Cb-15 * Nkcc1 Regulates Migration Capacity of Glioblastoma Tumor Initiating Cells by Modulating Actin Cytoskeleton Through Small Rho Gtpases

Schiapparelli, Paula; Magaña-Maldonado, Roxana; Hamilla, Susan M.; Sibener, Leslie; Martínez-Gutiérrez, Juan Carlos; Aranda‐Espinoza, Helim; Quiñones‐Hinojosa, Alfredo

doi:10.1093/neuonc/nou241.14

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“…NKCC1 modulates migration of GBM cells by at least two different mechanisms: cell volume regulation (through ion transport) and regulation of the actin cytoskeleton. 23 Chloride channels in glioma cells have been associated with tumor invasiveness, presumably by facilitating cell shape and cell volume changes that are more conducive to migration and invasion. Blockade of glioma Cl − channels specifically inhibits tumor cell migration in a dose-dependent manner.…”

Section: Cytoskeleton and Structural Organizationmentioning

confidence: 99%

Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy

Dučić

Paunesku

Chen

et al. 2017

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The glioblastoma (GBM) is characterized by a short median survival and an almost 100% tumor related mortality. GBM cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores application of X-ray and visible light microscopy to display the elemental and structural images of cells from 3 patient derived GMB samples and an established GMB cell line. Slight differences in elemental concentrations, in actin cytoskeleton organization and cell morphology were noted between all cells types by X-ray fluorescence and full field soft X-ray microscopy, as well as the Structured Illumination Super-resolution Microscope (SIM). Different sample preparation approaches were used to match each imaging technique. While preparation for SIM included cell fixation and staining, intact frozen hydrated cells were used for the trace element imaging by hard X-ray fluorescence and exploration of the structural features by soft X-ray absorption tomography. Each technique documented differences between samples with regard to morphology and elemental composition and underscored the importance of use of multiple patient derived samples for detailed GBM study.

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Section: Cytoskeleton and Structural Organizationmentioning

confidence: 99%