Caspase-8 has an essential role in resveratrol-induced apoptosis of rheumatoid fibroblast-like synoviocytes

Byun, Hee Sun; Song, Junmin; Kim, Y.-R.; Piao, Longzhu; Won, Minho; Park, K. A.; Choi, Byung-Lyul; Lee, H.; Hong, Ji‐Young; Park, J.; Seok, Jeong Ho; Lee, Y. J.; Kang, Seong Wook; Hur, Gang Min

doi:10.1093/rheumatology/kem368

Cited by 66 publications

(37 citation statements)

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“…This confirms that sirtuin 1 is required for resveratrol-induced MH7A cell apoptosis. Findings by Byun et al (2008) suggest that resveratrol induces apoptosis in fibroblast-like synoviocytes derived from patients with rheumatoid arthritis by activating caspase-8 as a primary target, which cleaves Bid, causing mitochondrial damage that triggers activation of caspase-9 and the effector caspase-3, without affecting the levels of Bax, Bcl-X L , and Bcl-2. This observation contrasts with our finding that resveratrol does not activate caspase-8 in MH7A cells (Nakayama et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Resveratrol: A Candidate Drug for Treating Rheumatoid Arthritis

Nishizaki¹,

Kanno²

2012

Rheumatoid Arthritis - Treatment

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Section: Discussionmentioning

confidence: 99%

Resveratrol: A Candidate Drug for Treating Rheumatoid Arthritis

Nishizaki¹,

Kanno²

2012

Rheumatoid Arthritis - Treatment

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“…On the other hand, in rheumatoid fibroblast-like synoviocytes resveratrol induced programmed cell death but the levels of Bax and Bcl-2 were not changed. Instead, resveratrol triggered the mitochondria-related apoptotic pathway by activating Bid cleavage via Caspase-8 [28]. Despite such inconsistencies most studies support the idea that above a certain threshold level resveratrol induces apoptosis and stimulates apoptosis-related genes characterizing resveratrol as an anti-cancer reagent [19,29,30].…”

Section: Discussionmentioning

confidence: 99%

Effect of Resveratrol on the Development of Porcine Embryos Produced In Vitro

Lee

Wang

Chaille

et al. 2010

Journal of Reproduction and Development

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Abstract. The effects of resveratrol (a phytoalexin with a wide variety of pharmacological activities) on pig embryos produced by parthenogenesis and/or in vitro fertilization have been investigated. First, parthenogenetic embryos were generated and cultured in PZM-3 medium supplemented with various amounts of resveratrol (0, 0.05, 0.1, 0.5, 1.0 and 25 μM final concentrations). In the presence of 0.5 μM resveratrol a significantly higher percentage of parthenogenetic embryos reached the blastocyst stage by day 7 compared to non-treated control (43.5 ± 6.3% vs. 33.0 ± 5.4%; P<0.05). The total cell number of blastocysts also increased as a result of incubation with 0.5 μM resveratrol; the difference was statistically significant between treated and non-treated embryos on day 5 of culture (35.8 ± 0.9 vs. 32.1 ± 1.1; P<0.05). Resveratrol incubation affected the expression levels of apoptosis-related genes in parthenogenetic blastocysts: the level of Bax transcripts was similar but lower expression of Bcl-2 and Caspase-3 was observed in embryos treated with 0.5 μM resveratrol when compared to control blastocysts (P<0.05). The results of the TUNEL assay were similar in blastocysts developing with or without resveratrol supplementation. In addition, when embryos produced by in vitro fertilization were incubated with 0.5 μM resveratrol, the treatment led to higher frequencies of blastocyst formation (8.6% vs. 13.3%) and elevated total cell numbers (37.1 ± 2.4 vs. 43.2 ± 1.7) by the end of the 7-day culture period (P<0.05). The results indicate that 0.5 μM resveratrol during culture has a positive effect on early embryonic development of porcine embryos. Key words: Development, Embryo, Pig, Resveratrol (J. Reprod. Dev. 56: [330][331][332][333][334][335] 2010) mbryo culture is valuable in the study of preimplantation embryonic development and is indispensable for the in vitro production of transferable embryos. In spite of significant improvements in the culture conditions, the development of in vitro-produced embryos is still suboptimal [1]. Because the preimplantation embryo is extremely sensitive to environmental factors, deficiencies in culture conditions often lead to aberrant embryo development that manifest in lower frequency of blastocyst formation and lower cell numbers and can affect fetal as well as postnatal life [2]. Modifications in the culture system can potentially improve the development of the cultured embryos.Resveratrol (3,4',5-trihydroxystilbene) is a phytoalexin identified in more than 70 plant species including grapes, plums, and peanuts [3]. Its biological function is to protect the plant in case of a parasitic attack or environmental stress [4]. Originally, the search for plant-derived compounds with pharmacological properties identified resveratrol as a blocker of enzymes involved in arachidonate metabolism [5] and later, an inhibitor of partially purified kinases [6]. Based on its ability to block the cyclooxygenase COX-1 resveratrol was suggested to have anticancer properties and it was demo...

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“…Rho123 was used to analyze the mitochondrial membrane potential (∆Ψm) [28]. To this end, MCF-7/ADR cells were seeded into 6-well plates and treated with various concentrations of Ac-aCD NP for 24 h. Then, cells were trypsinized, washed, and incubated with 10 µM of Rho123 in PBS at 37ºC for 30 min.…”

Section: Measurement Of the Mitochondrial Membrane Potentialmentioning

confidence: 99%