Case of a stronger capability of maize seedlings to use ammonium being responsible for the higher 15N recovery efficiency of ammonium compared with nitrate

Zhang, Hao Qing; Zhao, Xue Qiang; Chen, Yi Ling; Zhang, Ling Yu; Shen, Ren Fang

doi:10.1007/s11104-019-04087-w

Cited by 36 publications

(24 citation statements)

References 65 publications

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“…The predominant N sources of cucumber are NO 3 − -N and NH 4 + -N. Compared with a single NO 3 − -N source or a single NH 4 + -N source, a compound N source is more conducive to N absorption and plant growth [ 31 ]. Plant preference for NO 3 − -N or NH 4 + -N is associated with species and is influenced by environmental conditions and growth stage [ 58 – 60 ]. Kant [ 61 ] considered that improvement of nitrate uptake and transport would enhance plant growth, resulting in improved crop yields.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it’s transportation

et al. 2021

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Background Low temperature severely depresses the uptake, translocation from the root to the shoot, and metabolism of nitrate and ammonium in thermophilic plants such as cucumber (Cucumis sativus). Plant growth is inhibited accordingly. However, the availability of information on the effects of low temperature on nitrogen transport remains limited. Results Using non-invasive micro-test technology, the net nitrate (NO3−) and ammonium (NH4+) fluxes in the root hair zone and vascular bundles of the primary root, stem, petiole, midrib, lateral vein, and shoot tip of cucumber seedlings under normal temperature (NT; 26 °C) and low temperature (LT; 8 °C) treatment were analyzed. Under LT treatment, the net NO3− flux rate in the root hair zone and vascular bundles of cucumber seedlings decreased, whereas the net NH4+ flux rate in vascular bundles of the midrib, lateral vein, and shoot tip increased. Accordingly, the relative expression of CsNRT1.4a in the petiole and midrib was down-regulated, whereas the expression of CsAMT1.2a–1.2c in the midrib was up-regulated. The results of 15N isotope tracing showed that NO3−-N and NH4+-N uptake of the seedlings under LT treatment decreased significantly compared with that under NT treatment, and the concentration and proportion of both NO3−-N and NH4+-N distributed in the shoot decreased. Under LT treatment, the actual nitrate reductase activity (NRAact) in the root did not change significantly, whereas NRAact in the stem and petiole increased by 113.2 and 96.2%, respectively. Conclusions The higher net NH4+ flux rate in leaves and young tissues may reflect the higher NRAact in the stem and petiole, which may result in a higher proportion of NO3− being reduced to NH4+ during the upward transportation of NO3−. The results contribute to an improved understanding of the mechanism of changes in nitrate transportation in plants in response to low-temperature stress.

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Section: Discussionmentioning

confidence: 99%

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it’s transportation

et al. 2021

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“…The 15 N fertilizer-derived N recovery in rice plants and soils was computed according to Zhang et al [ 55 ]. Loss of N derived from 15 N-labeled fertilizer was the difference between the total fertilizer N applied and the sum of the 15 N fertilizer-derived N recovery in rice plants and the soil N pool.…”

Section: Methodsmentioning

confidence: 99%

Improved Root Growth by Liming Aluminum-Sensitive Rice Cultivar or Cultivating an Aluminum-Tolerant One Does Not Enhance Fertilizer Nitrogen Recovery Efficiency in an Acid Paddy Soil

Zhang

Zhao

Chen

et al. 2020

Plants

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The root is the main site of nitrogen (N) acquisition and aluminum (Al) toxicity. The objective of this study is to investigate whether liming and cultivation of an Al-tolerant rice (Oryza sativa L.) cultivar can improve root growth, thereby increasing N acquisition by rice plants in acid paddy soil. Two rice cultivars (‘B690’, Al-sensitive, and ‘Yugeng5’, Al-tolerant) were cultivated with 15N-labeled urea, and with or without lime in an acid paddy soil (pH 4.9) in pots. We examined root and shoot growth, soil pH, soil exchangeable Al, N uptake, 15N distribution in plant-soil system, and fertilizer N recovery efficiency. Results showed that liming improved the root growth of ‘B690’ by decreasing soil exchangeable Al concentrations, in both N-limited and N-fertilized soils. Liming enhanced the N uptake of ‘B690’ only in the absence of N fertilizer. The root weight of ‘Yugeng5’ was greater than that of ‘B690’ without lime, but the two cultivars showed similar N uptake. The fertilizer N recovery efficiency and N loss did not differ significantly between limed and non-limed conditions, or between the two rice cultivars. Thus, liming an Al-sensitive rice cultivar and cultivating an Al-tolerant one improves root growth, but does not enhance fertilizer N recovery efficiency in the present acid paddy soil.

