Caspofungin modulates in vitro adherence of Candida albicans to plastic coated with extracellular matrix proteins

Abstract

This study demonstrated the anti-adherent activity of caspofungin but indicated a reduced effect in the case of in vitro fluconazole resistance. These results indicated a possible relationship between the efficiency of caspofungin to inhibit the first step of the development of C. albicans biofilm and the resistance of C. albicans to fluconazole in vitro.

Candida albicansCell Wall Proteins

“…However, the heterozygous deletion had no effect on the induction of the low-affinity receptor by hemoglobin. This study and that of Soustre et al (358), also discussed elsewhere, report opposite effects of caspofungin treatment on binding to fibronectin. In both studies, organisms were grown in yeast nitrogen base with glucose (2% [111 mM] in one case and 50 mM in the other).…”

“…In both studies, organisms were grown in yeast nitrogen base with glucose (2% [111 mM] in one case and 50 mM in the other). There is a large difference in the caspofungin MICs of 625 ng/ml (293) and 8 ng/ml (358) but less difference in the amounts of caspofungin used, as the former used 10 ng/ml and the latter used MIC/2. One study used soluble fibronectin (293) and the other immobilized fibronectin (358); however, with the high-affinity receptor, maximal activity was found with the gelatin/collagen binding domain fragment in both soluble and immobilized assays.…”

“…Several studies in recent years examined the effect of pretreatment with subinhibitory drug concentrations on adherence to various host ligands. Imbert and colleagues (47,153,358) examined adherence to uncoated polystyrene, fibrinogen, fibronectin, laminin, gelatin, collagen type IV, and ECM (a mixture of laminin, collagen type IV, entactin, and heparan sulfate proteoglycan) for yeast cells treated with one-fourth the MIC. Treatment with flucytosine had no effect.…”

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

“…that the drug concentrations required for reducing fungal metabolic activity by half had to be five to eight times higher in biofilms compared with planktonic cells, leading to at least a 30-fold increase in MIC (Mathé & van Dijck, 2013). Soustre et al (2004) showed that the same subMIC of caspofungin as that used in our research (MIC/2) decreased C. albicans adhesion, although mainly in the case of strains susceptible to fluconazole. The authors explained this phenomenon by an original mechanism of caspofungin action.…”

“…The authors explained this phenomenon by an original mechanism of caspofungin action. Caspofungin belongs to echinocandins -cyclic lipopeptides interfering with cell wall biosynthesis by the inhibition of 1,3-b-glucan synthase (Cannon et al, 2009;Rajeshkumar & Sundararaman, 2012;Soustre et al, 2004). Therefore, its influence on the yeast cell wall may lead to a disturbance in the expression of some cellular adhesins.…”