Case-Control Study of the Association between Single Nucleotide Polymorphisms of Genes Involved in Xenobiotic Detoxification and Antioxidant Protection with the Long-Term Influence of Organochlorine Pesticides on the Population of the Almaty Region

Abstract

The association of genetic polymorphisms with the individual sensitivity of humans to the action of pesticide pollution is being actively studied in the world. The aim of this study was a molecular epidemiological analysis of candidate polymorphisms of genes involved in pesticide metabolism, detoxification, and antioxidant protection. Some of the selected polymorphisms also relate to susceptibility to cancer and cardiovascular, respiratory, and immune system diseases in individuals exposed to pesticides for a long time. For a case-control study of a unique cohort of people exposed to organochlorine pesticides for 10 years or more were chosen, a control cohort was selected that matched with the experimental group by the main population characteristics. PCR-PRLF and genome-wide microarray genotyping (GWAS) methods were used. We identified 17 polymorphisms of xenobiotic detoxification genes and 27 polymorphisms of antioxidant defense genes, which had a significantly high statistical association with the negative impact of chronic pesticide intoxication on human health. We also found 17 polymorphisms of xenobiotic detoxification genes and 12 polymorphisms of antioxidant defense genes that have a protective effect. Data obtained added to the list of potential polymorphisms that define a group at high risk or resistant to the negative effects of pesticides.

Genome-wide study of Almaty region people for a long time living in areas of pesticide contamination

“…Microarray SNP genotyping was performed on the Infinium Global Screening Array 24 v3.0 platform on the iScan system (Illumina, CA, USA) using the automated Infinium HTS protocol [9,10]. Detailed results of annotation of 654,270 SNP markers are presented in a previously published study [11]. Quality control was performed using PLINK v1.9 [12] to according to the standard algorithm.…”

Application of the switchSENSE® technology for real-time study of pesticides interaction with biological molecules

“…Despite their prohibition, residues of OCPs can still be found in the soil, water and food [1,2]. Furthermore, these pesticides have a chronic toxic effect on both animal and human population [3,4]. Nevertheless, the understanding of the physiological mechanisms by which OCPs are transported to various peripheral tissues and how these compounds exert harmful effects in the body is still not well understood.…”