Cascaded filter deterministic lateral displacement microchips for isolation and molecular analysis of circulating tumor cells and fusion cells

Liu, Zongbin; Huang, Yuqing; Liang, Wenli; Bai, Jing; Feng, Hongtao; Fang, Zhihao; Tian, Geng; Zhu, Yanjuan; Zhang, Haibo; Wang, Yuanxiang; Liu, Aixue; Chen, Yan

doi:10.1039/d1lc00360g

Cited by 45 publications

(19 citation statements)

References 42 publications

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“…They achieved >85% capture efficiency of spiked breast cancer cell lines from whole blood at high flow rate (600 mL/h) [54]. DLD, combined with filter structures, has also been reported by Liu et al, leading to high separation efficiency (>96%) with high purity [55]. Recently, Au et al fabricated a two-stage DLD device to isolate intact CTC clusters [56].…”

Section: Deterministic Lateral Displacementmentioning

confidence: 88%

Microfluidic-Based Technologies for CTC Isolation: A Review of 10 Years of Intense Efforts towards Liquid Biopsy

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Roy

Deman

2022

IJMS

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The selection of circulating tumor cells (CTCs) directly from blood as a real-time liquid biopsy has received increasing attention over the past ten years, and further analysis of these cells may greatly aid in both research and clinical applications. CTC analysis could advance understandings of metastatic cascade, tumor evolution, and patient heterogeneity, as well as drug resistance. Until now, the rarity and heterogeneity of CTCs have been technical challenges to their wider use in clinical studies, but microfluidic-based isolation technologies have emerged as promising tools to address these limitations. This review provides a detailed overview of latest and leading microfluidic devices implemented for CTC isolation. In particular, this study details must-have device performances and highlights the tradeoff between recovery and purity. Finally, the review gives a report of CTC potential clinical applications that can be conducted after CTC isolation. Widespread microfluidic devices, which aim to support liquid-biopsy-based applications, will represent a paradigm shift for cancer clinical care in the near future.

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Section: Deterministic Lateral Displacementmentioning

confidence: 88%

Microfluidic-Based Technologies for CTC Isolation: A Review of 10 Years of Intense Efforts towards Liquid Biopsy

Descamps

Roy

Deman

2022

IJMS

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“…The test results are transmitted to the hospital and the user simultaneously ( Liang et al, 2019 ). In addition, in the field of analytical chemistry, there are also some paper-based POCT combined with electrochemical biosensors for the detection of secretions and biomarkers ( Gao et al, 2020 ; Li et al, 2020 ; Liu et al, 2019 , Liu et al, 2019 , Liu et al, 2021 , Liu et al, 2021 ). Paper-based microfluidic chips have great application potential in POCT field.…”

Section: Paper-based and Hybrid Microfluidic Chipsmentioning

confidence: 99%

Microfluidic technology and its application in the point-of-care testing field

Xie

Dai

Yang

2022

Biosensors and Bioelectronics: X

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Since the outbreak of the coronavirus disease 2019 (COVID-19), countries around the world have suffered heavy losses of life and property. The global pandemic poses a challenge to the global public health system, and public health organizations around the world are actively looking for ways to quickly and efficiently screen for viruses. Point-of-care testing (POCT), as a fast, portable, and instant detection method, is of great significance in infectious disease detection, disease screening, pre-disease prevention, postoperative treatment, and other fields. Microfluidic technology is a comprehensive technology that involves various interdisciplinary disciplines. It is also known as a lab-on-a-chip (LOC), and can concentrate biological and chemical experiments in traditional laboratories on a chip of several square centimeters with high integration. Therefore, microfluidic devices have become the primary implementation platform of POCT technology. POCT devices based on microfluidic technology combine the advantages of both POCT and microfluids, and are expected to shine in the biomedical field. This review introduces microfluidic technology and its applications in combination with other technologies.

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“…Although CTC counts in patient samples usually ranges between 1-1000/mL of blood (2500 mCTCs/mL were spiked here), the outstanding CTC/WBC ratio obtained after the MagPure chip paves the way for downstream single-cell studies. As an example, single-cell RNA sequencing can explore drug resistance mechanisms and help elucidate inter-and intrapatient heterogeneity 68 . Although it presents significant Please do not adjust margins Please do not adjust margins challenges including preservation of RNA quality and single-cell mRNA amplification 69 , it could fill the gap in personalized medicine approach.…”

Section: Lab On a Chip Accepted Manuscriptmentioning

confidence: 99%

MagPure chip: an immunomagnetic-based microfluidic device for high purification of circulating tumor cells from liquid biopsies

et al. 2022

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Cascaded filter deterministic lateral displacement microchips for isolation and molecular analysis of circulating tumor cells and fusion cells

Abstract: Precise isolation and analysis of circulating tumor cells (CTCs) from blood samples offer considerable potential for cancer research and personalized treatment. Currently, available CTCs isolation approaches remain challenging in the...

Cited by 45 publications

References 42 publications

Microfluidic-Based Technologies for CTC Isolation: A Review of 10 Years of Intense Efforts towards Liquid Biopsy

Microfluidic-Based Technologies for CTC Isolation: A Review of 10 Years of Intense Efforts towards Liquid Biopsy

Microfluidic technology and its application in the point-of-care testing field

MagPure chip: an immunomagnetic-based microfluidic device for high purification of circulating tumor cells from liquid biopsies

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