Catalytic Modification of Porous Two-Dimensional Ni-MOFs on Portable Electrochemical Paper-Based Sensors for Glucose and Hydrogen Peroxide Detection

Abstract

Rapid and accurate detection of changes in glucose (Glu) and hydrogen peroxide (H2O2) concentrations is essential for the predictive diagnosis of diseases. Electrochemical biosensors exhibiting high sensitivity, reliable selectivity, and rapid response provide an advantageous and promising solution. A porous two-dimensional conductive metal–organic framework (cMOF), Ni-HHTP (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene), was prepared by using a one-pot method. Subsequently, it was employed to construct enzyme-free paper-based electrochemical sensors by applying mass-producing screen-printing and inkjet-printing techniques. These sensors effectively determined Glu and H2O2 concentrations, achieving low limits of detection of 1.30 μM and 2.13 μM, and high sensitivities of 5573.21 μA μM−1 cm−2 and 179.85 μA μM−1 cm−2, respectively. More importantly, the Ni-HHTP-based electrochemical sensors showed an ability to analyze real biological samples by successfully distinguishing human serum from artificial sweat samples. This work provides a new perspective for the use of cMOFs in the field of enzyme-free electrochemical sensing, highlighting their potential for future applications in the design and development of new multifunctional and high-performance flexible electronic sensors.

A Study on the Mechanism and Properties of a Self-Powered H2O2 Electrochemical Sensor Based on a Fuel Cell Configuration with FePc and Graphene Cathode Catalyst Materials

“…Hydrogen peroxide (H 2 O 2 ) is not only widely used in the medical, health, industrial, and domestic fields, but it also plays an important role in the regulation of biological activities [ 1 , 2 , 3 , 4 ]. Moreover, H 2 O 2 is a product of oxidoreductase-catalyzed transformations; its quantification is used for the detection of other biologically important markers, e.g., glucose, lactate, uric acid, and glutamate [ 5 , 6 , 7 ]. Therefore, the detection of H 2 O 2 has been widely studied with different methods, such as fluorescence, spectrophotometry, and electrochemistry [ 8 , 9 , 10 ].…”

ZnO nanorods anchored on CNTs incorporating carbon cloth flexible electrode as a highly sensitive electrochemical sensor

“…The obtained results are listed in table 2. The normal window of glucose, UA and H 2 O 2 in healthy human serum is 3-8 mM [38], 120-460 μM [39], and 23.9-45.6 μM [40], respectively. What is more, the different concentrations of UA, glucose and H 2 O 2 in the sample were calculated according to the calibration curve, respectively.…”

An integrated leather-based fluid transport wearable sweat device for electrolyte balance monitoring

“…26–28 To obtain comprehensive physiological information, various sweat sensors have been systematically integrated with other electrophysiological sensor systems. 29–31 Therefore, the development of a multiplex flexible sweat sensor capable of accurately evaluating electrolyte balance is crucial for preventing water-salt imbalance-related disorders.…”