Cardiorespiratory ontogeny and response to environmental hypoxia of larval spiny lobster, Sagmariasus verreauxi

Fitzgibbon, Quinn P.; Ruff, Nicole; Battaglene, SC

doi:10.1016/j.cbpa.2015.02.007

Cited by 7 publications

(5 citation statements)

References 58 publications

(87 reference statements)

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“…Future experiments should account for this initial variability. Moreover, future studies should determine whether later larval life-stages are more sensitive due to their larger mass and diffusion distances or because of their rudimentary cardiorespiratory anatomy, as suggested for other crustacean species (Fitzgibbon et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…They have complex life cycles, with contrasting physiological characteristics (e.g. related to oxygen uptake through either diffusion or convection) that differ not only across species, but also within species across different stages of development (Walther et al 2009 ; Anger 2001 ; Storch et al 2011 ; Jensen et al 2013 ; Alter et al 2015 ; Fitzgibbon et al 2015 ). For eggs and probably larvae, oxygen required for metabolism is obtained primarily via diffusion.…”

Section: Introductionmentioning

confidence: 99%

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Differences in the respiratory response to temperature and hypoxia across four life-stages of the intertidal porcelain crab Petrolisthes laevigatus

et al. 2018

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For aquatic breathers, hypoxia and warming can act synergistically causing a mismatch between oxygen supply (reduced by hypoxia) and oxygen demand (increased by warming). The vulnerability of these species to such interactive effects may differ during ontogeny due to differing gas exchange systems. This study examines respiratory responses to temperature and hypoxia across four life-stages of the intertidal porcelain crab Petrolisthes laevigatus. Eggs, megalopae, juveniles and adults were exposed to combinations of temperatures from 6 to 18 °C and oxygen tensions from 2 to 21 kPa. Metabolic rates differed strongly across life-stages which could be partly attributed to differences in body mass. However, eggs exhibited significantly lower metabolic rates than predicted for their body mass. For the other three stages, metabolic rates scaled with a mass exponent of 0.89. Mass scaling exponents were similar across all temperatures, but were significantly influenced by oxygen tension (the highest at 9 and 14 kPa, and the lowest at 2 kPa). Respiratory responses across gradients of oxygen tension were used to calculate the response to hypoxia, whereby eggs, megalopae and juveniles responded as oxyconformers and adults as oxyregulators. The thermal sensitivity of the metabolic rates (Q10) were dependent on the oxygen tension in megalopae, and also on the interaction between oxygen tension and temperature intervals in adults. Our results thus provide evidence on how the oxygen tension can modulate the mass dependence of metabolic rates and demonstrate changes in respiratory control from eggs to adults. In light of our results indicating that adults show a good capacity for maintaining metabolism independent of oxygen tension, our study highlights the importance of assessing responses to multiple stressors across different life-stages to determine how vulnerability to warming and hypoxia changes during development.Electronic supplementary materialThe online version of this article (10.1007/s00227-018-3406-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differences in the respiratory response to temperature and hypoxia across four life-stages of the intertidal porcelain crab Petrolisthes laevigatus

et al. 2018

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“…Thoa et al [ 17 ]. A number of studies have shown that lobster larval survival depends strongly on various rearing conditions and management practices, such as water quality parameters [ 3 , 34 ], light regimes [ 23 ], background of rearing facility and stocking density [ 35 ], supplementary feeds and diets [ 36 , 37 ]. In the present study, attempts were made to provide a homogeneous rearing environments and husbandry practices, but variation in the rearing tanks and hatchery practices between years would have affected survivals.…”

Section: Discussionmentioning

confidence: 99%

Can metamorphosis survival during larval development in spiny lobster Sagmariasus verreauxi be improved through quantitative genetic inheritance?

