Cardiomyocyte Differentiation from Human Embryonic Stem Cells

Mazzotta, Silvia; Lynch, Adam; Hoppler, Stefan

doi:10.1007/978-1-4939-8597-5_5

Cited by 5 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Primary cardiomyocytes isolated from rodent hearts have characteristic differences like a different electrophysiology in comparison to the human ones. Therefore, cardiomyocytes derived from human ESCs or iPSCs are the predominant human cell resource [328,329].…”

Section: Differentiation Of Human Induced Pluripotent Stem Cells Imentioning

confidence: 99%

Human Induced Pluripotent Stem-Cell-Derived Cardiomyocytes as Models for Genetic Cardiomyopathies

Brodehl

Ebbinghaus

Deutsch

et al. 2019

IJMS

View full text Add to dashboard Cite

In the last few decades, many pathogenic or likely pathogenic genetic mutations in over hundred different genes have been described for non-ischemic, genetic cardiomyopathies. However, the functional knowledge about most of these mutations is still limited because the generation of adequate animal models is time-consuming and challenging. Therefore, human induced pluripotent stem cells (iPSCs) carrying specific cardiomyopathy-associated mutations are a promising alternative. Since the original discovery that pluripotency can be artificially induced by the expression of different transcription factors, various patient-specific-induced pluripotent stem cell lines have been generated to model non-ischemic, genetic cardiomyopathies in vitro. In this review, we describe the genetic landscape of non-ischemic, genetic cardiomyopathies and give an overview about different human iPSC lines, which have been developed for the disease modeling of inherited cardiomyopathies. We summarize different methods and protocols for the general differentiation of human iPSCs into cardiomyocytes. In addition, we describe methods and technologies to investigate functionally human iPSC-derived cardiomyocytes. Furthermore, we summarize novel genome editing approaches for the genetic manipulation of human iPSCs. This review provides an overview about the genetic landscape of inherited cardiomyopathies with a focus on iPSC technology, which might be of interest for clinicians and basic scientists interested in genetic cardiomyopathies.

show abstract

Section: Differentiation Of Human Induced Pluripotent Stem Cells Imentioning

confidence: 99%