Cardiomyopathies

Marı́n-Garcı́a, José

doi:10.1016/b978-0-12-404599-6.00012-3

Cited by 3 publications

(2 citation statements)

References 439 publications

(332 reference statements)

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“…[ 35 , 36 ] Myosin light chains belong to a superfamily of calcium‐binding proteins that are characterized by highly conserved helix‐loop‐helix calcium‐binding domains (named EF hands). [ 35 , 36 ] Since it is known that the IgE binding capacity of other EF hand calcium binding allergens depends on the presence of calcium, [ 37 ] we analyzed in an ELISA the effect of calcium on the IgE binding capacity of rMYL1 and saw that neither the presence nor the absence of calcium had an effect on the molecule's IgE binding capacity (data not shown). Our results indicate that the IgE reactivity of EF hand calcium binding allergens is not always calcium dependent.…”

Section: Discussionmentioning

confidence: 99%

Bos d 13, A Novel Heat‐Stable Beef Allergen

Román‐Carrasco,

Klug,

Hemmer

et al. 2023

Molecular Nutrition Food Res

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ScopeRed meat, a staple food of Western diets, can also induce IgE‐mediated allergic reactions. Yet, apart from the heat‐labile protein serum albumin and the carbohydrate α‐Gal, the molecules causing allergic reactions to red meat remain unknown.Methods and resultsIgE reactivity profiles of beef‐sensitized individuals are analyzed by IgE‐immunoblotting with protein extracts from raw and cooked beef. Two IgE‐reactive proteins are identified by peptide mass fingerprinting as myosinlight chain 1 (MYL1) and myosin light chain 3 (MYL3) in cooked beef extract and are designated Bos d 13 isoallergens. MYL1 and MYL3 are produced recombinantly in Escherichia coli. ELISAs proved their IgE reactivity and circular dichroism analysis showed that they represent folded molecules with remarkable thermal stability. In vitro gastrointestinal digestion experiments showed the higher stability of rMYL1 as compared to rMYL3. Exposure of a monolayer of Caco–2 cells to rMYL1 indicated that the molecule is able to cross intestinal epithelial cells without disturbing the integrity of the tight junctions, suggesting the sensitizing capacity of MYL1.ConclusionMYLs are identified as novel heat‐stable bovine meat allergens.

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Section: Discussionmentioning

confidence: 99%

Bos d 13, A Novel Heat‐Stable Beef Allergen

Román‐Carrasco,

Klug,

Hemmer

et al. 2023

Molecular Nutrition Food Res

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“…The SGCD gene encodes the sarcolemmal transmembrane glycoprotein delta -sarcoglycan, one of four sarcoglycan proteins ( alpha-, beta-, gamma- , and delta -SG) that are highly expressed in cardiac and skeletal muscle [ 1 ]. These four glycoproteins form the sarcoglycan complex, which in turn is part of the dystrophin–glycoprotein complex that stabilizes the sarcolemma.…”

Section: Introductionmentioning

confidence: 99%

SGCD Missense Variant in a Lagotto Romagnolo Dog with Autosomal Recessively Inherited Limb-Girdle Muscular Dystrophy

Brunetti,

Bacci,

Abbate

et al. 2023

Genes

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An 8-month-old female Lagotto Romagnolo dog was presented for a 1-month history of an initial severe reluctance to move, rapidly progressing to a marked stiff gait and progressive muscular weakness and evolving to tetraparesis, which persuaded the owner to request euthanasia. A primary muscle pathology was supported by necropsy and histopathological findings. Macroscopically, the muscles were moderately atrophic, except for the diaphragm and the neck muscles, which were markedly thickened. Histologically, all the skeletal muscles examined showed atrophy, hypertrophy, necrosis with calcification of the fibers, and mild fibrosis and inflammation. On immunohistochemistry, all three dystrophin domains and sarcoglycan proteins were absent. On Western blot analysis, no band was present for delta sarcoglycan. We sequenced the genome of the affected dog and compared the data to more than 900 control genomes of different dog breeds. Genetic analysis revealed a homozygous private protein-changing variant in the SGCD gene encoding delta- sarcoglycan in the affected dog. The variant was predicted to induce a SGCD:p.(Leu242Pro) change in the protein. In silico tools predicted the change to be deleterious. Other 770 Lagotto Romagnolo dogs were genotyped for the variant and all found to be homozygous wild type. Based on current knowledge of gene function in other mammalian species, including humans, hamsters, and dogs, we propose the SGCD missense variant as the causative variant of the observed form of muscular dystrophy in the index case. The absence of the variant allele in the Lagotto Romagnolo breeding population indicates a rare allele that has appeared recently.

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Cardiovascular Biomarkers: Lessons of the Past and Prospects for the Future

Omran

Kyrou

Osman

et al. 2022

IJMS

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Cardiovascular diseases (CVDs) are a major healthcare burden on the population worldwide. Early detection of this disease is important in prevention and treatment to minimise morbidity and mortality. Biomarkers are a critical tool to either diagnose, screen, or provide prognostic information for pathological conditions. This review discusses the historical cardiac biomarkers used to detect these conditions, discussing their application and their limitations. Identification of new biomarkers have since replaced these and are now in use in routine clinical practice, but still do not detect all disease. Future cardiac biomarkers are showing promise in early studies, but further studies are required to show their value in improving detection of CVD above the current biomarkers. Additionally, the analytical platforms that would allow them to be adopted in healthcare are yet to be established. There is also the need to identify whether these biomarkers can be used for diagnostic, prognostic, or screening purposes, which will impact their implementation in routine clinical practice.

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Cardiomyopathies

Cited by 3 publications

References 439 publications

Bos d 13, A Novel Heat‐Stable Beef Allergen

Bos d 13, A Novel Heat‐Stable Beef Allergen

SGCD Missense Variant in a Lagotto Romagnolo Dog with Autosomal Recessively Inherited Limb-Girdle Muscular Dystrophy

Cardiovascular Biomarkers: Lessons of the Past and Prospects for the Future

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