Casein Kinase 2 Interacting Protein-1 Suppresses Glioma Cell Proliferation via Regulating the AKT/GSK3<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>β</mml:mi></mml:mrow></mml:math>/<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mrow><mml:mi>β</mml:mi></mml:mrow></mml:math>-Catenin Pathway

Xi, Yanguo; Ren, Deng-Peng; Jin, Jing-Yun; Zhu, Lei; Yi, Tailong; Shao, Xiaoqiu; Sun, Shengkai; Zhang, Wenbin; Shixiang, Cheng

doi:10.1155/2019/5653212

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…2 d), meaning si-HOXA-AS2 was successfully transfected into U87 and U251 cells. CCK-8, BrdU and flow cytometry are commonly used to evaluate the proliferation and apoptosis of cancer cells [ 27 , 28 ]. Our CCK-8 assay outcome indicated that compared with the blank group, silencing HOXA-AS2 inhibited cell viability after U87 and U251 cells were transfected with si-HOXA-AS2 for 48 and 72 h (Fig.…”

Section: Resultsmentioning

confidence: 99%

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LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

Shou¹,

Gao²,

Cheng³

et al. 2021

Cancer Cell Int

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Background LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. Methods qRT-PCR analysis was performed to detect the expression of lncRNA, miRNA and mRNA in glioblastoma tissues and cells. Dual-luciferase assay, RIP assay and RNA pull-down assay were later carried out to reveal the interactions among HOXA-AS2, miR-885-5p and RBBP4. After that, CCK-8 assay, BrdU assay, nude mice xenografting assay, western blot assay, and flow cytometry were carried out to analyze the effect of the HOXA-AS2/miR-885-5p/RBBP4 axis on glioblastoma samples. Results HOXA-AS2 and RBBP4 were found to be overexpressed in glioblastoma. Experimental results showed that HOXA-AS2 and RBBP4 contributed to the tumorigenesis of glioblastoma cells. However, miR-885-5p was observed to be downregulated in glioblastoma. Findings also indicated that HOXA-AS2 could negatively regulate miR-885-5p, thereby enhancing RBBP4 expression. Conclusion Overall, HOXA-AS2 promoted the tumorigenesis of glioblastoma by targeting and regulating miR-885-5p to induce the expression of RBBP4.

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Section: Resultsmentioning

confidence: 99%

“…CCK-8 assay, BrdU assay and flow cytometry are commonly used to study the proliferation and apoptosis of cancer cells [ 27 , 28 ]. Cell adhesion assay is also performed to assess tumor development [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

Shou¹,

Gao²,

Cheng³

et al. 2021

Cancer Cell Int

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“…The results of our qRT-PCR analysis showed that HOXA-AS2 silencing signi cantly reduced HOXA-AS2 expression by 70% compared to the blank group ( Figure 2D), meaning si-HOXA-AS2 was successfully transfected into U87 and U251 cells. CCK-8, BrdU and ow cytometry are commonly used to evaluate the proliferation and apoptosis of cancer cells [27,28]. Our CCK-8 assay outcome indicated that compared with the blank group, silencing HOXA-AS2 inhibited cell viability after U87 and U251 cells were transfected with si-HOXA-AS2 for 48 and 72 hours ( Figure 2E).…”

Section: Effects Of Si-hoxa-as2 On Glioblastoma Samplesmentioning

confidence: 87%

“…CCK-8 assay, BrdU assay and ow cytometry are commonly used to study the proliferation and apoptosis of cancer cells [27,28]. Cell adhesion assay is also performed to assess tumor development [29,30].…”

Section: Discussionmentioning

confidence: 99%

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

Shou

Gao

Cheng

et al. 2020

Preprint

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Background: LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. Methods: qRT-PCR analysis was performed to detect the expression of lncRNA, miRNA and mRNA in glioblastoma tissues and cells. Dual-luciferase assay, RIP assay and RNA pull-down assay were later carried out to reveal the interactions among HOXA-AS2, miR-885-5p and RBBP4. After that, CCK-8 assay, BrdU assay, nude mice xenografting assay, western blot assay, and flow cytometry were carried out to analyze the effect of the HOXA-AS2/miR-885-5p/RBBP4 axis on glioblastoma samples. Results: HOXA-AS2 and RBBP4 were found to be overexpressed in glioblastoma. Experimental results showed that HOXA-AS2 and RBBP4 contributed to the tumorigenesis of glioblastoma cells. However, miR-885-5p was observed to be downregulated in glioblastoma. Findings also indicated that HOXA-AS2 could negatively regulate miR-885-5p, thereby enhancing RBBP4 expression. Conclusion: Overall, HOXA-AS2 promoted the tumorigenesis of glioblastoma by targeting and regulating miR-885-5p to induce the expression of RBBP4.

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“…4 Currently, research suggests that the CKIP-1 gene might play an important role in the occurrence and development of several malignancies. 5–9 However, the specific role of CKIP-1 in GC remains unclear. Previous research by the current authors demonstrated that the CKIP-1 gene was related to GC cell differentiation and involved in the proliferation, migration and invasion of GC, acting as a tumour suppressor gene.…”

Section: Introductionmentioning

confidence: 99%

High expression of the CKIP-1 gene might promote apoptosis through downregulation of the Ras/ERK signalling pathway in the intestinal type of gastric cancer

Cao

et al. 2020

J Int Med Res

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Objective: To investigate the effect of the casein kinase 2 interacting protein 1 (CKIP-1) on the apoptosis of the intestinal type of gastric cancer (GC). Methods: The levels of CKIP-1 protein and the rates of apoptosis were measured in tissue samples of the intestinal type of GC and human GC cell lines. The rate of apoptosis and the protein levels of B cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), cleaved cysteinyl aspartate specific protease 3 (cleaved caspase-3), cleaved caspase-9, rat sarcoma (Ras), extracellular signal-regulated kinase 1 and 2 (ERK1/2) and phosphorylated extracellular signal-regulated kinase 1 and 2 (p-ERK1/2) were analysed in SGC7901 cells expressing CKIP-1 short hairpin RNA (shRNA; knockdown) and SGC7901 cells overexpressing CKIP-1. Results: The levels of CKIP-1 protein were significantly lower in the intestinal type of GC tissues compared with the samples of intestinal metaplasia. Both the levels of CKIP-1 protein and the levels of apoptosis decreased gradually with decreasing cell differentiation in the intestinal type of GC tissue and cell lines; and they were positively correlated. In the CKIP-1 shRNA group, the rate of apoptosis and the levels of Bax, cleaved caspase-3 and cleaved caspase-9 were decreased; and the levels of Bcl-2, Ras and the ratio of p-ERK/ERK were increased, compared with the control group. Opposite results were observed in the CKIP-1 overexpression group.

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Casein Kinase 2 Interacting Protein-1 Suppresses Glioma Cell Proliferation via Regulating the AKT/GSK3β/β-Catenin Pathway

Cited by 8 publications

References 24 publications

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

High expression of the CKIP-1 gene might promote apoptosis through downregulation of the Ras/ERK signalling pathway in the intestinal type of gastric cancer

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