Caulobacter profunda sp. nov., isolated from deep freshwater sediment

Abstract

The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or nonmotile (stalked) and rod-shaped bacteria, DS48-5-2T and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken The genus Caulobacter is well known to have a different reproductive mode of division into non-motile forms with prosthecae (Staley, 1968) and motile ones with a polar flagellum, which helps to disperse the population at each generation, minimizing competition for resources (Abraham et al., 1999). Since the first isolation of a species of the genus Caulobacter was reported by Löeffler (1890), the genus has undergone reclassification by Abraham et al. (1999) and Chen et al. Henrici & Johnson, 1935). This study describes two bacterial strains, DS48-5-2 T and DS48-6-3, and establishes their taxonomic positions using a polyphasic approach that includes determining their phenotypic and chemotaxonomic properties and a detailed phylogenetic investigation based on their 16S rRNA gene sequences.Strains DS48-5-2T and DS48-6-3 were isolated from a sediment sample collected at 48 m deep taken from Daechung Reservoir in South Korea. Sediments were sampled using a KB core sampler (Wildco) on a boat. Approximately 100 mg of the sediment was first serially diluted in 0.85 % saline solution, and then aliquots of each serial dilution were spread on an R2A agar (Difco) and incubated at 25 uC. After 6 days, two yellow colonies, designated DS48-5-2T and DS48-6-3, were isolated. The

Characterization of bacterial community associated with phytoplankton bloom in a eutrophic lake in South Norway using 16S rRNA gene amplicon sequence analysis

“…aeruginosa [14, 18]. Another Alphaproteobacteria species was Caulobacter profundus [107]. Caulobacter are known to be associated with cyanobacterial blooms and enhance the growth of Microcystis [13, 108].…”

Linkage of microbial kinetics and bacterial community structure of MBR and hybrid MBBR–MBR systems to treat salinity‐amended urban wastewater

“…Several species could be able to develop denitrification by reducing nitrite, nitrate, or both, such as Rhodanobacter, Luteimonas, Thuera, Comamonas, and Caulobacter, reported for these metabolisms. 31,32,[44][45][46] Finally, among all the major represented phylotypes, Luteimonas and Thermomonas can biodegrade N-acetylglucosamine. 30,38 In this sense, the dynamics in the bacterial community structure showed a transition between bacteria from different genera but with the same ecological capabilities.…”

Caulobacter flavus sp. nov., a stalked bacterium isolated from rhizosphere soil

“…was isolated from Russian polar tundra soil by Berestovskaya et al (2006). At the time of writing, the genus Caulobacter comprises eight recognized species, which were isolated from a variety of environments, such as soil, water, rhizosphere soil, activated sludge system, deep freshwater sediment: Caulobacter segnis (Urakami et al , 1990; Abraham et al , 1999), Caulobacter vibrioides (Henrici & Johnson, 1935; Poindexter, 1964), Caulobacter henricii (Poindexter, 1964), Caulobacter fusiformis (Poindexter, 1964; Poindexter & Lewis, 1966), Caulobacter mirabilis (Abraham et al , 2008), Caulobacter ginsengisoli (Liu et al , 2010), Caulobacter profundus (Jin et al , 2014) and Caulobacter daechungensis (Jin et al , 2013). Cell division of the genus Caulobacter is mostly asymmetrical with two different cell morphologies: one with a stalk and another with a single polar flagellum, except C. segnis .…”