Case-Control Study of Platelet Glycoprotein Receptor Ib and IIb/IIIa Expression in Patients with Acute and Chronic Cerebrovascular Disease

Abstract

BackgroundAnimal models have been instrumental in defining thrombus formation, including the role of platelet surface glycoprotein (GP) receptors, in acute ischemic stroke (AIS). However, the involvement of GP receptors in human ischemic stroke pathophysiology and their utility as biomarkers for ischemic stroke risk and severity requires elucidation.AimsTo determine whether platelet GPIb and GPIIb/IIIa receptors are differentially expressed in patients with AIS and chronic cerebrovascular disease (CCD) compared with healthy volunteers (HV) and to identify predictors of GPIb and GPIIb/IIIa expression.MethodsThis was a case—control study of 116 patients with AIS or transient ischemic attack (TIA), 117 patients with CCD, and 104 HV who were enrolled at our University hospital from 2010 to 2013. Blood sampling was performed once in the CCD and HV groups, and at several time points in patients with AIS or TIA. Linear regression and analysis of variance were used to analyze correlations between platelet GPIb and GPIIb/IIIa receptor numbers and demographic and clinical parameters.ResultsGPIb and GPIIb/IIIa receptor numbers did not significantly differ between the AIS, CCD, and HV groups. GPIb receptor expression level correlated significantly with the magnitude of GPIIb/IIIa receptor expression and the neutrophil count. In contrast, GPIIb/IIIa receptor numbers were not associated with peripheral immune-cell sub-population counts. C-reactive protein was an independent predictor of GPIIb/IIIa (not GPIb) receptor numbers.ConclusionsPlatelet GPIb and GPIIb/IIIa receptor numbers did not distinguish between patient or control groups in this study, negating their potential use as a biomarker for predicting stroke risk.

Role of <i>ITGB3</i>, <i>GP1B1</i>, and <i>ITGA2</i> gene polymorphisms in platelet dysfunction in patients with COVID-19-associated lung damage

“…При гетерозиготном варианте С/Т экспрессия рецепторов GpIb/IX/V на тромбоцитах не так сильно выражена, однако в некоторых исследованиях выяв-лен повышенный риск тромбообразования у носителей этого варианта гена [16]. В ряде исследований показано, что носители аллеля С имеют повышенный риск коронарного тромбоза, ишемического инсульта и снижения возраста его наступления [17].…”

Platelet immunophenotyping in health and inherited bleeding disorders, a review and practical hints

“…Two techniques are available to study the CD41/CD61 complex, GPIIb-IIIa, at levels less than 5% of normal. This level is between 5 and 15% for type II, whereas type III is only a qualitative deficiency with variable levels of the surface integrin (Kraft, Drechsler, Gunreben, Heuschmann, & Kleinschnitz, 2015;Wagner et al, 1996).…”

Common Genetic Variants in Platelet Surface Receptors and its Association with Ischemic Stroke

“…A study in Chinese population was performed by Zhang et al who analyzed the above-mentioned polymorphism and observed that the frequency of D allele of VNTR was considerably higher in patients than in controls (Table 3) [28]. In contrary to these positive results, there are also a number of studies which show no association between afore mentioned GP1BB polymorphisms and ischemic stroke (Table 4) [18,61,66].…”

“…Even though several positive studies exist, there is also a number of minor controversies concerning the functional role of rs5918 in different populations, which do not support any association between rs5918 polymorphism and ischemic stroke [9,28,32,44,45,[60][61][62][63]. In an initially performed meta-analysis, which included 26 studies with five referring to rs5918 polymorphism, a dominant genetic model revealed no linkage with ischemic stroke [49].…”