2010
DOI: 10.1007/s10295-010-0762-1
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Carob pulp as raw material for production of the biocontrol agent P. agglomerans PBC-1

Abstract: Large-scale production has been the major obstacle to the success of many biopesticides. The spreading of microbial biocontrol agents against postharvest disease, as a safe and environmentally friendly alternative to synthetic fungicides, is quite dependent on their industrial mass production from low-cost raw materials. Considerable interest has been shown in using agricultural waste products and by-products from food industry as nitrogen and carbon sources. In this work, carob pulp aqueous extracts were used… Show more

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Cited by 29 publications
(20 citation statements)
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“…High yield was obtained using 5 g/l of sucrose as carbon source. A much lower cost medium with high biomass productivity of this biocontrol agent was developed using byproducts from the carob industry (Manso et al 2010b), and from the citrus industry (Manso et al, unpublished data). Costa et al (2001) using another strain of P. agglomerans (CPA-2) optimized its production by using dry beer yeast as nitrogen source.…”
Section: Production Of a Biological Control Agentmentioning
confidence: 99%
“…High yield was obtained using 5 g/l of sucrose as carbon source. A much lower cost medium with high biomass productivity of this biocontrol agent was developed using byproducts from the carob industry (Manso et al 2010b), and from the citrus industry (Manso et al, unpublished data). Costa et al (2001) using another strain of P. agglomerans (CPA-2) optimized its production by using dry beer yeast as nitrogen source.…”
Section: Production Of a Biological Control Agentmentioning
confidence: 99%
“…Broccoli byproducts may be used as a source of bioactive ingredients to design novel beverages-using organic green tea as a food matrix-presenting improved physical quality, phytochemical composition and antioxidant capacity (Dominguez-Perles et al, 2011). Carob pulp aqueous extracts were used as carbon source in the production of the biocontrol agent Pantoea agglomerans PBC-1, usable as bio-pesticide (Manso et al, 2010). Tests proved that the aqueous extracts exhibit a high potential of this by-product as a carbon source to produce high amounts of biomass of the biocontrol agent at low cost.…”
Section: Other Vegetablesmentioning
confidence: 99%
“…Up to four leaching tests were conducted, with half initial ratio, to take full advantage of all soluble sugars in the carob pulp. These mixtures were homogenized at 150 rpm, 25°C for 1 h as suggested by Manso et al [10,11]. After this period, to clarify the carob pulp extract, the mixtures were centrifuged at 7,500g, at 4°C for 15 min (Beckman J2-MC Centrifuge with a JA14 rotor), filtered through a 0.22-lm membrane filter, and stored at -20°C.…”
Section: Aqueous Carob Pulp Extractionmentioning
confidence: 99%
“…Its price has fluctuated and is about 200 €/ton. The carob seeds represent 10% of the weight of the fruit and the pulp represents the other 90% [10]. Several authors have reported that carob pulp composition has a high content of sugars, mainly sucrose (more than 30%), fructose, and glucose with values of 20-50% [15], 45% [2], 48-56% [22], and 28-82% [4].…”
Section: Introductionmentioning
confidence: 99%
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