CCDC106 promotes non-small cell lung cancer cell proliferation

Abstract

Coiled-coil domain containing (CCDC) family members enhance tumor cell proliferation, and high CCDC protein levels correlate with unfavorable prognoses. Limited research demonstrated that CCDC106 may promote the degradation of p53/TP53 protein and inhibit its transactivity. The present study demonstrated that CCDC106 expression correlates with advanced TNM stage (P = 0.008), positive regional lymph node metastasis (P < 0.001), and poor overall survival (P < 0.001) in 183 non-small cell lung cancer cases. A549 and H1299 cells were selected as representative of CCDC106-low and CCDC106-high expressing cell lines, respectively. CCDC106 overexpression promoted A549 cell proliferation and xenograft tumor growth in nude mice, while siRNA-mediated CCDC106 knockdown inhibited H1299 cell proliferation. CCDC106 promoted AKT phosphorylation and upregulated the cell cycle-regulating proteins Cyclin A2 and Cyclin B1. Cell proliferation promoted by CCDC106 via Cyclin A2 and Cyclin B1 was rescued by treatment with the AKT inhibitor, LY294002. Our studies revealed that CCDC106 is associated with non-small cell lung cancer progression and unfavorable prognosis. CCDC106 enhanced Cyclin A2 and Cyclin B1 expression and promoted A549 and H1299 cell proliferation, which depended on AKT signaling. These results suggest that CCDC106 may be a novel target for lung cancer treatment.

CCDC85B promotes non‐small cell lung cancer cell proliferation and invasion

“…Accumulating experimental evidence has indicated that CCDC family members have a pivotal role in several processes associated with tumorigenesis and tumor progression . Nonetheless, the expression patterns and functions of CCDC85B in malignancies remain unknown.…”

“…Accumulating experimental evidence has indicated that CCDC family members have a pivotal role in several processes associated with tumorigenesis and tumor progression. [3][4][5][6] Nonetheless, the expression patterns and functions of CCDC85B in malignancies remain unknown. To the best of our knowledge, this is the first study that investigated the protein expression of CCDC85B and its correlation with the clinicopathological profiles in NSCLC.…”

“…Coiled‐coil domain containing (CCDC) family members contain coiled‐coil domains, and the proteins containing this domain function in several physiological processes, such as the regulation of signal transduction, gene expression, cell division, and cell motility . Accumulating evidence indicates that CCDC family members have diverse roles in tumorigenesis and tumor progression …”

“…1,2 Accumulating evidence indicates that CCDC family members have diverse roles in tumorigenesis and tumor progression. [3][4][5][6] CCDC85B is a 202-amino acid protein with two internal coiledcoil domains and no other recognizable domains. This protein is associated with the hepatitis delta antigen, and this interaction affects viral genomic replication; thus, it is also known as delta-interacting protein A (DIPA).…”

CCDC12 Promotes Tumor Development and Invasion Through the Snail Pathway in Colon Adenocarcinoma

“…In addition, coiled-coil domain containing family members have been associated with tumor cell proliferation. CCDC106 is associated with the progression and poor prognosis of non-small cell lung cancer (10), while CCDC67 has been demonstrated to inhibit the proliferation of papillary thyroid carcinomas (11). A genome-wide association study (GWAS) in China identi ed colorectal cancer risk single nucleotide polymorphism (SNP) rs1076394 as an expression Quantitative Trait Loci (eQTL) for CCDC12 (12).…”