Carvacrol-induced [Ca2+]i rise and apoptosis in human glioblastoma cells

Liang, Wei-Zhe; Lu, Cheng Hsien

doi:10.1016/j.lfs.2012.03.027

Cited by 70 publications

(45 citation statements)

References 39 publications

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“…Further compounds of essential oils investigated (eugenol and eucalyptol) failed to show any DNA protective effect. To sum up, the mild cytotoxic effect of carvacrol and thymol (IC≤ 50 ) on hepatoma and colonic cells had no DNA-damaging effect and even reduced the level of DNA lesions induced in these cells by an oxidative agent, and thus acted as antioxidants. It is evident that at IC≤ 50 the antioxidative activity of carvacrol and thymol is not accompanied by pro-oxidative effects.…”

Section: Discussionmentioning

confidence: 99%

“…Bactericidal effects of EOs occurred at many times lower concentrations Cytotoxic effects of EOs are attributed generally to their destructive effects on the cell membranes but other causes must also be considered. Liang and Lu [50] found that carvacrol Cytotoxic effects of EOs are attributed generally to their destructive effects on the cell membranes but other causes must also be considered. Liang and Lu [50] found that carvacrol at a concentration of 400- …”

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confidence: 99%

“…Liang and Lu [50] found that carvacrol Cytotoxic effects of EOs are attributed generally to their destructive effects on the cell membranes but other causes must also be considered. Liang and Lu [50] found that carvacrol at a concentration of 400- …”

mentioning

confidence: 99%

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Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Slameñová¹,

Horváthová²

2013

neo

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Flowers, berries, leaves, barks and roots of different plants have been used through the ages as a source of flavor in food and perfume preparations. The volatiles responsible for the flavor of botanicals can be extracted from the plant material as "essential oils" (EOs), called also volatile oils or ethereal oils. The term essential is intended to indicate that the oil is the fragrant essence of the plant from which it is extracted. EOs are constituted by hydrocarbons (monoterpenes and sesquiterpenes) and oxygenated compounds (alcohols, esters, ethers, aldehydes, ketones, lactones, phenols and phenol ethers). Of the numerous groups of naturally occurring compounds examined so far terpenes are known as fragrances and flavoring agents. The data reported in this review including the data obtained in our laboratory show that many of EOs exhibit a range of biological activities inclusive of antioxidative, anti-mutagenic and anti-carcinogenic activities. Most of them belong to phytochemicals with chemopreventive potential. On the other hand some herbal products can cause serious adverse effects. A complex research of toxic, genotoxic, anti-mutagenic and anti-carcinogenic effects of EOs is therefore very important.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Slameñová¹,

Horváthová²

2013

neo

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“…Carvacrol is commonly used in the food industry and in cosmetics, as preservative and antioxidant. Interestingly, it also possesses a myriad of pharmacological properties, such anti‐inflammatory, antibacterial, antitumor and antiplatelet ones …”

Section: Introductionmentioning

confidence: 99%

Voltage-operated potassium (Kv) channels contribute to endothelium-dependent vasorelaxation of carvacrol on rat aorta

Testai

Chericoni

Martelli

et al. 2016

Journal of Pharmacy and Pharmacology

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“…For this purpose, fura-2-loaded cells were challenged with 1 µM PMA, a PKC activator[44], in the presence of Ca2+ in the extracellular medium (Fig. 3A; n = 5 experiments/100 of 100 total cells studied).…”

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confidence: 99%

Melatonin induces the expression of Nrf2-regulated antioxidant enzymes via PKC and Ca2+ influx activation in mouse pancreatic acinar cells

Santofimia‐Castaño

Ruy

Garcia-Sanchez

et al. 2015

Free Radical Biology and Medicine

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The goal of this study was to evaluate the potential activation of the nuclear factor erythroid 2-related factor and the antioxidant-responsive element (Nrf2-ARE) signaling pathway in response to melatonin in isolated mouse pancreatic acinar cells. Changes in intracellular free Ca(2+) concentration were followed by fluorimetric analysis of fura-2-loaded cells. The activations of PKC and JNK were measured by Western blot analysis. Quantitative reverse transcription-polymerase chain reaction was employed to detect the expression of Nrf2-regulated antioxidant enzymes. Immunocytochemistry was employed to determine nuclear location of phosphorylated Nrf2, and the cellular redox state was monitored following MitoSOX Red-derived fluorescence. Our results show that stimulation of fura-2-loaded cells with melatonin (1 µM to 1 mM), in the presence of Ca(2+) in the extracellular medium, induced a slow and progressive increase of [Ca(2+)](c) toward a stable level. Melatonin did not inhibit the typical Ca(2+) response induced by CCK-8 (1 nM). When the cells were challenged with indoleamine in the absence of Ca(2+) in the extracellular solution (medium containing 0.5 mM EGTA) or in the presence of 1 mM LaCl(3), to inhibit Ca(2+) entry, we could not detect any change in [Ca(2+)](c). Nevertheless, CCK-8 (1 nM) was able to induce the typical mobilization of Ca(2+). When the cells were incubated with the PKC activator PMA (1 µM) in the presence of Ca(2+) in the extracellular medium, we observed a response similar to that noted when the cells were challenged with melatonin 100 µM. However, in the presence of Ro31-8220 (3 µM), a PKC inhibitor, stimulation of cells with melatonin failed to evoke changes in [Ca(2+)]c. Immunoblots, using an antibody specific for phospho-PKC, revealed that melatonin induces PKCα activation, either in the presence or in the absence of external Ca(2+). Melatonin induced the phosphorylation and nuclear translocation of the transcription factor Nrf2, and evoked a concentration-dependent increase in the expression of the antioxidant enzymes NAD(P)H-quinone oxidoreductase 1, catalytic subunit of glutamate-cysteine ligase, and heme oxygenase-1. Incubation of MitoSOX Red-loaded pancreatic acinar cells in the presence of 1 nM CCK-8 induced a statistically significant increase in dye-derived fluorescence, reflecting an increase in oxidation, that was abolished by pretreatment of cells with melatonin (100 µM) or PMA (1 µM). On the contrary, pretreatment with Ro31-8220 (3 µM) blocked the effect of melatonin on CCK-8-induced increase in oxidation. Finally, phosphorylation of JNK in the presence of CCK-8 or melatonin was also observed. We conclude that melatonin, via modulation of PKC and Ca(2+) signaling, could potentially stimulate the Nrf2-mediated antioxidant response in mouse pancreatic acinar cells.

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Carvacrol-induced [Ca2+]i rise and apoptosis in human glioblastoma cells

Cited by 70 publications

References 39 publications

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Voltage-operated potassium (Kv) channels contribute to endothelium-dependent vasorelaxation of carvacrol on rat aorta

Melatonin induces the expression of Nrf2-regulated antioxidant enzymes via PKC and Ca2+ influx activation in mouse pancreatic acinar cells

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