Catchment scale analysis of occurrence of antibiotic resistance genes in treated wastewater

Czatzkowska, Małgorzata; Harnisz, Monika; Kiedrzyńska, Edyta; Kiedrzyński, Marcin; Koniuszewska, Izabela; Korzeniewska, Ewa; Szklarek, Sebastian; Zalewski, Maciej

doi:10.1016/j.ecohyd.2019.06.002

Cited by 13 publications

(4 citation statements)

References 46 publications

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“…For example, our data revealed high counts of ARGs during the summer (Figure ), especially in one of the tertiary treated municipal wastewater sites (MA06) in the Mid-Atlantic region (Figure ). These findings are consistent with observations made by Garner et al and Czatzkowska et al when wastewaters were tested for multiple ARGs. , …”

Section: Discussionsupporting

confidence: 93%

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Variations in Bacterial Communities and Antibiotic Resistance Genes Across Diverse Recycled and Surface Water Irrigation Sources in the Mid-Atlantic and Southwest United States: A CONSERVE Two-Year Field Study

Malayil

Ramachandran

Chattopadhyay

et al. 2022

Environ. Sci. Technol.

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Reduced availability of agricultural water has spurred increased interest in using recycled irrigation water for U.S. food crop production. However, there are significant knowledge gaps concerning the microbiological quality of these water sources. To address these gaps, we used 16S rRNA gene and metagenomic sequencing to characterize taxonomic and functional variations (e.g., antimicrobial resistance) in bacterial communities across diverse recycled and surface water irrigation sources. We collected 1 L water samples (n = 410) between 2016 and 2018 from the Mid-Atlantic (12 sites) and Southwest (10 sites) U.S. Samples were filtered, and DNA was extracted. The V3–V4 regions of the 16S rRNA gene were then PCR amplified and sequenced. Metagenomic sequencing was also performed to characterize antibiotic, metal, and biocide resistance genes. Bacterial alpha and beta diversities were significantly different (p < 0.001) across water types and seasons. Pathogenic bacteria, such as Salmonella enterica, Staphylococcus aureus, and Aeromonas hydrophilia were observed across sample types. The most common antibiotic resistance genes identified coded against macrolides/lincosamides/streptogramins, aminoglycosides, rifampin and elfamycins, and their read counts fluctuated across seasons. We also observed multi-metal and multi-biocide resistance across all water types. To our knowledge, this is the most comprehensive longitudinal study to date of U.S. recycled water and surface water used for irrigation. Our findings improve understanding of the potential differences in the risk of exposure to bacterial pathogens and antibiotic resistance genes originating from diverse irrigation water sources across seasons and U.S. regions.

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Section: Discussionsupporting

confidence: 93%

“…These findings are consistent with observations made by Garner et al and Czatzkowska et al when wastewaters were tested for multiple ARGs. 84 , 85 …”

Section: Discussionmentioning

confidence: 99%

Variations in Bacterial Communities and Antibiotic Resistance Genes Across Diverse Recycled and Surface Water Irrigation Sources in the Mid-Atlantic and Southwest United States: A CONSERVE Two-Year Field Study

Malayil

Ramachandran

Chattopadhyay

et al. 2022

Environ. Sci. Technol.

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“…The authors indicated also that in order to determine the effect of microbiological contamination on other components of river ecosystem, there is a need for further ecohydrological research within the watercourse. Another study confirmed the presence of nearly all the studied ARGs in water samples from the Pilica river [46]. Interestingly, a higher prevalence of ARGs was noted in spring than in summer, and in treated wastewater effluent from small WWTPs, indicating that those WWTPs are not effective in ARG removal.…”

Section: Genomics In Monitoring Of Cecs Threats To Freshwater Ecosystems and Analysis Of Cause-effect Relationships-assessment Approachmentioning

confidence: 80%

“…biomonitoring and ecotoxicological assessment of the selected compounds on C. carassius comet assay [41] ARGs determination of ARGs occurrence along the studied section of Pilica river PCR [45] ARGs determination of ARGs occurrence in wastewater effluent PCR [46] ARGs determination of ARGs occurrence in a microcosm of constructed wetlands PCR [57] antibiotics (sulfonamides, β-lactam antibiotics, tetracycline)…”

Section: Levelmentioning

confidence: 99%

Molecular Methods as Potential Tools in Ecohydrological Studies on Emerging Contaminants in Freshwater Ecosystems

Mierzejewska

Urbaniak

2020

Water

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Contaminants of emerging concern (CECs) present a threat to the functioning of freshwater ecosystems. Their spread in the environment can affect both plant and animal health. Ecohydrology serves as a solution for assessment approaches (i.e., threat identification, ecotoxicological assessment, and cause–effect relationship analysis) and solution approaches (i.e., the elaboration of nature-based solutions: NBSs), mitigating the toxic effect of CECs. However, the wide array of potential molecular analyses are not fully exploited in ecohydrological research. Although the number of publications considering the application of molecular tools in freshwater studies has been steadily growing, no paper has reviewed the most prominent studies on the potential use of molecular technologies in ecohydrology. Therefore, the present article examines the role of molecular methods and novel omics technologies as essential tools in the ecohydrological approach to CECs management in freshwater ecosystems. It considers DNA, RNA and protein-level analyses intended to provide an overall view on the response of organisms to stress factors. This is compliant with the principles of ecohydrology, which emphasize the importance of multiple indicator measurements and correlation analysis in order to determine the effects of contaminants, their interaction with other environmental factors and their removal using NBS in freshwater ecosystems.

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Removal of antimicrobial resistance determinants from wastewater: a risk perspective on conventional and emerging technologies

Moura

Picão

2022

Emerging Contaminants in the Environment

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Catchment scale analysis of occurrence of antibiotic resistance genes in treated wastewater

Cited by 13 publications

References 46 publications

Variations in Bacterial Communities and Antibiotic Resistance Genes Across Diverse Recycled and Surface Water Irrigation Sources in the Mid-Atlantic and Southwest United States: A CONSERVE Two-Year Field Study

Variations in Bacterial Communities and Antibiotic Resistance Genes Across Diverse Recycled and Surface Water Irrigation Sources in the Mid-Atlantic and Southwest United States: A CONSERVE Two-Year Field Study

Molecular Methods as Potential Tools in Ecohydrological Studies on Emerging Contaminants in Freshwater Ecosystems

Removal of antimicrobial resistance determinants from wastewater: a risk perspective on conventional and emerging technologies

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