Cationic Bioactive Peptide from the Seeds of Benincasa hispida

Abstract

A designated bioactive peptide “Hispidalin” purified from the seeds of Benincasa hispida, which is a medicinal plant, belongs to Cucurbitaceae family. Purification was achieved by using a procedure consisting of extraction from potassium phosphate buffer followed by FPLC and HPLC steps. Based on amino acid residue, this peptide is amphipathic and basic with one net positive charge having isoelectric pH 8.1. This peptide is without sulphur containing amino acid suggesting its extended conformation lacking double bond secondary structure. The results obtained from MALDI-TOF suggested that Hispidalin is of molecular mass 5.7 KDa with 49 amino acid residues and confirmed SDS-PAGE resolved ∼6.0 KDa protein band. This novel and unknown peptide “Hispidalin” showed broad and potent inhibitory effects against various human bacterial and fungal pathogens; its growth inhibition was significantly comparable with commercial antibacterial and antifungal drugs. The Hispidalin at 40 μg/mL concentration exhibited 70.8% DPPH free radical-scavenging activity and 69.5% lipid peroxide inhibition. Thus, in the present study, Hispidalin demonstrated remarkable antimicrobial and antioxidant potentials from the seeds of B. hispida.

Successful treatment of antibiotic-associated diarrhea with a herbal medicine, daiobotampito

“…DBT consists of a mixture of benincasa seed, peach kernel, moutan bark, rhei rhizoma, and natrii sulfas. Of these, the antimicrobial activity or anti‐inflammatory potency of rhei rhizoma and benincasa seed have been reported.…”

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“…This peptide was 5.7 kDa (49 amino acid residues), basic and amphipathic 129 . It does not comprise of any sulphur containing amino acid, which suggested that it does not have any secondary structure.…”

Determinación del efecto cicatrizante de Piper aduncum (Matico) en fibroblastos humanos

“…Estos resultados respaldan la hipótesis de que péptidos activos y proteínas derivadas de plantas tienen efectos beneficiosos en la salud humana, en este caso, en el proceso de cicatrización (7,26) .…”

“…Además de estos componentes, las plantas son las principales fuentes de péptidos, los cuales son moléculas de proteínas de menos de 10 kDa; que pueden existir de forma natural o ser derivados de secuencias de proteínas nativas (5,6) . En los últimos años, una amplia evidencia científica ha previsto la existencia de péptidos activos biológicos y proteínas derivadas de plantas que podrían tener efectos beneficiosos sobre la salud humana (7) .…”