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“…Despite extensive research conducted over past decades, the concept of preference remains an enigma, a terminology in common usage, yet without definition or clarity. Nevertheless, interest persists, as evidenced by recent literature (e.g., Boschiero, Mariano, & Trivelin, 2018;Cui et al, 2017;Daryanto et al, 2019;Iqbal et al, 2020;Liu et al, 2019;Zhang, Zhao, Chen, Zhang, & Shen, 2019). At first glance, the solution to this question may appear to be straightforward.…”

Section: Introductionmentioning

confidence: 99%

On inorganic N uptake by vascular plants: Can ¹⁵N tracer techniques resolve the NH₄⁺ versus NO₃⁻ “preference” conundrum?

Chalk

2020

European J Soil Science

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The relative uptake by plants of the two ionic nitrogen (N) forms, ammonium (NH4+) and nitrate (NO3−), has been the subject of much interest during the past 50 years, resulting in a considerable scientific literature. The general idea is that plants have choice, resulting in preference for either one mineral N form or the other. Unfortunately, there is no specific definition of preference or agreement on how it should be measured. In this review, we critically examine the alternative techniques that have been used to measure the relative uptake of NH4+ and NO3− by plants, including those based on unlabelled sources, 15N‐enriched mineral N forms and variations in the 15N natural abundance of mineral N sources. The main difficulty with using unlabelled N is the antecedent N in plant tissue prior to the imposition of treatments. Although 15N‐enrichment overcomes this obstacle, it is nevertheless difficult to separate uptake as 15NH4+, uptake as 15NO3− and uptake as 15NO3− derived from the nitrification of 15NH4+. With 15N natural abundance, isotopic fractionation during plant uptake complicates the interpretation of data. There is increasing evidence that plants exhibit flexibility or plasticity with respect to the use of mineral N forms rather than preference.Highlights The concept of plant preference for NH4+ or NO3− forms of mineral N is examined Experiments using 15N‐enrichment and 15N natural abundance are reviewed The direct uptake of 15NO3− is confounded with the uptake of 15NO3− derived from nitrified 15NH4+ Plants exhibit plasticity rather than preference in the acquisition of ammonium and nitrate

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Case of a stronger capability of maize seedlings to use ammonium being responsible for the higher 15N recovery efficiency of ammonium compared with nitrate

Cited by 36 publications

References 65 publications

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it’s transportation

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it’s transportation

Improved Root Growth by Liming Aluminum-Sensitive Rice Cultivar or Cultivating an Aluminum-Tolerant One Does Not Enhance Fertilizer Nitrogen Recovery Efficiency in an Acid Paddy Soil

On inorganic N uptake by vascular plants: Can ¹⁵N tracer techniques resolve the NH₄⁺ versus NO₃⁻ “preference” conundrum?

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Case of a stronger capability of maize seedlings to use ammonium being responsible for the higher 15N recovery efficiency of ammonium compared with nitrate

Cited by 36 publications

References 65 publications

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it’s transportation

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it’s transportation

Improved Root Growth by Liming Aluminum-Sensitive Rice Cultivar or Cultivating an Aluminum-Tolerant One Does Not Enhance Fertilizer Nitrogen Recovery Efficiency in an Acid Paddy Soil

On inorganic N uptake by vascular plants: Can 15N tracer techniques resolve the NH4+ versus NO3− “preference” conundrum?

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On inorganic N uptake by vascular plants: Can ¹⁵N tracer techniques resolve the NH₄⁺ versus NO₃⁻ “preference” conundrum?