Cardiac Tissue Creation with the Kenzan Method

Matsushita, Hiroshi; Nguyen, Vivian; Nurminsky, Katherine; Hibino, Narutoshi

doi:10.1007/978-3-030-58688-1_8

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Gaps and Role Of Bioprinting To Address Issues1

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2021

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“…This array of needles bioprint cell-laden spheroids into predesigned contiguous temporary support with micron-level precision [137]. Then, the spheroid-based structure is cultured until the spheroids fuse into cellular aggregates and synthesize their own ECM [138]; spheroids generated using this method have uniform shapes and sizes. During insertion, smaller spheroids have the potential to disintegrate on the needle [137].…”

Section: Gaps and Role Of Bioprinting To Address Issuesmentioning

confidence: 99%

Prevascularized Micro-/Nano-Sized Spheroid/Bead Aggregates for Vascular Tissue Engineering

et al. 2021

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Efficient strategies to promote microvascularization in vascular tissue engineering, a central priority in regenerative medicine, are still scarce; nano- and micro-sized aggregates and spheres or beads harboring primitive microvascular beds are promising methods in vascular tissue engineering. Capillaries are the smallest type and in numerous blood vessels, which are distributed densely in cardiovascular system. To mimic this microvascular network, specific cell components and proangiogenic factors are required. Herein, advanced biofabrication methods in microvascular engineering, including extrusion-based and droplet-based bioprinting, Kenzan, and biogripper approaches, are deliberated with emphasis on the newest works in prevascular nano- and micro-sized aggregates and microspheres/microbeads.

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Section: Gaps and Role Of Bioprinting To Address Issuesmentioning

confidence: 99%