Carotenoid analysis and functional characterization of lycopene cyclases in Zinnia elegans L.

Qing, Hongsheng; Qian, Jieyu; Chen, Jiahong; Jiang, Lingli; Fu, Jianxin; Huang, Xing-Qi; Zhang, Chao

doi:10.1016/j.indcrop.2022.115724

Cited by 4 publications

(4 citation statements)

References 51 publications

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“…The ECOB could produce β-carotene, but γ-carotene was not detected (Figure 5B), suggesting that CsLCYB possesses a strong double β-ring activity (Figure 6). The abovementioned findings are consistent with the functions of LCYBs from most plants and algae, such as A. thaliana [11], Z. elegans [34], H. pluvialis [8], N. oceanica [35], Porph. umbilicalis, and P. yezoensis [13].…”

Section: Discussionsupporting

confidence: 84%

“…In contrast, the co-introduction of LCYB and LCYE from Porph. umbilicalis, P. yezoensis [13], B. fuscopurpurea [14], and Z. elegans L. [34] into lycopene-producing E. coli resulted in a distinct product spectrum in which the level of β-carotene was significantly higher than the level of α-carotene. The production of a high level of α-carotene when co-introducing CsLCYB and CsLCYE might be due to biosynthesis via both δ-carotene and γ-carotene (Figure 6), which is not commonly found in other organisms.…”

Section: Discussionmentioning

confidence: 99%

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Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60

Fang

Liu

et al. 2023

Marine Drugs

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Lutein is a high-value carotenoid with many human health benefits. Lycopene β- and ε-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of lycopene into distinct downstream branches, one of which is the lutein biosynthesis pathway, via α-carotene. Hence, LCYB and LCYE are key enzymes in lutein biosynthesis. In this study, the coding genes of two lycopene cyclases (CsLCYB and CsLCYE) of a lutein-enriched marine green microalga, Chlorella sorokiniana FZU60, were isolated and identified. A sequence analysis and computational modeling of CsLCYB and CsLCYE were performed using bioinformatics to identify the key structural domains. Further, a phylogenetic analysis revealed that CsLCYB and CsLCYE were homogeneous to the proteins of other green microalgae. Subcellular localization tests in Nicotiana benthamiana showed that CsLCYB and CsLCYE localized in chloroplasts. A pigment complementation assay in Escherichia coli revealed that CsLCYB could efficiently β-cyclize both ends of lycopene to produce β-carotene. On the other hand, CsLCYE possessed a strong ε-monocyclase activity for the production of δ-carotene and a weak ε-bicyclic activity for the production of ε-carotene. In addition, CsLCYE was able to catalyze lycopene into β-monocyclic γ-carotene and ultimately produced α-carotene with a β-ring and an ε-ring via γ-carotene or δ-carotene. Moreover, the co-expression of CsLCYB and CsLCYE in E. coli revealed that α-carotene was a major product, which might lead to the production of a high level of lutein in C. sorokiniana FZU60. The findings provide a theoretical foundation for performing metabolic engineering to improve lutein biosynthesis and accumulation in C. sorokiniana FZU60.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60

