Carcinogenic Human Liver Fluke: Current Status of Opisthorchis viverrini Metacercariae in Nakhon Ratchasima, Thailand

Kaewpitoon, Natthawut; Kaewpitoon, Soraya J; Ueng-arporn, Naporn; Rujirakul, Ratana; Churproong, Seekaow; Matrakool, Likit; Auiwatanagul, Suprakrit; Sripa, Banchob

doi:10.7314/apjcp.2012.13.4.1235

Cited by 13 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these, 12.3% were positive for OV MC infection. These infected fish were predominantly C. armatus , C. repasson , P. proctzysron , Hampala macrolepidota , and H. dispar [22]. In contrast, no OV MC-infected fish were found in 3 species (52 Puntius leiacanthus , 22 C. armatus , and 5 H. dispar ) in a study conducted in the year 2000 in the same province [23].…”

Section: Discussionmentioning

confidence: 99%

Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand

et al. 2013

View full text Add to dashboard Cite

To increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of Opisthorchis viverrini metacercariae (OV MC) was investigated in freshwater fish obtained from 20 provinces in northeastern Thailand between April 2011 and February 2012. A cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. Fish, were collected in each of the rainy and winter seasons from each province. Fish were identified, counted, weighed, and digested using pepsin-HCl. Samples were examined for OV MC by a sedimentation method, and metacercariae were identified under a stereomicroscope. OV MC were found in 6 species of fish; i.e., Cyclocheilichthys armatus, Puntius orphoides, Hampala dispar, Henicorhynchus siamensis, Osteochilus hasselti, and Puntioplites proctozysron from localities in 13 provinces. Among the sites where OV MC-infected fish were found, 70.0% were dams, 23.7% were ponds/lakes, and 7.7% were rivers. The mean intensity of OV MC ranged from 0.01 to 6.5 cysts per fish (or 1.3-287.5 cysts per kg of fish). A high mean intensity of OV MC per fish (>3 cysts) was found in 5 provinces: Amnat Charoen (6.5 cysts), Nakhon Phanom (4.3), Mukdahan (4.1), Khon Kaen, (3.5) and Si Sa Ket (3.4). In conclusion, OV MC are prevalent in natural cyprinid fish, with the infection rate varying according to fish species and habitats.

show abstract

Section: Discussionmentioning

confidence: 99%

Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Regarding the status of infection, it was examined by the presence of parasite eggs in feces and the worm in the bile duct. Previous studies reported the O. viverrini uterus was filled with eggs after 20–23 days and eggs were detected in the feces on day 26 ( Nithikathkul et al ., 2007 ; Kaewpitoon et al ., 2012 ). The absence of O. viverrini eggs in mice and their presence in hamsters from D28 to D56 in this study suggests the difference in host susceptibility to O. viverrini infection in these two animal models.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts

Thongrin,

Suyapoh,

Wendo

et al. 2023

Open Vet J

View full text Add to dashboard Cite

Background: Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced. Aim: This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their association with proliferative lesions in the early development of CCA from susceptible and non-susceptible animal models. Methods: Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and non-susceptible animal models, were used in this comparative study. The animals were infected with 50 O. viverrini metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 post-infection (p.i.), five animals were randomly selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the inflammatory cell types. Kruskal-Wallis and Mann-Whitney tests were applied to assess all semi-quantitative and quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value <0.05. Results: The results demonstrated that mice had different patterns of infiltrating cell types when compared to hamsters. This suggested that the cellular response to the infection in mice occurred earlier than that in hamsters. The response in mice reached its peak at D7 to D14 and then rapidly declined at D28. In contrast, although the inflammatory response in hamsters started slowly, the response reached the peak at D28 and maintained a high level until D56. Significant differences in the number of inflammatory cells between mice and hamsters were seen at D1 (p=0.047), D7 (p=0.049), D28 (p=0.040), and D56 (p<0.040). Conclusion: The inflammatory responses to O. viverrini infection in the non-susceptible animal model occurred and declined earlier while the response in the susceptible animal model occurred later in a gradual manner. Both rodents are suitable animal models for the studies of opisthorchiasis susceptibility.

show abstract

“…The study revealed an infection rate of 12.3% (79 out of 640 fish), with Cyclocheilichthys armatus, Cyclocheilichthys repasson, Hampala dispar, Puntioplites proctzysron, and Hampala macrolepitota being the predominant infected species 25 .…”

Section: Life Cycle Of O Viverrinimentioning

confidence: 92%

“…During a thorough surveillance of O. viverrini infection in cyprinid fish, a total of 640 fish from 32 different regions in Nakhon Ratchasima Province in northeastern Thailand were subjected to investigation for O. viverrini metacercariae infection between the years 2010 and 2011 25 .…”

Section: Life Cycle Of O Viverrinimentioning

confidence: 99%

The Past and Present Situation of Opisthorchis viverrini Infection in Thailand

Buathong,

Charoensuk,

Suwannahitatorn

2024

Vajira Med J

View full text Add to dashboard Cite

Opisthorchis viverrini, a major foodborne trematode, remains a major public health concern in Thailand, particularly in the northern and northeastern provinces. O. viverrini can be transferred to people and reservoir hosts through the consumption of uncooked cyprinid fish with metacercariae. Chronic infection with O. viverrini can lead to a severe condition known as cholangiocarcinoma (CCA). According to the Nationwide Hospital Admission Database, Thailand has experienced the highest incidence of CCA globally in recent years. Particularly, between 2009 and 2013, there was an incidence rate of 14.6 cases per 100,000 individuals within the population and a corresponding mortality rate (MR) of 14%. In addition, high incidence rates of CCA were reported in Khon Kaen Province from 1989 to 2018, with a rate of 36.1 per 100,000 person-years in men and 14.4 per 100,000 person-years in women. Despite the reduction in the overall prevalence of O. viverrini at the national level, several studies have indicated that the infection remains highly prevalent in some communities in the northeastern and northern regions. Some communities have demonstrated a prevalence of O. viverrini exceeding 20%, and rates reaching as high as 45.7%. Therefore, in populations with a high prevalence, programs to control this parasite should be implemented, including monitoring eating habits. This review article provides a comprehensive overview of the current distribution of O. viverrini in Thailand, outlining its pathophysiology, background, and preventive and control strategies. In addition, this review suggests that individuals in high-risk communities should act to eradicate liver fluke, as this is crucial for the overall health of the community.

show abstract

Carcinogenic Human Liver Fluke: Current Status of Opisthorchis viverrini Metacercariae in Nakhon Ratchasima, Thailand

Cited by 13 publications

References 18 publications

Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand

Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand

Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts

The Past and Present Situation of Opisthorchis viverrini Infection in Thailand

Contact Info

Product

Resources

About