogs are able to invoke non-covalent interactions such as p-p stacking and hydrogen bonding, and possess the required framework to coordinate metal ions, giving rise to fascinating supramolecular architectures. The latter could be transferred to conductive substrates, such as HOPG and gold, for assessment by high-end tunneling microscopy under various conditions. Clear understanding of the principles governing nucleobase self-assembly and metal ion complexation, and precise control over generation of functional architectures, might lead to custom assemblies for targeted nanotechnological and nanomaterial applications.

show abstract

“…It is commonly accepted that pristine CNTs (p‐CNTs) are cytotoxic and cause inflammation to human organs. However, the modification of nanotube surfaces alters their behavior towards biological systems and reduces their toxicity . Grafting dendrimers on CNTs surfaces enables such a phenomenon .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Catalytic Applications of Multi‐Walled Carbon Nanotube–Polyamidoamine Dendrimer Hybrids

Desmecht

Steenhaut

Pennetreau

et al. 2018

Chemistry A European J

View full text Add to dashboard Cite

Polyamidoamine (PAMAM) dendrimers were covalently immobilized on multi-walled carbon nanotubes (MWNT) by two "grafting to" strategies. We demonstrate the existence of non-covalent interactions between the two components but outline the superiority of our two grafting approaches, namely xanthate and click chemistry. MWNT surfaces were functionalized with activated ester and propargylic moieties prior to their reaction with PAMAM or azido-PAMAM dendrimers, respectively. The grafting of PAMAM generations 0 to 3 was evaluated with X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and transmission electron microscopy (TEM). The versatility of our hybrids was demonstrated by post-functionalization sequences involving copper alkyne-azide cycloaddition (CuAAC). We synthesized homogeneous supported iridium complexes at the extremities of the dendrimers. In addition, our materials were used as templates for the encapsulation of Pd nanoparticles (NPs), validating our nanocomposites for catalytic applications. The palladium-based catalyst was active for carbonylative coupling over five consecutive runs without loss of activity.

show abstract

Carbon Nanotubes in Biomedical Applications: Factors, Mechanisms, and Remedies of Toxicity

Cited by 337 publications

References 211 publications

Hexokinase II–derived cell‐penetrating peptide targets mitochondria and triggers apoptosis in cancer cells

Hexokinase II–derived cell‐penetrating peptide targets mitochondria and triggers apoptosis in cancer cells

Surface modification and pattern formation by nucleobases and their coordination complexes

Synthesis and Catalytic Applications of Multi‐Walled Carbon Nanotube–Polyamidoamine Dendrimer Hybrids

Contact Info

Product

Resources

About