CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation

Piryani, Sadhna O.; Kam, Angel Y.F.; Vu, Uyen T.; Chao, Nelson J.; Doan, Phuong L.

doi:10.1016/j.stemcr.2019.04.023

Cited by 18 publications

(12 citation statements)

References 41 publications

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“…The results indicated that the concentration of IFN-γ were significantly elevated in the infertile CCL5 was an important chemokine expressed by hematopoietic and non-hematopoietic cell. [35][36][37] CCL5 expression was associated with recruitment of CD8 + T lymphocytes in tumor and acute infection. [38][39][40] In addition, CCL5 can promote the cytotoxic ability of CD8 + T lymphocytes via cytokine production during systemic chronic lymphocytic choriomeningitis virus (LCMV) infection.…”

Section: Discussionmentioning

confidence: 99%

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Altered T lymphocyte subtypes and cytokine profiles in follicular fluid associated with diminished ovary reserve

Zhao

Zhang

Ding

et al. 2022

American J Rep Immunol

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Problem: Diminished ovarian reserve (DOR) is a daunting obstacle in in vitro fertilization (IVF) or intra cytoplasmic sperm injection (ICSI), leading to poor reproductive outcomes. We aim to characterize the T cell and cytokine profiles in follicular fluid (FF) and elucidate its contribution to the development of DOR. Method of study:A total of 92 infertile women were enrolled in the study. We assessed the ultrastructure, proliferation, and apoptosis of granulosa cells (GCs). The levels of CCL5 and cytokines in FF was measured. Additionally, we classified the T cells and analyzed cytokines production in T cell. We further verified whether CCL5 can recruit specific T cell subcytes to the follicles.

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Section: Discussionmentioning

confidence: 99%

“…CCL5 was an important chemokine expressed by hematopoietic and non‐hematopoietic cell 35–37 . CCL5 expression was associated with recruitment of CD8 + T lymphocytes in tumor and acute infection 38–40 .…”

Section: Discussionmentioning

confidence: 99%

Altered T lymphocyte subtypes and cytokine profiles in follicular fluid associated with diminished ovary reserve

Zhao

Zhang

Ding

et al. 2022

American J Rep Immunol

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“…RANTES is a pluripotent cytokine and plays important roles in inflammation; it is produced by a variety of cells, such as platelets, macrophages, eosinophils, fibroblasts, endothelium, epithelia and endometrial cells [ 33 ]. A recent study reported that injection of RANTES (as an endothelial cell-secreted hematopoietic growth factor) after 5 Gy TBI promoted hematopoietic cells and increased survival of C57BL/6 mice [ 34 ]. It is currently unclear why the RANTES levels went down in the intestine after radiation exposure.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic Study of rhIL-18BP and Its Effect on Radiation-Induced Cytokine Changes in Mouse Serum and Intestine

Cui

Hull

Zizzo

et al. 2022

Toxics

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Administration of recombinant human IL-18 binding protein (rhIL-18BP), a natural antagonist of IL-18, significantly increased mouse survival after lethal doses of irradiation. To further understand the roles of IL-18BP in radiation mitigation, we studied the pharmacokinetic (PK) parameters of rhIL-18BP, and the serum and intestinal cytokine changes in CD2F1 mice treated with vehicle or rhIL-18BP after 9.0 Gy total body irradiation (TBI). For the PK study, non-compartmental pharmacokinetic analysis was performed using PKsolver. Serum and intestine specimens were collected to measure 44-cytokine levels. Principal component analysis showed a clear separation of the non-irradiated samples from the irradiated samples; and partial separation with or without rhIL-18BP treatment. Cytokine clusters that were significantly correlated in the serum or intestine, respectively were identified. On the individual cytokine levels, serum and intestinal cytokines that were significantly changed by irradiation and rhIL-18BP treatment were identified. Finally, cytokines that were significantly correlated between their serum and intestinal levels were identified. The current study established the PK parameters of rhIL-18BP in mice, identified significantly changed cytokines in mouse serum and intestine after radiation exposure and rhIL-18BP treatment. Current data provide critical insights into IL-18BP’s mechanism of action as a radiation mitigator.

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“…61 Interleukin 17 is a proinflammatory cytokine and supports the regeneration of hematopoietic progenitor cells and improves the survival of radiation damage. 62,63 Also, it promotes angiogenesis for neovascularization for injured tissue. 64 Interleukin 1α regulates the proliferation of hematopoietic stem cells and stimulates the growth factors required for hematopoiesis.…”

Section: Discussionmentioning

confidence: 99%

Allogeneic Adipose-Derived Stem Cells Mitigate Acute Radiation Syndrome by the Rescue of Damaged Bone Marrow Cells from Apoptosis

Chinnapaka

Yang

Samadi

et al. 2021

Stem Cells Translational Medicine

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Acute radiation syndrome (ARS) is the radiation toxicity that can affect the hematopoietic, gastrointestinal, and nervous systems upon accidental radiation exposure within a short time. Currently, there are no effective and safe approaches to treat mass population exposure to ARS. Our study aimed to evaluate the therapeutic potential of allogeneic adipose-derived stem cells (ASCs) for total body irradiation (TBI)-induced ARS and understand the underlying mitigation mechanism. We employed 9.25 Gy TBI dose to C57BL/6 mice and studied the effect of allogeneic ASCs on mice survival and regeneration of the hematopoietic system. Our results indicate that intraperitoneal-injected ASCs migrated to the bone marrow, rescued hematopoiesis, and improved the survival of irradiated mice.Our transwell coculture results confirmed the migration of ASCs to irradiated bone marrow and rescue hematopoietic activity. Furthermore, contact coculture of ASCs improved the survival and hematopoiesis of irradiated bone marrow in vitro. Irradiation results in DNA damage, upregulation of inflammatory signals, and apoptosis in bone marrow cells, while coculture with ASCs reduces apoptosis via activation of DNA repair and the antioxidation system. Upon exposure to irradiated bone marrow cells, ASCs secrete prosurvival and hematopoietic factors, such as GM-CSF, MIP1α, MIP1β, LIX, KC, 1P-10, Rantes, IL-17, MCSF, TNFα, Eotaxin, and IP-10, which reduces oxidative stress and rescues damaged bone marrow cells from apoptosis. Our findings suggest that allogeneic ASCs therapy is effective in mitigating TBI-induced ARS in mice and may be beneficial for clinical adaptation to treat TBI-induced toxicities. Further studies will help to advocate the scale-up and adaptation of allogeneic ASCs as the radiation countermeasure.

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CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation

Cited by 18 publications

References 41 publications

Altered T lymphocyte subtypes and cytokine profiles in follicular fluid associated with diminished ovary reserve

Altered T lymphocyte subtypes and cytokine profiles in follicular fluid associated with diminished ovary reserve

Pharmacokinetic Study of rhIL-18BP and Its Effect on Radiation-Induced Cytokine Changes in Mouse Serum and Intestine

Allogeneic Adipose-Derived Stem Cells Mitigate Acute Radiation Syndrome by the Rescue of Damaged Bone Marrow Cells from Apoptosis

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