Catfish Bacterial Diseases

Plumb, John A.; Hanson, Larry A.

doi:10.1002/9780470958353.ch11

Cited by 8 publications

(4 citation statements)

References 155 publications

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“…Flavobacterium columnare , the causative agent of columnaris disease, is a globally distributed pathogen of fresh and brackish water fish. 14 A member of family Flavobacteriaceae, F. columnare produces spreading, rhizoid, discrete yellow-to-orange colonies on agar plates following 48-h incubation at 25–30°C. 14 Columnaris disease was first described from an outbreak in fingerling buffalo fish ( Ictiobus sp.…”

Section: Introductionmentioning

confidence: 99%

“…14 A member of family Flavobacteriaceae, F. columnare produces spreading, rhizoid, discrete yellow-to-orange colonies on agar plates following 48-h incubation at 25–30°C. 14 Columnaris disease was first described from an outbreak in fingerling buffalo fish ( Ictiobus sp. ), with the most notable characteristic being the column-like masses of bacteria observed on wet mounts of affected tissue.…”

Section: Introductionmentioning

confidence: 99%

“…9,20 Isolation of F. columnare on agar plates can be complicated because diagnostic samples often contain numerous bacterial species that outcompete F. columnare . 14…”

Section: Introductionmentioning

confidence: 99%

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Validation of a quantitative PCR assay for the detection of 2 Flavobacterium columnare genomovars

et al. 2020

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Flavobacterium columnare is the causative agent of columnaris disease in a variety of fish hosts. Using modifications to previously established protocols, a quantitative PCR (qPCR) assay was validated for the detection of 2 predominant F. columnare genomovars. The oligonucleotide primer and probe combination was designed to amplify a 203-bp region of the chondroitin AC lyase gene (GenBank AY912281) of F. columnare. There were no significant differences in amplification between genomovars. Comparable quantities of genomic DNA from 10 F. columnare strains, 5 representatives of each genomovar, produced similar results. Serial dilutions of purified PCR product demonstrated the limit of sensitivity for the assay was ~ 10 copies per reaction. The presence of gill and spleen tissue did not significantly affect the sensitivity of the assay. Comparably, bacterial DNA detected from the liver and kidney was less sensitive than pure bacterial DNA. However, detection from these tissues was within one order of magnitude of other tissues, indicating this reduction may have minimal analytic significance. This validated assay was used to approximate the minimum infectious dose for F. columnare isolate 94-081 in channel catfish and assess bacterial loads in gill and kidney tissues 48 h post-infection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Validation of a quantitative PCR assay for the detection of 2 Flavobacterium columnare genomovars

et al. 2020

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“…The three bacterial species responsible for most of these losses are Edwardsiella ictaluri, Flavobacterium columnare , and Aeromonas hydrophila . These pathogens are the causative agents of enteric septicemia of catfish (ESC), columnaris disease, and motile Aeromonas septicemia (MAS), respectively (4, 5).…”

Section: Introductionmentioning

confidence: 99%

Recombinant ATPase of Virulent Aeromonas hydrophila Protects Channel Catfish Against Motile Aeromonas Septicemia

et al. 2019

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Channel catfish farming dominates the aquaculture industry in the United States. However, epidemic outbreaks of motile Aeromonas septicemia (MAS), caused by virulent Aeromonas hydrophila (vAh), have become a prominent problem in the catfish industry. Although vaccination is an effective preventive method, there is no vaccine available against MAS. Recombinant proteins could induce protective immunity. Thus, in this work, vAh ATPase protein was expressed, and its protective capability was evaluated in catfish. The purified recombinant ATPase protein was injected into catfish, followed by experimental infection with A. hydrophila strain ML09-119 after 21 days. Results showed catfish immunized with ATPase exhibited 89.16% relative percent survival after challenge with A. hydrophila strain ML09-119. Bacterial concentrations in liver, spleen, and anterior kidney were significantly lower in vaccinated fish compared with the non-vaccinated sham group at 48 h post-infection ( p < 0.05). Catfish immunized with ATPase showed a significant ( p < 0.05) higher antibody response compared to the non-vaccinated groups. Overall, ATPase recombinant protein has demonstrated potential to stimulate protective immunity in catfish against virulent A. hydrophila infection.

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Monoclonal antibody based Dot-ELISA and indirect fluorescence antibody technique for detecting Edwardsiella ictaluri infection in yellow catfish ( Pelteobagrus fulvidraco )

Huang

Zhang

et al. 2017

Aquaculture and Fisheries

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Catfish Bacterial Diseases

Cited by 8 publications

References 155 publications

Validation of a quantitative PCR assay for the detection of 2 Flavobacterium columnare genomovars

Validation of a quantitative PCR assay for the detection of 2 Flavobacterium columnare genomovars

Recombinant ATPase of Virulent Aeromonas hydrophila Protects Channel Catfish Against Motile Aeromonas Septicemia

Monoclonal antibody based Dot-ELISA and indirect fluorescence antibody technique for detecting Edwardsiella ictaluri infection in yellow catfish ( Pelteobagrus fulvidraco )

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