Catalytic autoantibodies against myelin basic protein (MBP) isolated from serum of autistic children impair in vitro models of synaptic plasticity in rat hippocampus

Gonzalez-Gronow, Mario; Cuchacovich, Miguel; Francos, Rina; Cuchacovich, Stephanie; Blanco, Angel; Sandoval, Rodrigo; Gomez, Cristian Farias; Valenzuela, Javier A.; Ray, Rupa; Pizzo, Salvatore V.

doi:10.1016/j.jneuroim.2015.07.006

Cited by 26 publications

(26 citation statements)

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“…Currently, pathological processes can be investigated using a variety of quantitative methods. Demyelination is analyzed histologically using Luxol fast blue (LFB) myelin staining (Sheng‐Kwei Song et al, ), and molecularly by measuring levels of myelin basic protein (MBP), a component of myelin (Gonzalez‐Gronowa et al, ). Axonal damage is quantified by measuring axonal swelling by immunostaining for β‐amyloid precursor protein (β‐APP; Sheng‐Kwei Song et al, ) and neurofilament protein 68 kDa (NF68; Lapin et al, ; Posmantur, Newcomb, Kampfl, & Hayes, ).…”

Section: Introductionmentioning

confidence: 99%

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Injury factors and pathological features of toxic milk mice during different disease stages

Zhou

Chen

et al. 2019

Brain and Behavior

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ObjectiveTo evaluate different injury factors and pathological characteristics of the brain at different disease stages in toxic milk (TX) mice, an animal model of Wilson's disease (WD).MethodsThirty TX mice (10 each at 3, 6 and 12 months old) and 30 age‐matched C57 mice were used in this study. Corrected phase (CP) values were determined from susceptibility‐weighted images. Myelin content was determined by measuring inhibition optical density values of Luxol fast blue‐stained sections. Neurofilament protein 68 kDa (NF68), β‐amyloid precursor protein (β‐APP), and myelin basic protein (MBP) levels, as well as copper and iron content, in brain nuclei of the TX mouse were evaluated. Gene amplification ratios for catalase (CAT), GSH peroxidase (GSH‐PX), nitric oxide synthase (NOS), and superoxide dismutase (SOD) in mouse brain were also determined.ResultsCompared with C57 mice, neuronal cell counts were decreased in 12‐months‐old TX mice (p = .011). Myelin content was decreased in the lenticular nucleus (p = .029), thalamus (p = .030), and brainstem (p = .034) of 6‐months‐old TX mice; decreases in the corresponding nuclei (p = .044, .037, and .032, respectively) were also found in 12‐months‐old TX mice. MBP values were lower in the lenticular nucleus and thalamus (p = .027 and .016, respectively) of 6‐months‐old TX mice and in the corresponding nuclei (p = .24 and .040) of 12‐months‐old TX mice. NF‐68 values were lower in the lenticular nucleus and thalamus (p = .034 and .037, respectively) of 6‐months‐old TX mice and in the corresponding nuclei (p = .006 and .012) of 12‐months‐old TX mice. β‐APP values were higher in the thalamus of 6‐months‐old (p = .037) and 12‐months‐old (p = .012) TX mice. Iron content was higher in the lenticular nucleus, thalamus, and cerebellum (p = .044, .038, and .029, respectively) of 6‐months‐old TX mice and in the corresponding nuclei (p = .017, .024, and .029) of 12‐months‐old TX mice. The NOS gene amplification multiple was higher (p = .039), whereas the SOD1 gene amplification multiple was lower (p = .041) in 12‐months‐old TX mice. There was no correlation between metal content or oxidation index and pathological index.ConclusionsThe pathological characteristics of the brains of TX mice may differ at different ages. Different pathogenic factors, including copper and iron deposition and abnormal oxidative stress, are present at different stages.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Injury factors and pathological features of toxic milk mice during different disease stages

Zhou

Chen

et al. 2019

Brain and Behavior

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“…They further propose specifically that myelin basic protein (MBP) and other proteins constituting the myelin sheath are attacked by the immune system in both autism and MS. This has been recognized by many others in autism [14,15] and MS [16][17][18][19][20]. In 1982, Weizman et al reported a cell-mediated autoimmune response to human MBP in 76% of the autistic children studied [16].…”

Section: Introductionmentioning

confidence: 72%

“…Gonzalez-Granow et al found high titres of autoantibodies in both the IgG and IgA classes specific to MBP in the serum of patients with autism [15]. The IgA antibodies in particular were shown to act as serine proteinases to degrade MBP in vitro.…”

Section: Rôle Of Acinetobacter and Pseudomonas Aeruginosa In Msmentioning

confidence: 99%

Glyphosate pathways to modern diseases VI: Prions, amyloidoses and autoimmune neurological diseases

Samsel¹,

Seneff²

2017

JBPC

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“…Thus, this natural clearance of immune complexes can lower the deleterious secondary effects such as inflammation and infiltration of various molecules of the immune system. Catalytic antibodies are described both in physiology as well as under various pathological conditions, such as asthma, hemophilia A, multiple sclerosis, rheumatoid arthritis, Hashimoto's thyroiditis, sepsis, graft versus host disease in transplantation, and others (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Depending on the target antigen, both pathogenic and beneficial role of catalytic antibodies have been described (10,19).…”

Section: Introductionmentioning

confidence: 99%

Catalytic antibodies in patients with systemic lupus erythematosus

Pradhan¹,

Pandit²,

Surve³

et al. 2018

Eur J Rheumatol

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Objective: Antibodies with catalytic (hydrolytic) properties to DNA or RNA have been reported in systemic lupus erythematosus (SLE). However, it is well known that ethnicity plays an important role in the presentation of SLE and severity of the disease; hence, these data may not truly represent a general feature of all SLE patients. Therefore, we have analyzed the hydrolyzing activity of immunoglobulin G (IgG) of SLE patients from the Indian population with an aim to decode whether the catalytic antibody response represents part of an active disease process. Methods: IgGs were isolated from the sera of 72 consecutive patients diagnosed with SLE. As a control, IgGs from healthy donors were used. The catalytic activity of IgG was measured by PFR-MCA and affinity-linked oligonucleotide nuclease assay. Results: IgGs from patients with SLE from the Indian subcontinent displayed significantly higher hydrolysis rates of both the surrogate substrate, PFR-MCA, and the DNA than IgG from healthy individuals. Intergroup comparisons of the IgG-PFR-MCA interactions with clinical manifestations of the disease demonstrated a significantly increased level of hydrolysis among the patients with renal involvement who tested positive for anti-dsDNA antibodies. The PFR-MCA hydrolysis also appears to be associated with the active disease (p=0.0988, vs. inactive group). Conclusion: The prevalence of catalytic antibodies represents a general feature of SLE patients, irrespective of their origin.

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Catalytic autoantibodies against myelin basic protein (MBP) isolated from serum of autistic children impair in vitro models of synaptic plasticity in rat hippocampus

Cited by 26 publications

References 46 publications

Injury factors and pathological features of toxic milk mice during different disease stages

Injury factors and pathological features of toxic milk mice during different disease stages

Glyphosate pathways to modern diseases VI: Prions, amyloidoses and autoimmune neurological diseases

Catalytic antibodies in patients with systemic lupus erythematosus

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