Caviibacter abscessus gen. nov., sp. nov., a member of the family Leptotrichiaceae isolated from guinea pigs (Cavia porcellus)

Eisenberg, Tobias; Glaeser, Stefanie P.; Ewers, Christa; Semmler, Torsten; Drescher, Birgit; Kämpfer, Peter

doi:10.1099/ijsem.0.000922

Cited by 21 publications

(22 citation statements)

References 41 publications

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“…The major fatty acids C 16 : 0 , C 18 : 0 , C 18 : 1 !9c and C 18 : 2 !6,9c/ 18 : 0 ANTE were in accordance with those found for other members of the same family before (Eisenberg et al, 2016a, 2016b, Pins et al, 1996, Rowbotham, 1983, Rygg & Bruun, 1992 Table 2.…”

supporting

confidence: 75%

Oceanivirga salmonicida gen. nov., sp. nov., a member of the Leptotrichiaceae isolated from Atlantic salmon (Salmo salar)

Eisenberg

Kämpfer

Ewers

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Oceanivirga salmonicida gen. nov., sp. nov., a member of the Leptotrichiaceae isolated from Atlantic salmon (Salmo salar) A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase-and catalase-negative, non-spore-forming, non-motile bacterium was originally isolated in 1992 from moribund, seawater farmed Atlantic salmon with multifocal tissue necrosis. Strain AVG 2115 T displayed considerable similarities with Streptobacillus moniliformis, one of the two etiological agents of rat bite fever, and has been stored as Streptobacillus sp. NCIMB 703044 T . On the basis of 16S rRNA gene sequence analyses, this strain displayed >99 % sequence similarities with uncultured bacterial clones from the digestive tracts of marine mammals, followed by Sneathia sanguinegens CCUG 41628 T (92.7 %), 'Sneathia amnii' Sn35 (92.5 %), Caviibacter abscessus CCUG 39713 T (92.2 %), Streptobacillus ratti OGS16 T (91.3 %), Streptobacillus notomytis AHL 370-1 T (91.2 %), S. moniliformis DSM 12112 T (91.0 %), Streptobacillus felis 131000547 T (90.9 %) and Streptobacillus hongkongensis DSM 26322 T (89.7 %). Sequence similarities to all other taxa were below 89 %. Phylogenetic analysis for strain NCIMB 703044 T revealed highly similar results for gyrB, groEL and recA nucleotide and deduced amino acid sequence analyses independent of the employed treeing method. Average nucleotide identities (ANI) for complete genomes ranged from 66.00 % to 72.08 % between strain NCIMB 703044 T and the type strains of Sebaldella termitidis, Leptotrichia buccalis, Streptobacillus moniliformis, Sneathia sanguinegens and Caviibacter abscessus. Chemotaxonomic and physiological data of strain NCIMB 703044 t were in congruence with closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis was capable to clearly discriminate strain NCIMB 703044 T from all currently described taxa of the family Leptotrichiaceae. On the basis of these data we propose the novel taxon Oceanivirga salmonicida gen. nov. sp. nov. with the type strain AVG 2115 T (=NCIMB 703044 T ) (=DSM 101867 T ). The G+C content is 25.4 %, genome size is 1.77 Mbp.Abbreviations: MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry; ML, maximum-likelihood; ANI, average nucleotide identity; POCP, percentage of conserved proteins; DDH, DNA-DNA hybridization.

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supporting

confidence: 75%

Oceanivirga salmonicida gen. nov., sp. nov., a member of the Leptotrichiaceae isolated from Atlantic salmon (Salmo salar)

Eisenberg

Kämpfer

Ewers

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…We have undertaken a first step into this direction by analysing a broad spatio-temporal collection of strains, thereby including especially species with regular evidence for pathologies. Firstly, the large dataset from this study has been utilized for the confirmation of our phylogenetic picture from earlier studies [18, 30, 37, 38]. An intra-genus phylogeny that was based on 775 orthologous genes revealed a very similar picture to previous studies involving only four selected functional genes (Figs.…”

