Cardiac Telocytes and Fibroblasts in Primary Culture: Different Morphologies and Immunophenotypes

Bei, Yihua; Zhou, Qing; Fu, Siyi; Lv, Dongchao; Chen, Ping; Chen, Yuanyuan; Wang, Fei; Xiao, Junjie

doi:10.1371/journal.pone.0115991

Cited by 93 publications

(84 citation statements)

References 63 publications

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“…30. After gerbils were killed, the prostate was dissected under sterile conditions and maintained in Hanks' balanced salt solution (HBSS, R21–022‐CV; Corning, Corning, NY,USA) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin (R30–002‐CI; Corning) and 0.01 mM HEPES(H3375; Sigma Aldrich, St.Louis, MO,USA).…”

Section: Methodsmentioning

confidence: 99%

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Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Sanches

Maldarine

Zani

et al. 2017

J Cellular Molecular Medi

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Telocytes are CD34‐positive interstitial cells, known to exert several functions, one of which is a role in tissue organisation, previously demonstrated by telocytes in the myocardium. The existence of telocytes in the prostate has recently been reported, however, there is a lack of information regarding the function of these cells in prostate tissue, and information regarding the possible role of these cells in prostatic development. This study used immunofluorescence techniques in prostate tissue and prostatic telocytes in culture to determine the relationship between telocytes and prostate morphogenesis. Furthermore, immunofluorescent labelling of telocytes was performed on prostate tissue at different stages of early postnatal development. Initially, CD34‐positive cells are found at the periphery of the developing alveoli, later in the same region, c‐kit‐positive cells and cells positive for both factors are verified and CD34‐positive cells were predominantly observed in the interalveolar stroma and the region surrounding the periductal smooth muscle. Fluorescence assays also demonstrated that telocytes secrete TGF‐β1 and are ER‐Beta (ERβ) positive. The results suggest that telocytes play a changing role during development, initially supporting the differentiation of periductal and perialveolar smooth muscle, and later, producing dense networks that separate alveoli groups and form a barrier between the interalveolar region and periurethral smooth muscle. We conclude that telocytes play a relevant role in prostate tissue organisation during postnatal development.

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Section: Methodsmentioning

confidence: 99%

“…30. After three washes with PBS, telocytes were fixed in 4% paraformaldehyde for 30 min., washed with PBS, then permeabilised with 0.5% TritonX‐100 for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Sanches

Maldarine

Zani

et al. 2017

J Cellular Molecular Medi

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“…Nevertheless, CD34 is not a perfect marker, because endoneurial fi broblasts are also CD34 positive, similarly to TCs (5). Differences in expression of other signifi cant markers between TCs, fi broblasts, pericytes and neurons are shown in Table 1 (18)(19)(20)(21).…”

Section: The Similarity To Other Cellsmentioning

confidence: 99%

“…Thus far, it is confi rmed that cardiac TCs express such molecules as: CD34, c-kit (CD117), PDGFR-α and -β, vimentin, CD29, Nanog, Sca-1 (13,18,19). CD34, the most reliable marker of TCs, is not uniformly expressed by cardiac TCs from conception to death -in embryonic life TCs are negative for CD34 and after birth they gradually gain CD34 expression (14,16).…”

Section: Molecular Characteristics Of Cardiac Tcsmentioning

confidence: 99%

“…Based on the example of heart valves, TCs' network helps in balanced force distribution in tissue and enables to maintain proper valve fl exibility (35). TCs are also supposed to be a source of cardiac mesenchymal cells, because they express mesenchymal marker CD29 and have the ability to lose expression of CD34 (19).…”

Section: Physiological Functions Of Cardiac Tcsmentioning

confidence: 99%

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A comprehensive guide to telocytes and their great potential in cardiovascular system

Kucybała¹,

Janas²,

Ciuk³

et al. 2017

BLL

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Telocytes, a recently discovered type of interstitial cells, have a very distinctive morphology -the small cell body with long extensions, named telopodes. In our review, apart from introducing general aspects of telocytes, we focus on properties, functions and future potential of those cells in cardiovascular system. However, physiological functions of telocytes in cardiovascular system are still regarded as quite enigmatic. Previous studies claim that they play a role in organogenesis and regeneration, bioelectrical signalling, mechanoelectrical coupling, anti-oxidative protection, angiogenesis and regulation of blood fl ow. As well, they are presumably connected with the presence of blood-myocardium barrier and proper organisation of extracellular matrix. Moreover, there exists a signifi cant link between the quantity of telocytes in tissue and numerous cardiovascular diseases such as: myocardial infarction, cardiomyopathies, systemic sclerosis, heart failure, atrial fi brillation, isolated atrial amyloidosis, myxomatous valve degeneration and hyperplastic consequences of vascular injury. Thanks to their unique properties, telocytes might be a breakthrough in treatment of cardiovascular diseases, as they may be effective in reversing effects of myocardial infarction. Telocytes also may play a major role in tissue engineering -they might be the key factor in creating stable and effi cient vascular network in larger synthetic tissues or organs (Tab. 1, Fig. 3, Ref. 53). Text in PDF www.elis.sk.

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Explant culture: A relevant tool for the study of telocytes

Sanches

Maldarine

Tamarindo

et al. 2020

Cell Biology International

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Telocytes are cells present in the stroma of various tissues including the prostate. The detection of telocytes is still very much dependent on obtaining ultrastructural data that show the presence of telopodes, which are cytoplasmic projections that alternate between dilated regions, the podoms, and thin segments, the podomers. These structures are the distinctive characteristics of the telocytes. Thus, in vitro assays are important for the study of telocytes, which are more easily identified in culture, which also enables the experimental manipulation of these cells. The isolation of telocytes per se does not allow the analysis of the behavior of these cells in relation to other cell types in a given organ. In this sense, in the prostate, explants could be a useful tool for the study of telocytes. The present study obtained prostatic explants and evaluated the influence of recombinant proteins, scattering factor (SCF) and stromal‐derived factor 1 (SDF‐1), which could impact on the migration of CD34‐positive cells. Telocytes migrate out of explants and SDF‐1 stimulates the proliferation and formation of telocyte networks in vitro. Telocytes are not smooth muscle cell progenitors in the prostate; on the contrary, they are CD90‐ and CD44‐negative cells and, hence, have limited progenitor capacity. The present study demonstrated that explants are useful tools to elucidate the nature of telocytes and their functions.

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Cardiac Telocytes and Fibroblasts in Primary Culture: Different Morphologies and Immunophenotypes

Cited by 93 publications

References 63 publications

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

A comprehensive guide to telocytes and their great potential in cardiovascular system

Explant culture: A relevant tool for the study of telocytes

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