Catching bird flu in a droplet

Pipper, Juergen; Inoue, Masafumi; Ng, Lisa F. P.; Neužil, Pavel; Zhang, Yi; Novák, Lukáš

doi:10.1038/nm1634

Cited by 200 publications

(212 citation statements)

References 26 publications

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“…PCR may soon become more suitable for point-of-care use in resource-poor countries with ongoing advances in the development of hand-held, battery-operated devices using microfluidic methods for all-in-one DNA extraction, amplification, and detection (9,17,29,30). Unlike the situation with blood filtration, our PCR assay results are currently not reported quantitatively.…”

Section: Discussionmentioning

confidence: 99%

Toward Molecular Parasitologic Diagnosis: Enhanced Diagnostic Sensitivity for Filarial Infections in Mobile Populations

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Toward Molecular Parasitologic Diagnosis: Enhanced Diagnostic Sensitivity for Filarial Infections in Mobile Populations

et al. 2011

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“…26 In contrast, when thermally insulating substrates like glass are used, large temperature gradients can be generated. One special benefit of silicon is the ability to fabricate thin membranes, which reduce thermal mass and enable high temperature ramp-rates.…”

Section: The Remaining Advantages Of Silicon and Glassmentioning

confidence: 99%

A practical guide for the fabrication of microfluidic devices using glass and silicon

Iliescu¹,

Taylor²,

Avram³

et al. 2012

Biomicrofluidics

310

207

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This paper describes the main protocols that are used for fabricating microfluidic devices from glass and silicon. Methods for micropatterning glass and silicon are surveyed, and their limitations are discussed. Bonding methods that can be used for joining these materials are summarized and key process parameters are indicated. The paper also outlines techniques for forming electrical connections between microfluidic devices and external circuits. A framework is proposed for the synthesis of a complete glass/silicon device fabrication flow. I. WHY USE SILICON AND GLASS IN MICRO-AND NANO-FLUIDIC APPLICATIONS?Micro-and nano-fluidic technology continues to be embraced by biologists, 1-3 chemists, 4 and engineers throughout academia and industry. As its applications have expanded, there has been an explosion in the range of materials and processes used to fabricate these devices. The earliest microfluidic devices (e.g., gas 5 and liquid 6 chromatography devices) were made from silicon and glass and borrowed processes directly from semiconductor and microelectromechanical systems (MEMS) manufacturing. 7 Now, however, many researchers have moved away from silicon and glass, and instead use cast elastomers such as polydimethylsiloxane (PDMS)-which is ideal for swift prototyping-or thermoplastic polymers, which can be hot-embossed or injection-molded and are well suited to inexpensive manufacturing. Yet, there remain many applications where glass and silicon offer advantages over polymeric materials. In this paper, we highlight these advantages and offer a framework for selecting fabrication processes when using silicon and glass. A. The dominance of soft lithographyOver the last decade, PDMS has become virtually the default material for forming microfluidic devices, 8 because of the sheer ease with which it can be cast on to a micro-scale mould and then strongly bonded to glass. 9 The elastomeric nature of the material has been exploited to integrate fluidic valves and pumps on-chip and has simplified the production of multi-layer devices because the soft layers readily conform to each other. 10,11 Yet, the low stiffness (usually <1 MPa) of PDMS relative to amorphous thermoplastics, silicon, and glass has its own a) Authors to whom correspondence should be addressed. Electronic addresses: ciliescu@ibn.a-star.edu.sg and hkt@ntu.edu.sg. 6, 016505 (2012) drawbacks. High aspect-ratio channels are notoriously difficult to fabricate in PDMS because of their propensity to collapse. 12 Moreover, difficulties in automating the handling of such a soft material have hampered efforts to scale up PDMS device manufacturing. Meanwhile, PDMS's high oxygen and water permeabilities have proved both a blessing and a curse in different applications.The hydrophobic nature of PDMS can be an important consideration for some biological applications. In drug screening applications, for example, hydrophobic drugs as well as metabolites (urea or albumin) can be absorbed into the device's material due to hydrophobic--hydrophobic interaction...

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“…Pipper et al [54] used magnetic force to manipulate a free droplet containing superparamagnetic particles to detect the highly pathogenic avian influenza virus H5N1 in a throat swab sample. The RT-PCR results showed equally sensitive, 440% faster and 2,000-5,000% cheaper compared to commercially available tests.…”

Section: Droplet Pcr Chipmentioning

confidence: 99%

Microfluidic PCR Chips

Chen¹,

Chen²,

Yuan³

et al. 2011

Nano BioMed ENG

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The microfluidic polymerase chain reaction (PCR) chips have undergone extensive development and nowadays have become an important domain of miniaturization technology application. Here, we review the advances of microfluidic PCR chips over the past years, from the first single chamber stationary PCR chip to the new SlipChip PCR. First, the three distinct types of microfluidic PCR chips are discussed, including chamber stationary PCR chips, flow-through PCR chips and convection PCR chips. Then we focus on droplet PCR chips and SlipChip PCR. Although they are at an early stage, they show the great potential for high-throughput PCR and robust chip. Finally, general discussions on integrated chips are given. The low cost, portable, high-throughout integrated PCR chips will certainly be further developed in spite of many challenges.

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Catching bird flu in a droplet

Cited by 200 publications

References 26 publications

Toward Molecular Parasitologic Diagnosis: Enhanced Diagnostic Sensitivity for Filarial Infections in Mobile Populations

Toward Molecular Parasitologic Diagnosis: Enhanced Diagnostic Sensitivity for Filarial Infections in Mobile Populations

A practical guide for the fabrication of microfluidic devices using glass and silicon

Microfluidic PCR Chips

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