CCL2 single nucleotide polymorphism of rs1024611 implicates prominence of inflammatory cascade by univariate modeling in Indian AMD

Abstract

BackgroundThe role of chemotactic protein CCL2/MCP-1 has been widely explored in age related macular degeneration (AMD) patients as well as animal models through our previous studies.AimAim of the study was to examine the association of another variance of CCL2, rs1024611 in pathophysiology of AMD.MethodsThis particular SNP has been found to be involved in inflammatory processes in various diseases. Total 171 subjects were recruited in the study with all demographic details by administering a standard questionnaire. SNP analysis was performed with TaqMan assay. Linear univariate and ANCOVA modeling was performed to show the interaction of rs1024611 with another SNP variant of CCL-2/CCR-2 (rs4586 and rs1799865) and impact of individual genotypes on CCL-2 expression in the context of AMD pathology.ResultsResults showed that both heterozygous (AG, p = 0.01) and homozygous (GG, p = 0.0001) genotypes are associated with AMD pathology. Allele frequency analysis showed that ‘G’ allele is frequent in AMD patients as compared to controls (p = 0.0001). Moreover, AMD patients who smoke were found to be associated with ‘AG’ genotype (p = 0.0145). Although, we did not find any significant interaction between the SNP variants by linear univariate analysis but results show the effect of ‘CT’ genotype on ‘TT’ genotype in rs4586 by considering rs1024611 as covariate.ConclusionBased on these results it is imperative that CCL2 mediated pathology may be associated with AMD.

Sleeping pattern and activities of daily living modulate protein expression in AMD

“…Studies have also shown that the combined effect of both alcohol consumption and smoking might further exacerbate the AMD pathology by influencing the activity of SOD (superoxide dismutase) and glutathione peroxidase activity [8]. Our previous reports have also defined the pathological role of oxidative stress [9], impaired angiogenesis [10,11] and inflammatory cascade (mediated through CCL2 and CCR3) [12][13][14]. Similar pathological hallmarks have also been exhibited by other degenerative diseases including AD, ALS etc.…”

Development and implementation of ZED-YOG quality module: Niyantrita Madhumeha Bharata skill development initiatives

“…The implementation of this module has led to enhanced visibility in the research. Research papers written from NRL provide sufficient data which shows that this quality module has improved the quality of data generated [4][5][6].…”

The impact of ICAM-1, CCL2 and TGM2 gene polymorphisms on differentiation syndrome in acute promyelocytic leukemia

“…MCP-1 regulates the migration of monocytes/macrophages to tissues and is required for response to inflammation and routine immunological surveillance of tissues [26]. An A to G single nucleotide polymorphism (SNP) in the CCL2 enhancer region (rs1024611, originally designated as -2518G or -2578G) has been associated with several chronic inflammatory conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis [26][27][28]. In the present study, no association was found between this polymorphism and the development of differentiation syndrome.…”