Cassettes for seed-specific expression tested in transformed embryogenic cultures of soybean

Cho, Myeong Je; Widholm, Jack M.; Vodkin, Lila O.

doi:10.1007/bf02670904

Cited by 42 publications

(23 citation statements)

References 20 publications

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“…Expression was driven by the soybean lectin promoter with either an in-frame soybean lectin signal sequence directing the enzyme to the protein storage vacuole (PSV; VAPPA) or with an ATG replacing the lectin signal sequence (Cho et al, 1995). This latter construct, CAPPA, was predicted to encode a cytoplasmic phytase.…”

Section: Soybean Seeds Expressing a Recombinant Phytasementioning

confidence: 99%

“…We chose APPA, a periplasmic phytase encoded by the Escherichia coli appA gene (Dassa et al, 1990;Greiner et al, 1993). To improve APPA accessibility to seed PA, we employed a construct (Cho et al, 1995) to direct APPA to the vacuole (Coello et al, 2001). While significant reductions in seed PA were observed in two independent transgenic lines, there was not a compensatory increase in Pi.…”

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confidence: 99%

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Quantitative Conversion of Phytate to Inorganic Phosphorus in Soybean Seeds Expressing a Bacterial Phytase

et al. 2007

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Phytic acid (PA) contains the major portion of the phosphorus in the soybean (Glycine max) seed and chelates divalent cations. During germination, both minerals and phosphate are released upon phytase-catalyzed degradation of PA. We generated a soybean line (CAPPA) in which an Escherichia coli periplasmic phytase, the product of the appA gene, was expressed in the cytoplasm of developing cotyledons. CAPPA exhibited high levels of phytase expression, $90% reduction in seed PA, and concomitant increases in total free phosphate. These traits were stable, and, although resulted in a trend for reduced emergence and a statistically significant reduction in germination rates, had no effect on the number of seeds per plant or seed weight. Because phytate is not digested by monogastric animals, untreated soymeal does not provide monogastrics with sufficient phosphorus and minerals, and PA in the waste stream leads to phosphorus runoff. The expression of a cytoplasmic phytase in the CAPPA line therefore improves phosphorus availability and surpasses gains achieved by other reported transgenic and mutational strategies by combining in seeds both high phytase expression and significant increases in available phosphorus. Thus, in addition to its value as a high-phosphate meal source, soymeal from CAPPA could be used to convert PA of admixed meals, such as cornmeal, directly to utilizable inorganic phosphorus.

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Section: Soybean Seeds Expressing a Recombinant Phytasementioning

confidence: 99%

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confidence: 99%

Quantitative Conversion of Phytate to Inorganic Phosphorus in Soybean Seeds Expressing a Bacterial Phytase

et al. 2007

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“…Soybean (Glycine max) is an important worldwide crop, and an important source of vegetable oil and protein (Cho et al, 1995). There is a large interest in improving soybean quality and production through genetic engineering (Wang and Xu, 2008).…”

Section: Introductionmentioning

confidence: 99%

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco

Cui

Chen

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13-and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.

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“…The concept of a soy-derived pharmaceutical was tested back in 1995 when Cho et al, developed a transformation expression cassette using a soybean seed-specific lectin promoter to test for potential expression of the β-glucuronidase reporter gene [60]. This same expression cassette was used to produce bovine β-casein in soybean which accumulated to 0.1-0.4% of seed total soluble protein (TSP) [61].…”

Section: Soybeans As Bioreactors For Pharmaceuticalsmentioning

confidence: 99%

Advancements in Transgenic Soy: From Field to Bedside

Hudson¹,

Lambirth²,

Bost³

et al. 2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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Cassettes for seed-specific expression tested in transformed embryogenic cultures of soybean

Cited by 42 publications

References 20 publications

Quantitative Conversion of Phytate to Inorganic Phosphorus in Soybean Seeds Expressing a Bacterial Phytase

Quantitative Conversion of Phytate to Inorganic Phosphorus in Soybean Seeds Expressing a Bacterial Phytase

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco

Advancements in Transgenic Soy: From Field to Bedside

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