Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing

Abstract

BackgroundNonscar wound healing is a desirable treatment for cutaneous wounds worldwide. Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.PurposeTo overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.MethodsCMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering. Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC. Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods. The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.ResultsThe release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner. CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes. In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone. Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition. CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.ConclusionThe prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.

Nanomaterials for Wound Healing and Infection Control

“…Cathelicidin-carboxymethyl chitosan nanoparticles only enhanced cell migration, without influencing keratinocyte growth. Moreover, in later stages of wound closure, the ratio of collagens I and III favored non-scaring healing [46].…”

The dual anti‐caries effect of carboxymethyl chitosan nanogel loaded with chimeric lysin ClyR and amorphous calcium phosphate

“…In this study, CMC-ClyR-ACP nanogel was successfully synthesized. We speculate that, since CMC is negatively charged (pI~5.14) and ClyR is positively charged (pI~8, as predicted by online software Expasy) at pH 7.4, it is likely for these two compounds to bind to each other mainly by electrostatic interactions coupled with hydrogen bonding and van der Waals forces, as other studies have shown [29,35]. In the CMC-ClyR-ACP nanogel, CMC could still maintain calcium and phosphorus at an amorphous state.…”

<p>Simultaneous Intramuscular And Intranasal Administration Of Chitosan Nanoparticles–Adjuvanted <em>Chlamydia</em> Vaccine Elicits Elevated Protective Responses In The Lung</p>

“…Chitosan nanoparticles were considered as an effective delivery system of the vaccine due to the advantages of biocompatible, biodegradable, mucoadhesive, polycationic, and immunomodulatory properties 36,37. In this study, we explored the effectiveness of CNPs as an immune adjuvant and vaccine delivery system by adsorbing the recombinant Ags on CNPs.…”