Carbonic anhydrase activity is increased in retinal pigmented epithelium and choriocapillaris of RCS rats

Abstract

Changes in CA activity in the RPE and adjacent capillary endothelium, together with previously described changes in RPE morphology in RCS rats, indicate changes in ion and fluid transport across the RPE. Since the retina was already impaired when the increase in CA activity occurred, we hypothesize that the causative factor is not the genetic defect per se, but the destruction of the retina.

Comparative mode-of-action analysis following manual and automated phenotype detection inXenopus laevis

“…58 Regarding the remaining two targets for which predicted activity has been classied as causing the melanophore migration phenotype, carbonic anhydrase (CA) 6 and cytochrome P450 1A2, both may have indirect involvement in the phenotype. In particular, CA activity has been shown to be increased in the retinal pigmented epithelium in rat, 59 whereas another CA member, CA9, has been associated with cell migration. 60 Finally, for cytochrome P450 1A2, we cannot infer a direct relationship in the mechanism of action of these compounds but there may be involvement in metabolism.…”

Retinochoroidal anastomoses in RCS rat eyes?

“…After contact with the RPE the retinal capillaries become fenestrated [15,16], show increased permeability for horseradish peroxydase [17,18] and increase in carbonic anhydrase activity [19]. In the process of retinal degeneration, the morphology of the retinal capillary network changes considerably.…”

Okuläre Pulsamplitude, intraokulärer Druck und Betablocker/Karboanhydrasehemmung in der Kombinationstherapie beim primären Offenwinkelglaukom

“…Die bis heute bekannten sieben Isoenzyme, von denen zwei Transport von HCO3 und Natrium (Na), seinern korrespondierenden Kation -HCO3 und Na benutzen denselben Transportmechanisrnus -, und steigert so den Transport von Wasser in das Karnmerwasser. Die Karboanhydrase wurde in den Arterien des Gehirns[41] und den Kapillaren von Netz-und Aderhaut [44], insbesondere der Choriocapillaris[31], nachgewiesen. Die Hernmung dieses Enzyms erfolgt vornehmiich durch metabolische Azidose [38].…”