Fang

Liu

et al. 2023

Marine Drugs

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“…Acnutea ageratum, Agastache foeniculum, Agastache rugosa [425], Bidens ferulaefolia, Calendula officinalis, 'Alice Orange' and 'Alice Yellow' [5], Chrysanthemum carinatum, Chrysanthemum segetum, Coreopsis grandiflora, Coreopsis tinctoria, Coreopsis verticillate, Cosmidium brunette, Begonia argentea, twenty-three Dendranthema grandiflorum [110], Dimorphotheca aurantiaca, Helianthus decapitatis var. Loddon Gold, Helichrysum bracteatum, Hypericum perforatum [5], Hypochoeris radicata, Lantana camara [5,366], Lavandula angustifolia [366], Layia elegans, Layia platyglossa, Momordica charantia, Osmanthus Chenghong Dangui, Osmanthus fragrans [5], Osmanthus fragrans 'Yanhong Gui' [431], Passiflora × belotti, Renealmia Alpinia [366], Rhododendron mole [432], Rudbeckia speciosum, Santolinas teretifolia, Santolinas viridis, Spathiphyllum montanum [366], Senecio scandens, Solidago sp., Tagetes erecta yellow and orange [430], Tagete patula [130], Taraxacum kok-saghyz, Tragopogon pratensis, Tropaeolum majus [5], Ursinia calenduliflora, Venidium decurrens, Zinnia elegans [433], Zinnia elegans 'Dreamland Red', and 'Dreamland Yellow' [433]. [366], Solanum lycopersicum 'M82' [5], Sophora japónica, Spathiphyllum montanum [366], Solanum lycopersicum, Solanum rantonnetii [366], Tabebuia chrysantha, Tagetes erecta [5], Lady Tagetes erecta, Tagetes patula [430], Tagetes patula 'Safari Tangerine' [5], Tecoma capensis, Trachelospermum jasminoides, Theretia peruviana, Thumbergia alata [5], Trachelospermum jasminoides, Trifolium cernnum, Trifolium hybridum, Tropaeolum majus [437], Tulipa sp.…”

Section: α-Carotenementioning

confidence: 99%

“…[366], Solanum lycopersicum 'M82' [5], Sophora japónica, Spathiphyllum montanum [366], Solanum lycopersicum, Solanum rantonnetii [366], Tabebuia chrysantha, Tagetes erecta [5], Lady Tagetes erecta, Tagetes patula [430], Tagetes patula 'Safari Tangerine' [5], Tecoma capensis, Trachelospermum jasminoides, Theretia peruviana, Thumbergia alata [5], Trachelospermum jasminoides, Trifolium cernnum, Trifolium hybridum, Tropaeolum majus [437], Tulipa sp. 'Golden Harvest' [5], Verbena × hybrida [366], Viola tricolor, Viola witrockiana 'Pansy' [307], Vitex agnus-costus [366], Warszewiczia coccinea, Wuonymus japonicus, Wuphorbia milii, Zantedeschia hybrid 'Florex Gold', Zinnia elegans [433], Zinnia elegans 'Dreamland Coral', Zinnia elegans 'Dreamland Red', and 'Dreamland Yellow ' [433].…”

Section: α-Carotenementioning

confidence: 99%

Exploring Plants with Flowers: From Therapeutic Nutritional Benefits to Innovative Sustainable Uses

Coyago-Cruz,

Moya,

Méndez

et al. 2023

Foods

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Flowers have played a significant role in society, focusing on their aesthetic value rather than their food potential. This study’s goal was to look into flowering plants for everything from health benefits to other possible applications. This review presents detailed information on 119 species of flowers with agri-food and health relevance. Data were collected on their family, species, common name, commonly used plant part, bioremediation applications, main chemical compounds, medicinal and gastronomic uses, and concentration of bioactive compounds such as carotenoids and phenolic compounds. In this respect, 87% of the floral species studied contain some toxic compounds, sometimes making them inedible, but specific molecules from these species have been used in medicine. Seventy-six percent can be consumed in low doses by infusion. In addition, 97% of the species studied are reported to have medicinal uses (32% immune system), and 63% could be used in the bioremediation of contaminated environments. Significantly, more than 50% of the species were only analysed for total concentrations of carotenoids and phenolic compounds, indicating a significant gap in identifying specific molecules of these bioactive compounds. These potential sources of bioactive compounds could transform the health and nutraceutical industries, offering innovative approaches to combat oxidative stress and promote optimal well-being.

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Spermine mitigates the adverse effects of water deficit by strengthening antioxidant enzymes and anthocyanin pathway-related gene expressions

Heydari

Raji

Nejad

et al. 2023

Industrial Crops and Products

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Carotenoid analysis and functional characterization of lycopene cyclases in Zinnia elegans L.

Cited by 4 publications

References 51 publications

Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60

Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60

Exploring Plants with Flowers: From Therapeutic Nutritional Benefits to Innovative Sustainable Uses

Spermine mitigates the adverse effects of water deficit by strengthening antioxidant enzymes and anthocyanin pathway-related gene expressions

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