Catalase activity and expression in developing sunflower seeds as related to drying

Bailly, Christophe; Leymarie, Juliette; Lehner, Arnaud; Rousseau, Sandra; Côme, D.; Corbineau, Françoise

doi:10.1093/jxb/erh050

Cited by 117 publications

(81 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results clearly showed that increased SOD activity appears to effectively mop up oxygen radicals during the early stage of maturation. Similarly, an increased SOD activity has been observed during seed maturation (Bailly et al, 2004). Analysis with native PAGE suggested there are five SOD isoforms visualized during seed maturation, but different isoform patterns were observed.…”

Section: Resultsmentioning

confidence: 79%

See 1 more Smart Citation

Changes of Antioxidant Enzyme and Phenylalanine Ammonia-Lyase Activities during Chimonanthus praecox Seed Maturation

Gao

2008

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Changes in peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and phenylalanine ammonia-lyase (PAL) activities were studied during Chimonanthus praecox seed maturation. According to our findings the protein content increased steadily from 8 to 12 weeks after flowering, and thereafter decreased significantly. Similarly, SOD and POD activities increased gradually up to 12 weeks after flowering and then declined. PAL activity declined gradually during seed maturation. CAT activity, however, showed no changes during seed maturation. By means of polyacrylamide gel electrophoresis (PAGE), SOD and POD isoenzymes were observed during seed maturation. The staining intensities of SOD and POD isoenzymes correlated well with SOD and POD activities as obtained by an assay in solution. These findings suggest that POD, SOD and PAL may be involved in the growth and development during Chimonanthus praecox seed maturation.

show abstract

Section: Resultsmentioning

confidence: 79%

“…The CAT activity has been shown to increase during seed development and is dependent on seed moisture content in sunflower (Bailly et al, 2004). However, no changes in the CAT activity were found during seed maturation, and the activity remained low, representing only 16 U/g fresh weight.…”

Section: Resultsmentioning

confidence: 97%

Changes of Antioxidant Enzyme and Phenylalanine Ammonia-Lyase Activities during Chimonanthus praecox Seed Maturation

Gao

2008

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

show abstract

“…Catalase and peroxidase converts hydrogen peroxide into compounds that are non-toxic to cells. The efficiency of the enzymatic, detoxification activity of the reactive species, especially of catalase, increases during seed development and is closely linked to the moisture content in sunflower seeds (BAILLY et al, 2004). In contrast, the activities of peroxidase and glutathione reductase decreased during desiccation of Triticum durum seeds (DE GARA et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

<b>‘Desiccation tolerance of <i>Rhamnidium elaeocarpum</i> Reissek (Rhamnaceae) seeds

et al. 2015

View full text Add to dashboard Cite

ABSTRACT. This study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the electrophoretic analysis of heat-resistant proteins were performed. A higher germination speed and respiratory rate was obtained for seeds with a water content of 12% w.b., and the activity of catalase isoforms was more pronounced in seeds with a higher degree of dehydration, which was in contrast to peroxidase, showed decreased activity. The seeds contained heatresistant proteins of low molecular weight that ranged from 48.7 to 13.2 kDa. We found that the acquisition of drying tolerance in R. Elaeocarpum Reissek seeds is associated more with catalase than with esterase and peroxidase.Keywords: drying, germination, isoenzymes, respiration rate, heat-resistant proteins.Tolerância à dessecação em sementes de Rhamnidium elaeocarpum Reissek (Rhamnaceae) RESUMO. O trabalho teve como objetivo investigar a tolerância a dessecação em sementes de Rhamnidium elaeocarpum Reissek por meio de alterações fisiológicas e bioquímicas. Os frutos foram coletados no município de Jaupaci, Estado de Goiás, quando as sementes apresentavam teor de água de 37% base úmida (b.u.), sendo em seguida mantidas em estufa de secagem com circulação de ar à temperatura de 35ºC até alcançarem os teores de água de 20 e 12% (b.u.). Foram avaliados os seguintes parâmetros: germinação; índice de velocidade de germinação; emergência; índice de velocidade de emergência; condutividade elétrica; taxa respiratória; perfil eletroforético das isoenzimas: catalase, peroxidase e esterase; atividade enzimática de endo-β-mananase e α-amilase e análise eletroforética de proteínas resistentes ao calor. Maior velocidade de germinação e taxa respiratória foi obtida para sementes com teor de água de 12% b.u. A atividade das isoformas de catalase foi mais pronunciada em sementes com maior grau de desidratação em relação à peroxidase que diminuiu sua atividade. As sementes apresentaram proteínas de baixo peso molecular resistentes ao calor, variando de 48,7 a 13,2 kDa. A aquisição de tolerância à secagem em sementes de R. elaeocarpum Reissek é mais associada à enzima catalase do que às enzimas esterase e peroxidase.Palavras-chave: secagem, germinação, isoenzimas, taxa respiratória, proteínas resistentes ao calor.