Section: Discussionsupporting

confidence: 63%

“…d. a.Rat ( Rattus rattus )Oral swabJapan[39]this studySAMN0409967029OGS16 T Streptobacillus ratti n. d. a.Rat ( Rattus rattus )Oral swabJapan[39][18]SAMN0409967530CCUG 41628 T Sneathia sanguinegens 1999HumanBloodSweden[58, 59][38]LOQF0000000031Sn35 “Sneathia amnii” n. d. a.HumanVaginal microbiotan.…”

Section: Resultsmentioning

confidence: 99%

“…d. a.NZ_AWVL0000000044CCUG 39713 T Caviibacter abscessus 1998Guinea pigCervical abscessSwedenn. d. a.[38]LOQG00000000451510011837 Caviibacter abscessus 2015Guinea pigCervical abscessGermany[38][38]LOQH0000000046AVG2115 T Oceanivirga salmonicida 1992Atlantic salmonSepticaemiaIreland[32, 61][37]LOQI0000000047ATCC 25586 Fusobacterium nucleatum subsp. nucleatum n.…”

Section: Resultsmentioning

confidence: 99%

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Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis

et al. 2016

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BackgroundThe Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties.ResultsOur data provide a novel and reliable basis of support for previously established phylogeny from this group and give a deeper insight into characteristics of genome structure and gene functions. Full genome analyses revealed that most S. moniliformis strains under study form a heterogeneous population without any significant clustering. Analysis of infra-species variability for this highly pathogenic rat bite fever organism led to the detection of three specific variable number tandem analysis loci with high discriminatory power.ConclusionsThis highly useful and economical tool can be directly employed in clinical samples without laborious prior cultivation. Our and prospective case-specific data can now easily be compared by using a newly established MLVA database in order to gain a better insight into the epidemiology of this presumably under-reported zoonosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3206-0) contains supplementary material, which is available to authorized users.

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“…26 Unfortunately, this test failed to unequivocally detect true infection due to lack in specificity and therefore immune blotting (IB) with whole cell antigens was advised to confirm positive or doubtful reactions from ELISA. 25 Interestingly, antibodies in sera of guinea pigs were also detected by ELISA, but guinea pigs were resistant to oral or nasal infection with a rat strain of S. moniliformis 131 and strains infecting guinea pigs formerly assigned to S. moniliformis were found to represent a novel species, 69 88 Use of membrane proteins of S. moniliformis instead of whole bacterial cells reduces the background reactions in ELISA and increases its specificity (Nicklas, unpublished data). Proteins can easily be prepared as described by Livingston et al 132 for Helicobacter hepaticus.…”

Section: Indirect Techniques For Detection Of Infectionmentioning

confidence: 99%

Approved and novel strategies in diagnostics of rat bite fever and otherStreptobacillusinfections in humans and animals

Eisenberg¹,

Ewers

Rau

et al. 2016

Virulence

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Rat bite fever (RBF), a worldwide occurring and most likely under-diagnosed zoonosis caused by Streptobacillus moniliformis, represents the most prominent disease of Streptobacillus infections. Recently, novel members have been described, from which a reservoir in rats and other animal species and a zoonotic potential can be assumed. Despite regularly published case reports, diagnostics of RBF continues to represent a 'diagnostic dilemma', because the mostly applied 16S rRNA sequence analysis may be uncertain for proper pathogen identification. Virtually nothing is known regarding prevalence in humans and animal reservoirs. For a realistic assessment of the pathogen's spread, epidemiology and virulence traits, future studies should focus on the genomic background of Streptobacillus. Full genome sequence analyses of a representative collection of strains might facilitate to unequivocally identify and type isolates. Prevalence studies using selective enrichment mechanisms may also enable the isolation of novel strains and candidate species of this neglected group of microorganisms.

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Caviibacter abscessus gen. nov., sp. nov., a member of the family Leptotrichiaceae isolated from guinea pigs (Cavia porcellus)

Cited by 21 publications

References 41 publications

Oceanivirga salmonicida gen. nov., sp. nov., a member of the Leptotrichiaceae isolated from Atlantic salmon (Salmo salar)

Oceanivirga salmonicida gen. nov., sp. nov., a member of the Leptotrichiaceae isolated from Atlantic salmon (Salmo salar)

Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis

Approved and novel strategies in diagnostics of rat bite fever and otherStreptobacillusinfections in humans and animals

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