Carotenogénesis de cinco cepas del alga &lt;i&gt;Dunaliella&lt;/i&gt; sp. (Chlorophyceae) aisladas de lagunas hipersalinas de Venezuela

Guevara, Miguel Ángel; Lodeiros, César; Gómez, Olga; Lemus, Nathalie; Núñez, Paulino; Romero, L.; Vázquez, Aléikar; Rosales, Nestor

doi:10.15517/rbt.v53i3-4.14593

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…Afify et al (2010) found a similar value, 9.2% dw of lipid for the filamentous cyanobacterium Spirulina platensis. There are many reports in the literature of eukaryotic microalgae that responded to nitrogen starvation with expressive increases in lipid production (Lombardi & Wangersky, 1995;Guevara et al, 2005;Chen et al, 2011;Karatay & Dönmez, 2011), contrasting with our study.…”

Section: Gross Chemical Compositioncontrasting

confidence: 99%

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Setta¹,

Barbarino²,

Passos³

et al. 2014

lajar

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ABSTRACT. Nowadays algal biofuels are considered one of the most promising solutions of global energy crisis and climate change for the years to come. By manipulation of the culture conditions, many algal species can be induced to accumulate high concentrations of particular biomolecules and can be directed to the desired output for each fuel. In this context, the present study involved the assessment of the effects of CO 2 availability and nitrogen starvation on growth and chemical composition of the cyanobacterium Synechococcus subsalsus, testing a fast-growing native strain. The control experiments were performed with Conway culture medium in 12-day batch cultures, in 6-liter flasks and 12 h photoperiod, with addition of 2 L min -1 filtered air to each flask. Other two experimental conditions were also tested: (i) the placement into the cultures of additional dissolved nutrients except nitrogen, one week after the start of growth (N-), and (ii) the input of pure CO 2 into the flasks from the 5 th day of growth (C+). In all cultures, daily cell counts were done throughout the cultivation, as well as measurements of pH and cell biovolumes. Maximum cell yield were found in N-experiments, while cell yields of C+ and control were similar. Dissolved nitrogen was exhausted before the end of the experiments, but dissolved phosphorus was not totally consumed. Protein and chlorophyll-a concentrations decreased from the exponential to the stationary growth phase of all experiments, except for protein in the control. In all experiments, carbohydrate, lipid and total carotenoid increased from the exponential to the stationary growth phase, as an effect of nitrogen limitation. Increments in carbohydrate concentrations were remarkable, achieving more than 42% of the dry weight (dw), but concentrations of lipid were always lower than 13% dw. The addition of pure CO 2 did not cause a significant increase in biomass of S. subsalsus nor generated more lipid and carbohydrate than the other treatments. Nitrogen starvation caused an intense accumulation of carbohydrate, but the increments of lipid were small. Despite the fast growth, the cyanobacterium S. subsalsus has a virtually null potential for biodiesel production, given its low lipid concentrations. The high concentrations of carbohydrate combined with fast growth point to the potential use of this species as raw material for other possible biotechnological processes, after a demonstration of technical and economic viability. Keywords: biodiesel, biomass, cultivation, lipid, microalgae, cyanobacteria, productivity.Evaluación de la utilidad de la cianobacteria marina Synechococcus subsalsus como fuente de biomasa para la producción de biocombustibles RESUMEN. Actualmente los biocombustibles elaborados con algas se consideran una de las soluciones más prometedoras de la crisis energética mundial y el cambio climático para los años venideros. Mediante la manipulación de las condiciones de cultivo, muchas especies de algas pueden ser inducidas para acumular altas concentracione...

