Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5

Hwang, Soonho; Kang, Joong Koo; Kim, Min Jae; Shin, Dong Min; Hong, Jeong Hee

doi:10.1080/14756366.2018.1540475

Cited by 10 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, AQP3 and AQP4 have been associated with normal physiological processes in skin hydration and CNS, respectively. Human patients carrying E143K mutation in carbonic anhydrase showed a dry mouth syndrome due to mislocalization of AQP5 in the salivary glands [30]. Collectively, mouse KO and other studies have shown a critical role of AQPs in several normal physiological processes in extrapulmonary organs [reviewed in [6].…”

Section: Extrapulmonary Rolementioning

confidence: 99%

Aquaporins in lung health and disease: Emerging roles, regulation, and clinical implications

Yadav

Hus

et al. 2020

Respiratory Medicine

View full text Add to dashboard Cite

Section: Extrapulmonary Rolementioning

confidence: 99%

Aquaporins in lung health and disease: Emerging roles, regulation, and clinical implications

Yadav

Hus

et al. 2020

Respiratory Medicine

View full text Add to dashboard Cite

“…This was explained by a possible theory that CA12 (E143K) mutation generates an acidic environment intracellularly, and this low pH may affect AQP5, which are considered pH sensitive. [Hong et al, 2015;Hwang et al, 2019]. Further investigation of this association is needed to possibly understand the complex mechanisms of dental erosions development.…”

Section: Discussionmentioning

confidence: 99%

Aquaporins’ Influence on Different Dental Erosive Wear Phenotypes in Humans

et al. 2020

View full text Add to dashboard Cite

Dental erosive wear is a multifactorial condition of high prevalence. Nowadays, there is an emphasis on discovering individual genetic predisposition for the development of this condition. Aquaporins (AQPs) are water channel proteins expressed in salivary glands, as well as during tooth development. They are involved in salivary secretion and composition and linked to physiological protection of the oral cavity. The aim of this study was to explore the relationship between different dental erosive wear phenotypes, AQP genes, and selected environmental factors. Data from 705 dental patients were used to investigate the association between dental erosive wear phenotypes and AQPs' single-nucleotide variants. Phenotypes were further analyzed considering diet and oral hygiene data, using logistic regression analysis, as implemented in PLINK, with the assumption that dental erosive wear is a complex gene-environment model. Associations were found between severe erosive tooth wear and rs2878771 (AQP2) for the genotypic (p = 0.02) and dominant (p = 0.03) models, and rs3736309 (AQP5) for the allelic model (p = 0.02). Logistic regression analyses, after implementing the Bonferroni correction, showed that several significant associations were present when covariates were included, suggesting that a strong environmental component is present. Our results show that dental erosive wear establishes under a gene-environmental complex model.

show abstract

“…The calcein-AM was excited at 495 nm, and the emitted fluorescence was measured at 515 nm. The volume of FLS or HEK293T cells was determined using a hypertonic (400 mOsm/L, Table 1 ) solution ( Hwang et al, 2019 ). Fluorescence images were automatically captured at 1-s intervals using a CCD camera (Q-Imaging, British Columbia, Canada, Retiga 6000) linked to an inverted microscope (Olympus, Tokyo, Japan) and analyzed with the MetaFluor system (Molecular Devices).…”

Section: Methodsmentioning

confidence: 99%

Synovial Fluid of Patient With Rheumatoid Arthritis Enhanced Osmotic Sensitivity Through the Cytotoxic Edema Module in Synoviocytes

Ryu

Hong

2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is an autoimmune disease that causes inflammation of the synovial membrane ultimately leading to permanent damage in the affected joints. For this study, synovial fluids from 16 patients diagnosed with either RA or osteoarthritis (OA) were used to examine volume regulation and cooperative water channels, both of which are involved in the cytotoxic edema identified in RA-fibroblast-like synoviocytes (FLS). The osmolarity and inflammatory cytokine interleukin (IL)-6 of synovial fluids from RA patients were mildly enhanced compared to that from OA patients. RA-FLS demonstrated the enhanced property of regulatory volume increase in response to IL-6 and synovial fluids from RA patients. Although there was no difference in the protein expression of the volume-associated protein sodium–potassium–chloride cotransporter1 (NKCC1), its activity was increased by treatment with IL-6. Membrane localization of NKCC1 was also increased by IL-6 treatment. Additionally, both the protein and membrane expressions of aquaporin-1 were increased in RA-FLS by IL-6 stimulation. The IL-6-mediated enhanced osmotic sensitivity of RA-FLS likely involves NKCC1 and aquaporin-1, which mainly constitute the volume-associated ion transporter and water channel elements. These results suggest that RA-FLS provide enhanced electrolytes and concomitant water movement through NKCC1 and aquaporin-1, thereby inducing cellular swelling ultimately resulting in cytotoxic edema. Attenuation of cytotoxic edema and verification of its related mechanism will provide novel therapeutic approaches to RA treatment within the scope of cytotoxic edema.

show abstract

Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5

Cited by 10 publications

References 29 publications

Aquaporins in lung health and disease: Emerging roles, regulation, and clinical implications

Aquaporins in lung health and disease: Emerging roles, regulation, and clinical implications

Aquaporins’ Influence on Different Dental Erosive Wear Phenotypes in Humans

Synovial Fluid of Patient With Rheumatoid Arthritis Enhanced Osmotic Sensitivity Through the Cytotoxic Edema Module in Synoviocytes

Contact Info

Product

Resources

About