et al. 2018

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BackgroundOne of the major impediments to spiny lobster aquaculture is the high cost of hatchery production due to the long and complex larval cycle and poor survival during the many moult stages, especially at metamorphosis. We examined if the key trait of larval survival can be improved through selection by determining if genetic variance exists for this trait. Specifically, we report, for the first time, genetic parameters (heritability and correlations) for early survival rates recorded at five larval phases; early-phyllosoma stages (instars 1–6; S1), mid-phyllosoma stages (instars; 7–12; S2), late-phyllosoma stages (instars 13–17; S3), metamorphosis (S4) and puerulus stage (S5) in hatchery-reared spiny lobster Sagmariasus verreauxi.ResultsThe data were collected from a total of 235,060 larvae produced from 18 sires and 30 dams over nine years (2006 to 2014). Parentage of the offspring and full-sib families was verified using ten microsatellite markers. Analysis of variance components showed that the estimates of heritability for all the five phases of larval survival obtained from linear mixed model were generally similar to those obtained from threshold logistic generalised models (0.03–0.47 vs. 0.01–0.50). The heritability estimates for survival traits recorded in the early larval phases (S1 and S2) were higher than those estimated in later phases (S3, S4 and S5). The existence of the additive genetic component in larval survival traits indicate that they could be improved through selection. Both phenotypic and genetic correlations among the five survival measures studied were moderate to high and positive. The genetic associations between successive rearing periods were stronger than those that are further apart.ConclusionsOur estimates of heritability and genetic correlations reported here in a spiny lobster species indicate that improvement in the early survival especially during metamorphosis can be achieved through genetic selection in this highly economic value species.Electronic supplementary materialThe online version of this article (10.1186/s12863-018-0621-z) contains supplementary material, which is available to authorized users.

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“…In barnacles, a specialized epithelial layer with a potential dual role in osmoregulation and biomineralization was identified 15 , portraying a clear pattern of specialized epithelia that enable respiration and ion exchange across mineralized cuticles in distantly related crustacean taxa. It was suggested that increased temperatures and decreased dissolved oxygen in marine environments might significantly affect the vulnerable spiny lobster larvae due to their immature respiratory system 16 . Better understanding of the molecular mechanisms which facilitate the gas/ion exchange capacity across mineralized cuticles might prove useful in developing bio-inspired materials and will also contribute to our understanding of the potential consequences climate change holds for species with confounding respiratory capacity.…”

Section: Introductionmentioning

confidence: 99%

Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

Ventura

Nguyen

Fitzgibbon

et al. 2019

Sci Rep

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Literature on the cuticle formation in larval stages of the diverse group of decapod crustaceans is lacking, as opposed to a wealth of knowledge in several insect groups. Here we provide the first glimpse of the cuticular organisation in larvae of the eastern spiny lobster Sagmariasus verreauxi . A bioinformatic approach applied to S . verreauxi transcriptome through metamorphosis identified for the first time a small secreted protein with multiple isoforms that is highly expressed in crustacean larvae. This protein, named crustacean larval factor (Clf) shares structural characteristics with insect follicle cell protein 3 (FCP3), an insect-specific, rapidly evolving protein, with spatial-temporal regulated expression that is restricted to follicular cells during the production of the vitellin coat. Furthermore, we identified the FCP3 domain in additional structural proteins in multiple arthropod groups. Recombinant Clf inhibited in vitro calcium carbonate crystalline precipitation, in keeping with the finding that the spiny lobster larval cuticle is mainly composed of amorphous calcium carbonate. In addition, the recombinant Clf was shown to bind chitosan. Taken together, this research identifies two novel structural domains with lineage-specific expansion across arthropods. In crustaceans, Clf is found predominantly in larvae and the spatial-temporal regulated FCP3 factor occurs as a domain identified in multiple structural proteins across arthropods. Given the shared ten cysteines backbone between the Clf and FCP domains, a shared evolution is suggested and should be further explored.

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Cardiorespiratory ontogeny and response to environmental hypoxia of larval spiny lobster, Sagmariasus verreauxi

Cited by 7 publications

References 58 publications

Differences in the respiratory response to temperature and hypoxia across four life-stages of the intertidal porcelain crab Petrolisthes laevigatus

Differences in the respiratory response to temperature and hypoxia across four life-stages of the intertidal porcelain crab Petrolisthes laevigatus

Can metamorphosis survival during larval development in spiny lobster Sagmariasus verreauxi be improved through quantitative genetic inheritance?

Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

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