Cat3, a third gene locus coding for a tissue-specific catalase in maize: Genetics, intracellular location, and some biochemical properties

Scandalios, John G.; Tong, Wu-Fu; Roupakias, D. G.

doi:10.1007/bf00268443

Cited by 91 publications

(55 citation statements)

References 19 publications

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“…However, the enzymes from microorganisms can utilize o-dianisidine in the peroxidatic reaction (10) whereas EP-CAT from tobacco cannot (our unpublished data). In maize, EP-CAT is the product of the Cat3 gene (14,15). In other plant species such as tobacco (4,5) and barley the evidence for separate genetic control is indirect.…”

Section: Discussionmentioning

confidence: 99%

Enhanced-Peroxidatic Activity in Specific Catalase Isozymes of Tobacco, Barley, and Maize

Havir

McHale²

1989

Plant Physiol.

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Separation of catalase isozymes from leaf extracts of three diverse plant species (Nicotiana sylvestris, Zea mays, Hordeum vulgare L.) revealed a distinct isozyme with enhanced peroxidatic activity (30-, 70-, 28-fold over typical catalase, respectively) which constituted 10 to 20% of the total catalase activity. In maize this isozyme is the product of the Cat3 gene, which is expressed only in mesophyll cells (AS Tsaftaris, AM Bosabalidis, JG Scandalios [1983] Proc NatI Acad Sci USA 80: 4455-4459). A mutation in barley reducing levels of peroxisomal catalase (AC Kendall et al. [1983] Planta 159: 505-511) does not reduce the amount of the isozyme with enhanced peroxidatic activity. Similarly, this isozyme is unaffected in dark-grown barley in spite of a 75% decrease in total catalase activity. These results suggest that this catalase isozyme is under separate genetic control in barley. This may also be the case in tobacco where the catalase isozyme with enhanced peroxidatic activity is an immunologically distinct protein (EA Havir, NA McHale [1989] Plant Physiol 89: 952-957).

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Section: Discussionmentioning

confidence: 99%

Enhanced-Peroxidatic Activity in Specific Catalase Isozymes of Tobacco, Barley, and Maize

Havir

McHale²

1989

Plant Physiol.

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“…Consistent with this possibility, both maize Catl and Cat2 sequences contain the conserved Ser-Arg-Leu and are localized to peroxisomes (Scandalios, 1990), but maize Cat3, in which the Ser is replaced by Thr, is mitochondria! (Scandalios et al, 1980). Efforts to correlate individual Arabidopsis catalase genes with catalase isozymes are ongoing.…”

Section: Dlscusslonmentioning

confidence: 99%

“…The maize Cat3 gene similarly exhibits maximal abundance in afternoon/ evening (Redinbaugh et al, 1990;Acevedo et al, 1991;Boldt and Scandalios, 1995). Maize Cat3 is mitochondrial (Scandalios et al, 1980), whereas Arabidopsis CAT3 has a consensus peroxisomal targeting signal and is expected to be localized to the peroxisome. Thus, a metabolic function for Arabidopsis CAT3 and for maize Cat3 remains speculative.…”

Section: Rlbsfcmentioning

confidence: 99%

Catalase Is Encoded by a Multigene Family in Arabidopsis thaliana (L.) Heynh

et al. 1996

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The catalase multigene family in Arabidopsis includes three genes encoding individual subunits that associate to form at least six isozymes that are readily resolved by nondenaturing gel electrophoresis. CATl and CAT3map to chromosome 1 , and CAT2maps to chromosome 4. The nucleotide sequences of the three coding regions are 70 to 72% identical. The amino acid sequences of the three catalase subunits are 75 to 84% identical and 87 to 94% similar, considering conservative substitutions. Both the individual isozymes and the individual subunit mRNAs show distinct patterns of spatial (organ-specific) expression. Six isozymes are detected in flowers and leaves and two are seen in roots. Similarly, mRNA abundance of the three genes varies among organs. All three mRNAs are highly expressed in bolts, and CAT2 and CAT3 are highly expressed in leaves.

