Cardiomyocyte Functions Couple with Left Ventricular Geometric Patterns in Hypertension.

Abstract

Previous studies have suggested the prognostic significance of left ventricular (LV) geometric patterns in essential hypertension.However, the relation between cardiomyocyte functional changes and LV geometric patterns has not been clarified. This study was designed to assess the morphological and functional changes in isolated myocytes derived from different LV geometric patterns in hypertension. After 2-3 weeks of a high-salt (8%) diet from the age of 6 weeks, 20 Dahl salt-sensitive (DS) rats were classified into the following three groups on the basis of an echocardiographically determined LV mass index and the relative wall thickness:concentric hypertrophy (11), eccentric hypertrophy (4), and concentric remodeling

Differential Induction of Protein Kinase C Isoforms at the Cardiac Hypertrophy Stage and Congestive Heart Failure Stage in Dahl Salt-Sensitive Rats

“…To date, there have been few reports comparing the patterns of all kinds of PKC expression including aPKCs between cardiac hypertrophy and heart failure in the same model system. Several reports have suggested that Dahl salt-sensitive rats are a suitable model for the comparison between cardiac hypertrophy and congestive heart failure in this loading protocol (18)(19)(20)(21)(22). In this study, we found different PKC isoform-specific patterns of upregulation at the cardiac hypertrophy stage and heart failure stage.…”

“…Systolic blood pressure was measured by the tail-cuff method of Friedman and Freed at 6, 8 and 12 weeks of age (23). Rats were killed at 11 weeks of age at the cardiac hypertrophy stage or at 16 weeks at the congestive heart failure stage (18)(19)(20)(21)(22). The hearts were dissected out and immediately frozen in liquid nitrogen for Western blot analysis.…”

Impaired Ca2+ Handling in Perfused Hypertrophic Hearts from Dahl Salt-Sensitive Rats

“…These studies demonstrated the importance of SR in establishing the force-frequency relationship. An impaired force-frequency relationship was also observed in cardiomyocytes from hypertrophic hearts of DS rats (17). The force-frequency relationship is more negative in both human and animal diseased hearts (11,35).…”

“…This strain is a genetic model of hypertension in which the progression from hypertrophy to failure can be observed without surgical intervention (16,17). Fura-2, a fluorescent indicator, was used to measure intracellular Ca 2 in the perfused heart.…”

Enhancement of Glomerular Platelet-Derived Growth Factor β-Receptor Tyrosine Phosphorylation in Hypertensive Rats and Its Inhibition by Calcium Channel Blocker

“…SHRSP and DS-rats are two commonly used rat hypertensive models that exhibit severe glomerular injury (12)(13)(14)(15). Spontaneously hypertensive rats (SHR), on the other hand, show no significant glomerular injury despite their hypertension.…”