Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Kunert‐Keil, Christiane; Gredes, Tomasz; Lucke, Silke; Morgenstern, Sven; Mielczarek, Agnieszka; Sporniak‐Tutak, Katarzyna; Gedrange, Tomasz; Spassov, Alexander

doi:10.5603/fhc.2011.0041

Cited by 15 publications

(12 citation statements)

References 45 publications

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“…The above listed proteins are majorly involved in cellular regeneration and structural adaptations in dystrophinopathy. The increased oligomerisation of caveolin‐1 would agree with its elevated concentration in mdx muscle and increased numbers of vesicular invaginations of the sarcolemma, called caveolae, in dystrophic muscles . Caveolae structures are involved in signalling processes and endocytic trafficking, and changes in caveolin proteins appear to be related to regenerative processes .…”

Section: Discussionmentioning

confidence: 82%

Comparative gel‐based proteomic analysis of chemically crosslinked complexes in dystrophic skeletal muscle

et al. 2018

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Duchenne muscular dystrophy is a highly progressive muscle wasting disease with a complex pathophysiology that is based on primary abnormalities in the dystrophin gene. In order to study potential changes in the oligomerization of high‐molecular‐mass protein complexes in dystrophic skeletal muscle, chemical crosslinking was combined with mass spectrometric analysis. The biochemical stabilization of protein interactions was carried out with the homo‐bifunctional and amine‐reactive agent bis[sulfosuccinimidyl]suberate, followed by protein shift analysis in one‐dimensional gels. The proteomic approach identified 11 and 15 protein species in wild type versus dystrophic microsomal fractions, respectively, as well as eight common proteins, with an electrophoretic mobility shift to very high molecular mass following chemical crosslinking. In dystrophin‐deficient preparations, several protein species with an increased tendency of oligomerisation were identified as components of the sarcolemma and its associated intra‐ and extracellular structures, as well as mitochondria. This included the sarcolemmal proteins myoferlin and caveolin, the cytoskeletal components vimentin and tubulin, extracellular collagen alpha‐1(XII) and the mitochondrial trifunctional enzyme and oxoglutarate dehydrogenase. These changes are probably related to structural and metabolic adaptations, especially cellular repair processes, which agrees with the increased oligomerisation of myosin‐3, myosin‐9 and actin, and their role in cellular regeneration and structural adjustments in dystrophinopathy.

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Section: Discussionmentioning

confidence: 82%

Comparative gel‐based proteomic analysis of chemically crosslinked complexes in dystrophic skeletal muscle

et al. 2018

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“…However, in this study, male dystrophic patients were compared to much older control subjects of both sexes. In contrast to DMD patients, decreased level of VEGF was noticed in gastrocnemius (GM) [ 71 ] and soleus [ 85 ] muscles of mdx mice as well as in the diaphragm of mdx /utrn −/− animals [ 86 ]. Moreover, transcriptome-based analysis of GM performed by our group revealed markedly diminished level of VEGF in mdx mice in comparison to wild-type animals (unpublished data).…”

Section: The Role Of Vegf In Dmd—pro-angiogenic and Pro-myogenic Effementioning

confidence: 99%

Targeting angiogenesis in Duchenne muscular dystrophy

Podkalicka

Mucha

Dulak

et al. 2019

Cell. Mol. Life Sci.

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Duchenne muscular dystrophy (DMD) represents one of the most devastating types of muscular dystrophies which affect boys already at early childhood. Despite the fact that the primary cause of the disease, namely the lack of functional dystrophin is known already for more than 30 years, DMD still remains an incurable disease. Thus, an enormous effort has been made during recent years to reveal novel mechanisms that could provide therapeutic targets for DMD, especially because glucocorticoids treatment acts mostly symptomatic and exerts many side effects, whereas the effectiveness of genetic approaches aiming at the restoration of functional dystrophin is under the constant debate. Taking into account that dystrophin expression is not restricted to muscle cells, but is present also in, e.g., endothelial cells, alterations in angiogenesis process have been proposed to have a significant impact on DMD progression. Indeed, already before the discovery of dystrophin, several abnormalities in blood vessels structure and function have been revealed, suggesting that targeting angiogenesis could be beneficial in DMD. In this review, we will summarize current knowledge about the angiogenesis status both in animal models of DMD as well as in DMD patients, focusing on different organs as well as age-and sex-dependent effects. Moreover, we will critically discuss some approaches such as modulation of vascular endothelial growth factor or nitric oxide related pathways, to enhance angiogenesis and attenuate the dystrophic phenotype. Additionally, we will suggest the potential role of other mediators, such as heme oxygenase-1 or statins in those processes.

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“…; Kunert‐Keil et al. ). The criteria for a capillary‐like structure were central lumina surrounded by caveolin‐1 or CD34 positively stained cells.…”

Section: Methodsmentioning

confidence: 99%

“…Sections were counter stained in hematoxylin and then cover-slipped. As control for the specificity of the caveolin-1 antibody, antigen-pre-incubated antibodies were used as shown recently (Kunert-Keil et al 2011). For negative controls, the primary antibody was replaced by PBS and PBS used in the same way.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…For standardization of the measurement, the optical density of white background color was attuned to 250 in each picture. For all sections, mean optical densities and the quantity of capillary-like structures were assessed using KSRun software (imaging system KS400, release 3.0; Zeiss, Vision GmbH, Munich, Germany) (Gerger et al 2004;Kunert-Keil et al 2011).…”

Section: Quantitative and Qualitative Analysis Of Capillary-like Strumentioning

confidence: 99%

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Histological evaluation at different times after augmentation of extraction sites grafted with a magnesium‐enriched hydroxyapatite: double‐blinded randomized controlled trial

Canullo¹,

Heinemann

Gedrange

et al. 2012

Clinical Oral Implants Res

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Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Cited by 15 publications

References 45 publications

Comparative gel‐based proteomic analysis of chemically crosslinked complexes in dystrophic skeletal muscle

Comparative gel‐based proteomic analysis of chemically crosslinked complexes in dystrophic skeletal muscle

Targeting angiogenesis in Duchenne muscular dystrophy

Histological evaluation at different times after augmentation of extraction sites grafted with a magnesium‐enriched hydroxyapatite: double‐blinded randomized controlled trial

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