Carboxyl fuchsine-based colored particles for application to immunodetection

Preparation of dyed polymer microspheres by a physical–chemical dual-binding method and their application in lateral flow immunoassay

“…The traditional preparation methods of dyed microspheres can be divided into two categories according to the combination of microspheres and dyes: (1) physical methods, including the swelling method 21–23 and embedding method; 24–26 (2) chemical methods, including the copolymerization method 27–29 and surface covalent bonding method. 30–32 The swelling method typically penetrates the dye into the polymer chain with the organic solvent, and then the dyes are immobilized after selectively removing the swelling agent.…”