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Section: Gross Chemical Compositioncontrasting

confidence: 99%

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Setta¹,

Barbarino²,

Passos³

et al. 2014

lajar

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“…Actually, the increase in lipid content may be species and strain specific (El-Baky et al [ 2004 ]; Pal et al [ 2011 ]; Olofsson et al [ 2014 ]). Dunaliella , Chlorella and Nannochloropsis species are known to respond to nitrogen starvation by increasing lipid production (Lombardi and Wangersky [ 1995 ]; Guevara et al [ 2005 ]; Converti et al [ 2009 ]; Dong et al [ 2013 ]). Also, it is evident that nitrogen is a limiting factor for the growth of many living species (Hansen et al [ 2000 ]; Li et al [ 2010 ]; Park et al [ 2012 ]).…”

Section: Introductionmentioning

confidence: 99%

Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina

Fakhry

Maghraby

2015

Bot Stud

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BackgroundThis batch study deals with the relation between lipid as well as triglyceride contents in Nannochloropsis salina and variation in culture conditions such as nitrogen concentration and temperature.ResultsThe tested parameters caused reduction in growth expressed as cell count, optical density and dry weight, as well strongly involved in lipids and triglycerides accumulation and significantly affected the lipid productivity. At the beginning of the work, the concentration of nitrogen in the medium was reduced to three quarter, half and quarter of the original f2 medium while the temperature kept constant. After that, the optimal nitrogen concentration (quarter of the original media) giving high lipid yield was tested with different temperature degrees from 15 to 35°C with five degree intervals. Although the growth was insignificantly influenced, a considerable increase in lipid and triglyceride (56.1 and 15.1% of dry weight respectively) was observed when the concentration of nitrogen in the medium was reduced to the quarter. Moreover, 59.3% lipid and 17.1% triglyceride on the basis of dry weight were obtained by the combination of 25% nitrogen concentration and 30°C. Simple regressions recommended that the interaction effect of nitrogen limitation and temperature on lipid and triglyceride accumulation was not as fundamental as for nitrogen limitation stress.ConclusionThe degree of nitrogen availability in the combination of temperature effect has been identified as the critical determinant for the maximal production of lipid in N. salina. Nevertheless, major advances in this field can be considered by studying more stresses techniques and genetic strategies.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0085-7) contains supplementary material, which is available to authorized users.

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“…In this context, Cifuentes et al [45] and Guevara et al [46] have reported that the environmental conditions may be partly responsible for the development carotenogenesis in a particular strain of Dunaliella spp.…”

Section: Discussionmentioning

confidence: 99%

Growth and Carotenoid Production in Dunaliella spp. Isolated from Hypersaline Habitats in the Region of Essaouira (Morocco): Effect of NaNO3 Concentration

Charioui¹,

Chikhaoui²,

Banaoui³

et al. 2016

JPP

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Abstract:In order to determine the impact of nitrogen deficiency in medium, growth rate and carotenoids contents were followed during 15 days in two strain Dunaliella spp. (DUN2 and DUN3), isolated respectively from Azla and Idao Iaaza saltworks in the Essaouira region (Morocco). These microalgae were incubated at 25 ± 1 °C with a salinity of 35‰ and continuous light in four growth media with different concentrations of sodium nitrate (NaNO 3 ): 18.75 g/L, 2.5 g/L, 37.5 g/L and 75 g/L. Maximum of cell density was observed under high sodium nitrate concentration during logarithmic phase of growth. The highest specific growth rate was 0.450 × 10 6 ± 0.006 cells/mL and 2.680 × 10 6 ± 0.216 cells/mL, respectively for DUN2 and DUN3. Carotenoids production mean were not stimulated under nitrogen deficiency, and the highest content was showed in DUN2 at high nitrogen concentration (3.210 ± 0.261 μg·mL -1 ) compared with DUN3 strain.

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Carotenogénesis de cinco cepas del alga <i>Dunaliella</i> sp. (Chlorophyceae) aisladas de lagunas hipersalinas de Venezuela

Cited by 14 publications

References 12 publications

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina

Growth and Carotenoid Production in Dunaliella spp. Isolated from Hypersaline Habitats in the Region of Essaouira (Morocco): Effect of NaNO3 Concentration

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