show abstract

“…In addition many environmental factors such as light (Feierabend et al, 1992) temperature (Omran, 1980;Nir et al, 1986), O 2 and CO 2 concentration (Fair et al, 1973;Monk et al, 1987) have been shown to influence catalase activity in mature plants. In fact this high level of CAT activity induced by grains drying was associated with a decrease in hydrogen peroxide level and in lipid peroxidation studied by Bailly et al (2004).…”

Section: Discussionmentioning

confidence: 87%

“…In our study highest activity of CAT was observed in grains which are in agreement with previous finding on Nigella sativa in which Kaleem et al (2006) observed high activity in grains. An increased CAT activity in sunflower (Helianthus annuus) grains was associated with a decrease in hydrogen peroxide level and in lipid peroxidation (Bailly et al, 2004). Activity of CAT is altered due to metabolic changes during various growth stages.…”

Section: Discussionmentioning

confidence: 99%

Comparative Antioxidant Potential and Bioactivity of Maize (Zea mays) Ear Tissues from Different Genotypes

Shahzadi¹,

Sheikh²,

Hameed³

et al. 2015

IJAB

View full text Add to dashboard Cite

The aim of this study was to determine the effects of maize (Zea mays L.) in diabetic patients in vitro. Three varieties (Golden, Sultan and Agatti: 85) were used to study the level of antioxidants and bio-molecules. Results showed that maize ears parts of all three varieties were more effective inhibitor in the oxidation process. Variety Golden silks was found to contain abundant activity of protease (22488 units/g f.wt.), α-amylase (390 units /g f.wt.), total sugar contents (307 mg/g f.wt.), and nonreducing sugar (288 mg/g f.wt.), and husks were rich in reducing sugar contents (16.9 mg/g f.wt.), and grains (kernels) in (15 mg/g f.wt.) protein content. Variety Sultan silks contained total phenolic content (38100 µM/g f.wt.), husk contained peroxidase activity (88655 U/g f.wt.), and grains manifested catalase (555 U/g f.wt.) activity. In varietyAgatti85 a highest activity of superoxide dismutase (292 U/g f.wt.) and malondialdehyde content (35 µM/g f.wt.) was recorded in silks. The findings of this research showed that maize probably inhibits the reaction of oxidation and decreases the complications occurring in diabetes mellitus. It was concluded that performed active parameters (total phenolics, SOD, peroxidase, catalase) may be responsible for higher antioxidant activity, which can be successfully used to reduce the extent of diabetes in the patients.

show abstract

Catalase activity and expression in developing sunflower seeds as related to drying

Cited by 117 publications

References 37 publications

Changes of Antioxidant Enzyme and Phenylalanine Ammonia-Lyase Activities during Chimonanthus praecox Seed Maturation

Changes of Antioxidant Enzyme and Phenylalanine Ammonia-Lyase Activities during Chimonanthus praecox Seed Maturation

<b>‘Desiccation tolerance of <i>Rhamnidium elaeocarpum</i> Reissek (Rhamnaceae) seeds

Comparative Antioxidant Potential and Bioactivity of Maize (Zea mays) Ear Tissues from Different Genotypes

Contact Info

Product

Resources

About