Caspase‐3: Structure, function, and biotechnological aspects

Asadi, Mohammad; Taghizadeh, Saeed; Kaviani, Elina; Vakili, Omid; Taheri‐Anganeh, Mortaza; Tahamtan, Mahshid; Savardashtaki, Amir

doi:10.1002/bab.2233

Cited by 245 publications

(132 citation statements)

References 90 publications

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“…Based on our results of TEM and ERG, we expected to see reduced apoptosis in the WIF1‐treated OIR mice retina. Figure 7A showed that WIF1 overexpression strongly suppressed the levels of caspase‐3 and cleaved capsase‐3, which suggested reduced retinal apoptosis 27 . In addition, we employed Terminal deoxynucleotidyl transferase (TdT) dUTP Nick‐End Labeling (TUNEL) staining to evaluate apoptosis in retinal cells.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 7A showed that WIF1 overexpression strongly suppressed the levels of caspase-3 and cleaved capsase-3, which suggested reduced retinal apoptosis. 27 In addition, we employed Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) staining to evaluate apoptosis in retinal cells. We revealed a 75% decreased in apoptotic nuclei in the retina of LV-WIF1-treated OIR retina, compared to controls (Figure 7B).…”

Section: Wif1 Decreased Hypoxia-driven Photoreceptor Apoptosis By Red...mentioning

confidence: 99%

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Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen‐induced retinopathy mouse model

Tan

Chen

et al. 2022

BioFactors

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Retinal neovascularization (RNV) associated diseases typically exhibit pathological neovascularization and neurodegeneration. Wnt inhibitor factor 1 (WIF1) is a secreted Wnt antagonist that regulates angiogenesis. However, the significance of WIF1 in RNV associated disease has not been explicitly tested. In our study, we found that the WIF1 expressions were strongly downregulated in the vitreous of proliferative diabetic retinopathy (PDR) and retinopathy of prematurity (ROP). Similarly, retinal WIF1 expression was significantly downregulated in OIR mice, relative to normal mice at P17. After injection of WIF1 overexpression lentivirus into the vitreous of OIR mice, overexpressing WIF1 in OIR mice vitreous strongly reduced avascular areas and neovascular tufts, increased vessel branches, raised a‐, b‐waves and oscillatory potentials amplitudes on ERG, increased retinal thickness and the number of synapses in retina, normalized the Golgi, mitochondria, and outer segments of photoreceptors. Furthermore, overexpression WIF1 suppressed expressions of β‐catenin, vascular endothelial growth factor (VEGF), p‐AKT and p‐ERK, reduced retinal reactive oxygen species (ROS) and 4‐HNE levels, improved autophagic flux, and mitigated apoptosis. In summary, WIF1 plays a key role in alleviating angiogenesis and in improving visual function in OIR mice by suppressing the Wnt/β‐catenin‐VEGF signaling pathway and ROS levels. WIF1 is an excellent candidate for targeted therapy against RNV associated diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Wif1 Decreased Hypoxia-driven Photoreceptor Apoptosis By Red...mentioning

confidence: 99%

Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen‐induced retinopathy mouse model

Tan

Chen

et al. 2022

BioFactors

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“…These data indicate that PI3Kγ deficiency inhibits apoptotic cell death in the kidney following IRI. Caspase 3 is the final effector caspase that results in apoptotic cell death [ 23 , 24 ]. Therefore, the effect of PI3Kγ deficiency on caspase 3 protein expression was examined in the kidney.…”

Section: Resultsmentioning

confidence: 99%

Phosphoinositide 3 Kinase γ Plays a Critical Role in Acute Kidney Injury

Jin

Chu

Sun

et al. 2022

Cells

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Inflammatory cells contribute to the pathogenesis of renal ischemia-reperfusion injury (IRI). However, the signaling mechanisms underlying the infiltration of inflammatory cells into the kidney are not well understood. In this study, we examined the effects of phosphoinositide 3 kinase γ (PI3Kγ) on inflammatory cells infiltration into the kidney in response to ischemia-reperfusion injury. Compared with wild-type mice, PI3Kγ knockout mice displayed less IRI in the kidney with fewer tubular apoptotic cell. Furthermore, PI3Kγ deficiency decreased the number of infiltrated neutrophils, macrophages, and T cells in the kidney, which was accompanied by a decrease in the expression of pro-inflammatory cytokines in the kidney. Moreover, wild-type mice treated with AS-605240, a selective PI3Kγ inhibitor, displayed less tubular damage, accumulated fewer inflammatory cells, and expressed less proinflammatory molecules in the kidney following IRI. These results demonstrate that PI3Kγ has a critical role in the pathogenesis of kidney damage in IRI, indicating that PI3Kγ inhibition may serve as a potential therapeutic strategy for the prevention of ischemia-reperfusion-induced kidney injury.

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“…Remarkably, the glutathione S-transferase activity in the anterior portion of P. lividus intestine has been employed as biomarker of environmental contamination, revealing an important role in the homeostasis of this tissue [69]. Finally, CASP3 gene encodes for a cysteine-aspartic acid protease (caspase) that has a crucial role in the execution-phase of apoptosis [70]. This protein has been described as an essential element if the innate immunity of sea urchins [64].…”

Section: Discussionmentioning

confidence: 99%

De novo Assembly and Analysis of Tissue-Specific Transcriptomes of the Edible Red Sea Urchin Loxechinus albus Using RNA-Seq

Antiqueo

Zuloaga

Bastías-Molina

et al. 2021

Biology

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Edible red sea urchin (Loxechinus albus) is an endemic echinoderm species of the Chilean coasts. The worldwide demand for high-quality gonads of this species has addressed the depletion of its natural populations. Studies on this sea urchin are limited, and genomic information is almost nonexistent. Hence, generate a transcriptome is crucial information that will considerably enrich molecular data and promote future findings for the L. albus aquaculture. Here, we obtained transcriptomic data of the edible red sea urchin by Illumina platform. Total RNA was extracted from gonads, intestines, and coelomocytes of juvenile urchins, and samples were sequenced using MiSeq Illumina technology. A total of 91,119,300 paired-end reads were de novo assembled, 185,239 transcripts produced, and a reference transcriptome created with 38.8% GC content and an N50 of 1769 bp. Gene ontology analysis revealed notable differences in the expression profiles between gonads, intestines, and coelomocytes, allowing the detection of transcripts associated with specific biological processes and KEGG pathways. These data were validated using 12 candidate transcripts by real-time qPCR. This dataset will provide a valuable molecular resource for L. albus and other species of sea urchins.

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Caspase‐3: Structure, function, and biotechnological aspects

Cited by 245 publications

References 90 publications

Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen‐induced retinopathy mouse model

Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen‐induced retinopathy mouse model

Phosphoinositide 3 Kinase γ Plays a Critical Role in Acute Kidney Injury

De novo Assembly and Analysis of Tissue-Specific Transcriptomes of the Edible Red Sea Urchin Loxechinus albus Using RNA-Seq

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