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“…The presence of one form, CAT-3, in the mesophyll cells and possibly associated with mitochondria (23) suggests an additional role for catalase in leaf metabolism which is unrelated to photorespiration. It has been proposed that catalase in mitochondria would function to destroy H202 produced by the alternate oxidase (22,23).…”

Section: Introductionmentioning

confidence: 99%

“…In certain plant tissues such as cottonseed (15), tobacco (11), and corn (23,25), there is clear evidence that catalase exists in several forms which undergo specific changes during development. Such changes imply separate metabolic roles for the different forms of the enzyme but none have been unequivocally defined.…”

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confidence: 99%

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂

Havir¹,

McHale²

1989

Plant Physiol.

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The effect of high CO2 (1% C02/21% 02) on the activity of specific forms of catalase (CAT-1, -2, and -3) (EA Havir, NA McHale [1987] Plant Physiol 84: 450-455) in seedling leaves of tobacco (Nicotiana sylvestris, Nicotiana tabacum) was examined. In high C02, total catalase activity decreased by 50% in the first 2 days, followed by a more gradual decline in the next 4 days. The loss of total activity resulted primarily from a decrease in CAT-1 catalase. In contrast, the activity of CAT-3 catalase, a form with enhanced peroxidatic activity, increased 3-fold in high CO2 relative to aiontrols after 4 days. Short-term exposure to high CO2 indicated that the 50% loss of total activity occurs in the first 12 hours. Catalase levels increased to normal within 12 hours after seedlings were retumed to air. When seedlings were transferred to air after prolonged exposure to high CO2 (13 days), the levels of CAT-1 catalase were partially restored while CAT-3 remained at its elevated level. Levels of superoxide dismutase activity and those of several peroxisomal enzymes were not affected by high CO2. Total catalase levels did not decline when seedlings were exposed to atmospheres of 0.04% C02/5% 02 or 0.04% C02/1% 02, indicating that regulation of catalase in high CO2 is not related directly to suppression of photorespiration. Antibodies prepared against CAT-1 catalase from N. tabacum reacted strongly against CAT-1 catalase from both N. sylvestris and N. tabacum but not against CAT-3 catalase from either species. This observation, along with the rapid changes in CAT-I and the much slower changes in CAT-3 suggest that one form is not directly derived from the other. concentration (5, 19) have been shown to influence catalase activity in mature plants. For example, Fair et al. (5) demonstrated that catalase activity was suppressed when barley was grown in 1 to 5% CO2 and in 2% 02. Other peroxisomal enzymes were suppressed also and thus the effect in barley may be generally on peroxisomal turnover. In cases where catalase regulation is independent of peroxisomal turnover (e.g. Refs. 4, 6, and this paper) the mechanism of regulation has not been determined but could be the result of changes in rates of synthesis, degradation, or by covalent modification of the protein. The determination by Eising et al. (3) that the sharp decline in catalase activity in greening sunflower cotyledons was due to the formation of an inactive but largely intact protein molecule may be pertinent to studies of environmentally related changes in catalase activity.We previously showed that catalase activity in leaves of tobacco seedlings was distributed among several forms, one ofwhich had enhanced peroxidatic activity and that there was a change in distribution with time of development (l1). Furthermore, transfer of the seedlings to 1% C02/2 1% 02 for 4 d reduced total catalase activity by about 50% and altered the distribution ofthe activities. Here we show that the decline in catalase in high CO2 is very rapid and results primarily from the los...

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Cat3, a third gene locus coding for a tissue-specific catalase in maize: Genetics, intracellular location, and some biochemical properties

Cited by 91 publications

References 19 publications

Enhanced-Peroxidatic Activity in Specific Catalase Isozymes of Tobacco, Barley, and Maize

Enhanced-Peroxidatic Activity in Specific Catalase Isozymes of Tobacco, Barley, and Maize

Catalase Is Encoded by a Multigene Family in Arabidopsis thaliana (L.) Heynh

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂

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Cat3, a third gene locus coding for a tissue-specific catalase in maize: Genetics, intracellular location, and some biochemical properties

Cited by 91 publications

References 19 publications

Enhanced-Peroxidatic Activity in Specific Catalase Isozymes of Tobacco, Barley, and Maize

Enhanced-Peroxidatic Activity in Specific Catalase Isozymes of Tobacco, Barley, and Maize

Catalase Is Encoded by a Multigene Family in Arabidopsis thaliana (L.) Heynh

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO2

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Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